Hypoxis limicola and H. uniflorata (Hypoxidaceae) deserve species rank: multiple new lines of evidence Author Niemann, Hendrik J. 0000-0001-9940-415X School of Animal, Plant and Environmental Sciences, University of the Witwatersrand, Johannesburg, South Africa & hennieniemann 92 @ gmail. com; https: // orcid. org / 0000 - 0001 - 9940 - 415 X hennieniemann92@gmail.com Author Coetzer, Dewald J. 0000-0001-6443-1254 School of Animal, Plant and Environmental Sciences, University of the Witwatersrand, Johannesburg, South Africa & 2650996 @ students. wits. ac. za; https: // orcid. org / 0000 - 0001 - 6443 - 1254 2650996@students.wits.ac.za Author Glennon, Kelsey L. 0000-0002-0538-7916 School of Animal, Plant and Environmental Sciences, University of the Witwatersrand, Johannesburg, South Africa & kelsey. glennon @ wits. ac. za; https: // orcid. org / 0000 - 0002 - 0538 - 7916 kelsey.glennon@wits.ac.za text Phytotaxa 2023 2023-10-05 619 1 63 85 http://dx.doi.org/10.11646/phytotaxa.619.1.3 journal article 272024 10.11646/phytotaxa.619.1.3 0b2ec9e2-7130-4b58-8f3c-bdbba00b3538 1179-3163 8425746

TABLE 4. Differences among H. membranacea, H. parvula var. parvula, H. parvula var. albiflora, H. limicola, and H. uniflorata.

	Character	H. membranacea	H. parvula var. parvula	H. parvula var. albiflora	H. limicola	H. uniflorata
	Flowers per scape	(1–)2 or 3	1(–2)	1(–2)	1(–2)	1(–2)
	Tepal colour	white	yellow	white	yellow	yellow
	Tepal length (mm)	4.5–8.0	(7–)8–10	(7–)8–10	2–6	5–8
	Filament type	filiform	filiform	filiform	filiform	subulate
Flowers	Filament colour Stigma shape	white spherical	yellow spherical	yellow spherical	yellow spherical	yellow pyramidal
	Bracts per stalk	2	0(–1)	0(–1)	(0–)1–2	1(–2)
	Bract length (mm)	3–6	1–4	1–4	5–7	ca. 2
	Articulation between scape and pedicel	inconspicuous	inconspicuous	inconspicuous	distinctly swollen	inconspicuous
	Shape	lanceolate	lanceolate	lanceolate	lanceolate	linear
Leaves	Texture Trichome distribution	membranous, adaxial surface with translucent pustules both surfaces, arises from pustules	soft to membranous, smooth or adaxial surface with translucent pustules both surfaces, arises from pustules when present	soft to membranous, smooth or adaxial surface with translucent pustules both surfaces, arises from pustules when present	soft to membranous, smooth or adaxial surface with translucent pustules both surfaces, arises from pustules when present	soft to membranous, smooth both surfaces, midrib and veins
	Trichome type	stellate interspersed with simple	bifurcate or stellate interspersed with simple	bifurcate or stellate interspersed with simple	bifurcate or stellate interspersed with simple	bifurcate or stellate
	Size (mm)	1.10–1.25 × 0.95–1.00	0.9–1.0 × 0.7–0.8	0.9–1.0 × 0.7–0.8	0.8–1.0 × 0.75–0.85	1.05–1.10 × 0.95–1.00
	Convex outer periclinal cell wall shape	columellate and/or capitate/pilate	colliculate and/or columellate	colliculate and/or columellate	colliculate and/or columellate	columellate and/or capitate/pilate
Seeds	Distribution pattern of micropapillae of different lengths	no clear pattern in distribution of different lengths across periclinal wall	shorter at top of convex projection, getting longer across periclinal wall and again getting shorter/absent towards anticlinal boundaries	shorter at top of convex projection, getting longer across periclinal wall and again getting shorter/absent towards anticlinal boundaries	gets shorter towards anticlinal boundaries	shorter at top of convex projection, getting longer across periclinal wall and again getting shorter towards anticlinal boundaries
	Micropapillae overlap	common	rare	rare	common	rare
Distribution	Range Biome preference Altitude (m.a.s.l.)	EC, KZN, MP forest/forest margin 20–900	EC, FS, KZN, LS, MP grassland 1900–2200	KZN (EC) grassland 1500–1850	LP, MP, SZ grassland 1260–1980	FS (KZN) grassland 1820–1900