Phylogeny of the genus Pinnixa White, 1846 (Crustacea, Brachyura, Pinnotheridae) and allies inferred from mitochondrial and nuclear molecular markers, with generic reassignment of twenty-one species Author Theil, Emma Palacios Author Felder, Darryl L. text Zoosystema 2020 2020-03-03 42 6 85 103 journal article 23751 10.5252/zoosystema2020v42a6 69e88778-6ab9-44ad-bce0-bb84eb35391b 1638-9387 3695831 urn:lsid:zoobank.org:pub:C87A10FB-E817-4293-96FD-00C2EF82D371

TABLE 2. — Primers used in this study. The length of the fragments obtained is approximate for pinnotherids, without trimming. References: 1, Crandall & Fitzpatrick Jr. 1996; 2, Schubart et al. 2001; 3, Palacios Theil et al. 2009; 4, Schubart 2009; 5, Buhay et al. 2007; 6, Svenson & Whiting 2004.

gene	primer	sequence in 5’ to 3’ direction	references	primer pair	bp obtained
16S rDNA	1472	AGA TAG AAA CCA ACC TGG	1	16S-L2	580
	16S-L2	TGC CTG TTT ATC AAA AAC AT	2	1472	580
	16S-pH1	CGC TGT TAT CCC TAA AGT AAC	3	16S-L2	415
	16S-pH2	CCT GGC TCA CGC CGG TCT GAA	3	16S-L2	570
				16S-pL1	380
	16S-pH3	AAT CCT TTC GTA CTA AAA	3	16S-pL1	430
	16S-pL1	AAC TTT TAA GTG AAA AGG CTT	3	16S-pH2	380
				16S-pH3	430
	16S-pL2	TTA CTT TAG GGA TAA CAG CG	3	NADH1	465
ND1 + tRNA-Leu	16S-L6	TTG CGA CCT CGA TGT TGA AT	3, 4	NADH1	410
	NADH1	TCC CTT ACG AAT TTG AAT ATA TCC	3, 4	16S-L6	410
				16S-pL2	465
12S rDNA	12Sf 12S1R	GAA ACC AGG ATT AGA TAC CC AGC GAC GGG CGA TAT GTA C	5 5	12S1R 12Sf	395 395
Histone 3	HexAF	ATG GCT CGT ACC AAG CAG ACG GC	6	HexAR	375
	HexAR	ATA TCC TTG GGC ATG ATG GTG AC	6	HexAF	375