| Outgroup Mercuria emiliana | Spain, Mallorca, La Puebla | AF213346 | Wilke et al. (2000) |
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| (39.79111, 3.10472) | AF212913 | |
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| Ingroup Agrafia wiktori | Greece, Evrytania, Agrafa mountains, well at Sikiá (39.36861, 21.63139) | JF906762 JF906758 | Szarowska & Falniowski (2011) |
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| Alzoniella finalina | Italy, Liguria, Savona, Molino, spring at the Porra River (44.219, 8.255) | AF367650 AF367686 | Wilke et al. (2001) |
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| Avenionia brevis | France, Gard, spring of the | AF367638 | Wilke et al. (2001) |
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| fountain of St.Victor-La Coste (44.057, 4.636) | AF367670 | |
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| Balkanica yankovi | Bulgaria, Sulari and Yantra | MF179879 | Osikowski et al. (2017) |
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| villages, Izvora Cave (42.95638, 25.31444) | MF179924 | |
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| Belgrandia thermalis | Italy, Tuscany, Pisa, | AF367648 | Wilke et al. (2001) |
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| S. Giuliano Terme, thermal channel (43.751, 10.440) | AF367684 | |
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| Belgrandiella kusceri | Slovenia, spring of Rakek (N.D.) | JX970610 JX970574 | Wilke et al. (2013) |
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| Belgrandiellopsis chorfensis gen. et sp. nov. | Tunisia, Béja Province, Chorfa Spring (36.86592, 9.36331) | MN580416 MN575710 | Present study |
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| Belgrandiellopsis chorfensis gen. et sp. nov. | Tunisia, Béja Province, Chorfa Spring (36.86592, 9.36331) | MN580417 – | Present study |
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| Belgrandiellopsis secunda gen. et sp. nov. | Tunisia, Bizerte Province, Ettoute Spring (36.883900, 9.50589) | MN580418 MN575711 | Present study |
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| Belgrandiellopsis secunda gen. et sp. nov. | Tunisia, Bizerte Province, Ettoute Spring (36.883900, 9.50589) | MN580419 MN575712 | Present study |
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| Biserta putealis gen. et sp. nov. | Tunisia, Bizerte Province, Soudene Well (37.09046, 9.7780) | MN580420 MN575713 | Present study |
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| Boetersiella sturmi | Spain, Jaén, Mata Bejid, | MH350199 | Delicado et al. (2019) |
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| La Mata Spring (37.69503, -2.49297) | MH348097 | |
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| Boleana umbilicata | Slovenia, Močilnik, the spring of the Ljubljanica River (45.95416, 14.2925) | JX982795 JX982797 | Falniowski & Szarowska (2012) |
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| Bullaregia tunisiensis | Tunisia, Province Béja, a spring in Djebba (36.47125, 9.09972) | KX821683 MN575709 | Khalloufi et al. (2017) Present study |
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| Bullaregia tunisiensis | Tunisia, Province Béja, a | MN580415 | Present study |
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| spring in Djebba (36.47125, 9.09972) | – | |
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| Cavernisa zaschevi | Bulgaria, Tserovo, Vodnata | MF179895 | Osikowski et al. (2017) |
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| Cave (45.95417, 14.2925) | MF179931 | |
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| Corbellaria celtiberica | Spain, Soria, Manubles River | MH350207 | Delicado et al. (2019) |
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| (41.60990, -1.95580) | MH348099 | |
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| Dalmatinella fluviatilis | Croatia, Jankovica Buk waterfalls, Zrmanja River (44.20272, 15.72136) | KC344541 KC344539 | Falniowski & Szarowska (2013) |
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| Daphniola graeca | Greece, Dafne Spring | EF070618 | Szarowska (2006) |
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| (39.89108, 22.60722) | EF070624 | |
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| Deganta azarum | Spain, Asturias, Borondes, | MH350208 | Delicado et al. (2019) |
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| La Fontona Spring (43.33189, -5.98506) | MH348100 | |
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| Devetakia mandrica | Bulgaria, Chavdartsi, Mandrata | MF179891 | Osikowski et al. (2017) |
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| Cave (43.24222, 24.96917) | MF179927 | |
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| Devetakiola devetakium | Bulgaria, Prevala, spring near Vreloto Cave (43.46944, 22.86111) | MF179901 MF179935 | Osikowski et al. (2017) |
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| Fissuria boui | France, Alpes Maritimes, | AF367654 | Wilke et al. (2001) |
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| Peymeinade, spring near La Prouveresse (43.64279, 6.88735) | AF367690 | |
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| Graecoarganiella parnassiana | Greece, Parnassus mountains, south of Eptalofos, small spring (38.59278, 22.5039) | JN202348 JN202341 | Falniowski & Szarowska (2011) |
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| Graziana alpestris | Italy, Liguria, Savona, Molino, spring at the Porra River (44.219, 8.255) | AF367641 AF367673 | Wilke et al. (2001) |
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| Grossuana codreanui | Romania, a spring close to the lake Techirghiol (43.99361, 28.54611) | EF061919 EF061916 | Szarowska et al. (2007) |
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| Hauffenia tellinii | Italy, Friuli-Venetia Julia, | AF367640 | Wilke et al. (2001) |
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| Gorizia, spring of the Isonzo River near Sagrado (45.8743, 13.4856) | AF367672 | |
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| Horatia klecakiana | Croatia, N of Kučiće, Studenci spring, in the valley of the Cetina River (43.44481, 16.80708) | KJ159128 KJ159127 | Szarowska & Falniowski (2014) |
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| Islamia globulus | Spain, Huesca, Gistain, Grima | MH350222 | Delicado et al. (2019) |
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| Spring (42.58660, -0.31766) | MH348103 | |
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| Kerkia kusceri | Slovenia, Ivancna Gorica, Cave Krška jama (45.89, 14.7711) | KY087867 KY087833 | Rysiewska et al. (2017) |
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| Pauluccinella minima | Italy, S. Egidio, Lago di Piediluco (42.52925, 12.7474) | JX970612 JX970578 | Wilke et al. (2013) |
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| Pontobelgrandiella angelovi | Bulgaria, a spring in Tvarditsa (42.70556, 25.89778) | KU496965 MF179938 | Rysiewska et al. (2016) Osikowski et al. (2017) |
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| Pseudamnicola lucensis | Italy, Tuscany, Bagni di Lucca, Bagni Caldi, thermal spring (44.007, 10.585) | AF367651 AF367687 | Wilke et al. (2001) |
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| Radomaniola callosa | Italy, Abruzzo, Caramanico Terme, stream on the left bank of Orfento River (42.1571, 14.0167) | AF367649 AF367685 | Wilke et al. (2001) |
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| Sadleriana fluminensis | Croatia, Jadro River at Solin near Split (43.5453, 16.48780) | AF367647 AF367683 | Wilke et al. (2001) |
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| Stoyanovia stoyanovi | Bulgaria, Bezhanovo village, Parnitsite Cave (43.22889, 24.38861) | MF179902 MF179936 | Osikowski et al. (2017) |
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| Trichonia kephalovrissonia | Greece, spring in Thérmon (38.57294, 21.66622) | EF070619 EF070630 | Szarowska (2006) |
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