Morphological and phylogenetic analyses reveal two new species and a new record of Phyllosticta (Botryosphaeriales, Phyllostictaceae) from Hainan, China Author Zhang, Zhaoxue https://orcid.org/0000-0002-4824-9716 College of Life Sciences, Shandong Normal University, Jinan, 250358, China & Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of Plant Protection, Shandong Agricultural University, Taian, 271018, China Author Liu, Xiaoyong College of Life Sciences, Shandong Normal University, Jinan, 250358, China Author Zhang, Xiuguo College of Life Sciences, Shandong Normal University, Jinan, 250358, China Author Meng, Zhe College of Life Sciences, Shandong Normal University, Jinan, 250358, China zmeng@sdnu.edu.cn text MycoKeys 2022 2022-07-04 91 1 23 http://dx.doi.org/10.3897/mycokeys.91.84803 journal article http://dx.doi.org/10.3897/mycokeys.91.84803 1314-4049-91-1 641F2C504C6A560DA710838E84A32F16

Table 1. Species and GenBank accession numbers of DNA sequences used in this study.

Species1	Voucher2	Host/Substrate	Country	GenBank accession number
				ITS	LSU	tef1	ACT	GPDH
1Newly generated sequences in this study are in bold. 2Isolates marked with "*" are ex-type or ex-epitype strains.
Phyllosticta acaciigena	CPC 28295 *	Acacia suaveolens	Australia	KY173433	KY173523	-	KY173570	-
P. aloeicola	CPC 21020 *	Aloe ferox	South Africa	KF154280	KF206214	KF289193	KF289311	KF289124
	CPC 21021	Aloe ferox	South Africa	KF154281	KF206213	KF289194	KF289312	KF289125
P. ardisiicola	NBRC 102261 *	Ardisia crenata	Japan	AB454274	AB454274	-	AB704216	-
P. aristolochiicola	BRIP 53316 *	Aristolochia acuminata	Australia	JX486129	-	-	-	-
P. azevinhi	MUCC0088	Ilex pedunculosa	Japan	AB454302	AB454302	-	AB704226	-
P. beaumarisii	CBS 535.87	Muehlenbekia adpressa	Australia	AY042927	KF306229	KF289170	KF306232	KF289074
P. brazillianiae	LGMF 330 *	Mangifera indica	Brazil	JF343572	KF206217	JF343593	JF343656	JF343758
	LGMF 333	Mangifera indica	Brazil	JF343574	KF206216	JF343595	JF343658	JF343760
P. camelliae	MUCC0059	Camellia japonica	Japan	AB454290	AB454290		AB704223
P. capitalensis	CBS 128856 *	Stanhopea graveolens	Brazil	JF261465	KF206255	JF261507	KF289289	JF343776
	CBS 226.77	Baccaurea ramiflora	Brazil	FJ538336	KF206289	FJ538394	FJ538452	JF343718
	CBS 356.52	Paphiopedilum callosum	Germany	FJ538342	KF206300	FJ538400	FJ538458	KF289087
	CBS 100175	Ilex sp.	Not given	FJ538320	KF206327	FJ538378	FJ538436	JF343699
	CBS 101228	Citrus sp.	Brazil	FJ538319	KF206325	FJ538377	FJ538435	KF289086
	CBS 114751	Nephelium lappaceum	Hawaii	EU167584	EU167584	FJ538407	FJ538465	KF289088
	CBS 115047	Vaccinium sp.	New Zealand	FJ538323	KF206318	FJ538381	FJ538439	KF289077
	CBS 115049	Aspidosperma polyneuron	Brazil	FJ538324	KF206317	FJ538382	FJ538440	KF289084
	CBS 117118	Bowdichia nitida	Brazil	FJ538339	JQ743603	FJ538397	FJ538455	KF289090
