First report of molecular taxonomic analyses of European beaver metazoan parasites from Hungary Author Szekeres, Sándor Department of Parasitology and Zoology, University Author Czabán, Dávid Department of Zoology, Hungarian National History Author Takács, Nóra Department of Parasitology and Zoology, University Author Széll, Zoltán National Reference Laboratory for Parasitology, Fish and Bee Diseases, National Food Chain Safety Office, Budapest, Author Gubányi, András Department of Zoology, Hungarian National History & Hungarian Natural History Museum, Main Office, Budapest, Hungary Author Kontschán, Jenő Plant Protection Institute, Centre for Agricultural Research, ELKH, Budapest, Hungary Author Hornok, Sándor Department of Parasitology and Zoology, University Author Sréter, Tamás National Reference Laboratory for Parasitology, Fish and Bee Diseases, National Food Chain Safety Office, Budapest, text Parasitology Research 2022 1895 2022-05-24 121 7 1895 1902 http://dx.doi.org/10.1007/s00436-022-07547-y journal article 10.1007/s00436-022-07547-y 1432-1955 PMC9192500 35606605 12549572

Table 1 Primers and cycle conditions of conventional PCRs used in this study

Species	Primer name	Primer sequence	Thermal profile	Reference
Stichorchis subtriquetrus	STH18F	5′-CTA AGT ACA TAC CTT TAA	95 °C for 5 min; 40x (94 °C for	Campbell-Palmer et al. 2009
	STH18R	ACG G-3′	30 s, 55 °C for 30 s, 72 °C for
		5′-CTC TAA ATG ATC AAG TTT	1 min); 72 °C for 7 min
		GG-3′
Platypsyllus castoris	LCO1490	5′-GGT CAA CAA ATC ATA	95 °C for 5 min; 40x (94 °C for	Folmer et al. 1994
	HCO2198	AAG ATA TTG G-3′	40 s, 48 °C for 1 min, 72 °C for
		5′-TAA ACT TCA GGG TGA	1 min); 72 °C for 10 min
		CCA AAA AAT CA-3′
Schizocarpus mites	bcdf05	5′-TTT TCT ACH AAY CAT	95 °C for 5 min; 40x (94 °C for	Dabert et al. 2010
	bcdR04	AAA GAT ATT GC-3′	45 s, 50 °C for 1 min, 72 °C for
		5′-TAT AAA CYT CDG GAT	1 min); 72 °C for 10 min
		GNC CAA AAA A-3′