	CBS 120428	Musa acuminata	Indonesia	JN692544	KF206315	JN692532	JN692520	JN692509
	CBS 123373	Sansevieria sp.	Netherlands	FJ538341	JQ743604	FJ538399	FJ538457	JF343703
	CPC 13987	Protea repens	Portugal	KF206183	KF206281	KF289176	KF289263	KF289083
	CPC 16592	Citrus limon	Argentina	KF206187	KF206270	KF289273	KF289178	KF289092
	CPC 17468	Cymbidium sp.	Brazil	KF206188	KF206259	KF289189	KF289284	KF289120
	CPC 20256	Ophiopogon japonicus	Thailand	KC291337	KF206247	KC342557	KC342534	KF289089
	CPC 20257	Ficus benjamina	Thailand	KC291338	KF206246	KC342558	KC342535	KF289099
	LGMF219	Citrus sinensis	Brazil	KF206202	KF206220	JF261490	KF289306	JF343737
	LGMF220	Citrus sinensis	Brazil	KF206203	KF206219	JF261488	KF289307	JF343735
	LGMF222	Citrus sinensis	Brazil	KF206204	KF206218	JF261492	KF289308	JF343739
	SAUCC210144	Rhapis excelsa	China	OM571175	OM571179	OM640045	OM640047	OM640049
	SAUCC210148	Rhapis excelsa	China	OM571176	OM571180	OM640046	OM640048	OM640050
P. carochlae	CGMCC 3.17317 *	Caryota ochlandra	China	KJ847422	-	KJ847444	KJ847430	KJ847438
	CGMCC 3.17318	Caryota ochlandra	China	KJ847423	-	KJ847445	KJ847431	KJ847439
P. cavendishii	BRIP 554196 *	Musa cv. Formosana	Taiwan	JQ743562	-	KF009743	KF014080	-
	BRIP 58008	Banana	Australia	KC988365	-	KF009742	KF014071	-
P. cordylinophila	CPC 20261 *	Cordyline fruticosa	Thailand	KF170287	KF206242	KF289172	KF289295	KF289076
	CPC 20277	Cordyline fruticosa	Thailand	KF170288	KF206228	KF289171	KF289301	KF289075
P. eugeniae	CBS 445.82	Eugenia aromatica	Indonesia	AY042926	KF206288	KF289208	KF289246	KF289139
P. fallopiae	MUCC0113 *	Fallopia japonica	Japan	AB454307	AB454307	-	-	-
P. harai	MUCC0043	Aucuba japonica	Japan	AB454281	AB454281	-	AB704219	-
P. hubeiensis	CGMCC 3.14986 *	Viburnum odoratissimim	China	JX025037	-	JX025042	JX025032	JX025027
	CGMCC 3.14987	Viburnum odoratissimim	China	JX025038	-	JX025043	JX025033	JX025028
P. ilicis-aquifolii	CGMCC 3.14358 *	Ilex aquifolium	China	JN692538	-	JN692526	JN692514	-
	CGMCC 3.14359	Ilex aquifolium	China	JN692539	-	JN692527	JN692515	-
P. maculata	CPC 18347 *	Musa cv. Goly-goly pot-pot	Australia	JQ743570	-	KF009700	KF014016	-
	BRIP 46622	Musa cv. Goly-goly pot-pot	Australia	JQ743567	-	KF009692	KF014013	-
P. mangiferae	IMI 260.576 *	Mangifera indica	India	JF261459	KF206222	JF261501	JF343641	JF343748
	CPC 20260	Arecaceae	Thailand	KF206193	KF206243	KF289187	KF289294	KF289114
P. mangifera-indica	MFLUCC 10-0029 *	Mangifera indica	Thailand	KF170305	KF206240	KF289190	KF289296	KF289121
P. miurae	MUCC0065	Lindera praecox	Japan	AB454291	AB454291	-	AB704224	-
P. musaechinensis	GZAAS6.1247	Musa. sp.	China	KF955294	-	KM816639	KM816627	KM816633
	GZAAS6.1384	Musa. sp.	China	KF955295	-	KM816640	KM816628	KM816634
P. musarum	BRIP57803	Musa. sp.	Malaysia	JX997138	-	KF009737	KF014055	-
	BRIP58028	Musa. sp.	Australia	KC988377	-	KF009738	KF014054	-
P. oblongifolae	SAUCC210055	Garcinia oblongifolia	China	OM248442	OM232085	OM273890	OM273894	OM273898
	SAUCC210054	Garcinia oblongifolia	China	OM248443	OM232086	OM273891	OM273895	OM273899
	SAUCC210053	Garcinia oblongifolia	China	OM248444	OM232087	OM273892	OM273896	OM273900
	SAUCC210052 *	Garcinia oblongifolia	China	OM248445	OM232088	OM273893	OM273897	OM273901
P. paracapitalensis	CPC 26517 *	Citrus floridana	Italy	KY855622	KY855796	KY855951	KY855677	KY855735
	CPC 26518	Citrus floridana	Italy	KY855623	KY855797	KY855952	KY855678	KY855736
	CPC 26700	Citrus floridana	Italy	KY855624	KY855798	KY855953	KY855679	KY855737
	CPC 26701	Citrus floridana	Italy	KY855625	KY855799	KY855954	KY855680	KY855738
	CPC 26805	Citrus floridana	Italy	KY855626	KY855800	KY855955	KY855681	KY855739
	CPC 26806	Citrus floridana	Italy	KY855627	KY855801	KY855956	KY855682	KY855740
	CPC 28120	Citrus limon	Spain	KY855628	KY855802	KY855957	KY855683	KY855741
P. paracapitalensis	CPC 28121	Citrus limon	Spain	KY855629	KY855803	KY855958	KY855684	KY855742
	CPC 28122	Citrus limon	Spain	KY855630	KY855804	KY855959	KY855685	KY855743
	CPC 28123	Citrus limon	Spain	KY855631	KY855805	KY855960	KY855686	KY855744
	CPC 28127	Citrus limon	Spain	KY855632	KY855806	KY855961	KY855687	KY855745
	CPC 28128	Citrus limon	Spain	KY855633	KY855807	KY855962	KY855688	KY855746
	CPC 28129	Citrus limon	Spain	KY855634	KY855808	KY855963	KY855689	KY855747
P. parthenocissi	CBS 111645 *	Parthenocissus quinquefolia	USA	EU683672	-	JN692530	JN692518	-
P. partricuspidatae	NBRC 9466 *	Parthenocissus tricuspidata	Japan	KJ847424	-	KJ847446	KJ847432	KJ847440
	NBRC 9757	Parthenocissus tricuspidata	Japan	KJ847425	-	KJ847447	KJ847433	KJ847441
P. philoprina	CBS 587.69	Ilex aquifolium	Spain	KF154278	KF206297	KF289206	KF289250	KF289137
	CBS 616.72	Ilex aquifolium	Germany	KF154279	KF206296	KF289205	KF289251	KF289136
P. pterospermi	SAUCC210104 *	Pterospermum heterophyllum	China	OM249954	OM249956	OM273902	OM273904	OM273906
	SAUCC210406	Pterospermum heterophyllum	China	OM249955	OM249957	OM273903	OM273905	OM273907
P. rhizophorae	NCYUCC 19-0352 *	Rhizophora stylosa	Taiwan	MT360030	MT360039	-	MT363248	MT363250
	NCYUCC 19-0358	Rhizophora stylosa	Taiwan	MT360031	MT360040	-	MT363249	MT363251
P. schimae	CGMCC 3.14354 *	Schima superba	China	JN692534	-	JN692522	JN692510	JN692506
P. schimicola	CGMCC 3.17319 *	Schima superba	China	KJ847426	-	KJ847448	KJ847434	KJ854895
	CGMCC 3.17320	Schima superba	China	KJ847427	-	KJ847449	KJ847435	KJ854896
P. styracicola	LC1642 *	Styrax gradiflorus	China	JX025040	-	JX025045	JX025035	JX025030
P. vitis-rotundifoliae	CGMCC 3.17321	Vitis rotundifolia	USA	KJ847429	-	KJ847451	KJ847437	KJ847443
	CGMCC 3.17322 *	Vitis rotundifolia	USA	KJ847428	-	KJ847450	KJ847436	KJ847442