Cryptic diversity found in Didymellaceae from Australian native legumes Author Keirnan, Elizabeth C. School of Agriculture, Food and Wine, Waite Research Institute, The University of Adelaide, SA 5005, Australia elizabeth.keirnan@adelaide.edu.au Author Tan, Yu Pei https://orcid.org/0000-0002-4565-6148 School of Agriculture, Food and Wine, Waite Research Institute, The University of Adelaide, SA 5005, Australia Author Laurence, Matthew H. Australian Institute of Botanical Science, Royal Botanic Gardens and Domain Trust, Mrs Macquaries Rd, Sydney, NSW 2000, Australia Author Mertin, Allison A. Australian Institute of Botanical Science, Royal Botanic Gardens and Domain Trust, Mrs Macquaries Rd, Sydney, NSW 2000, Australia Author Liew, Edward C. Y. Australian Institute of Botanical Science, Royal Botanic Gardens and Domain Trust, Mrs Macquaries Rd, Sydney, NSW 2000, Australia Author Summerell, Brett A. Australian Institute of Botanical Science, Royal Botanic Gardens and Domain Trust, Mrs Macquaries Rd, Sydney, NSW 2000, Australia Author Shivas, Roger G. Department of Agriculture and Fisheries, Ecosciences Precinct, Dutton Park, QLD 4102, Australia & Centre for Crop Health, University of Southern Queensland, Toowoomba, QLD 4350, Australia text MycoKeys 2021 2021-02-08 78 1 20 http://dx.doi.org/10.3897/mycokeys.78.60063 journal article http://dx.doi.org/10.3897/mycokeys.78.60063 1314-4049-78-1 F83FEF511ABC587BBDC4A784A3A099D2
Table 1. Didymellaceae isolates examined in this study. Novel taxa and newly generated sequences are indicated in bold.
SpeciesStrain 1HostLocality 2GenBank accessions 3
ITSrpb 2
1 BRIP, Queensland Plant Pathology Herbarium, Brisbane, QLD, Australia; CBS, Westerdijk Fungal Biodiversity Institute, Utrecht, the Netherlands; CGMCC, China General Microbiological Culture Collection, Beijing, China; MFLUCC, Mae Fah Luang University Culture Collection, Chiang Rai, Thailand; UTHSC, Fungus Testing Laboratory at the University of Texas Health Science Center, San Antonio, Texas, USA. 2 NSW, New South Wales; SA, South Australia; VIC, Victoria; WA, Western Australia. 3ITS, internal transcribed spacer region; rpb 2, RNA polymerase II second subunit. T ex-type strain.
Ascochyta astragalinaCBS 113797Lathyrus vernusSwedenKT389482MT018257
Ascochyta benningiorumCBS 144957 TSoilThe NetherlandsMN823581MN824606
Ascochyta coronillae-emeriMFLUCC 13-0820 THippocrepis emerusItalyMH069661MH069679
Ascochyta fabaeCBS 524.77Phaseolus vulgarisBelgiumGU237880MT018241
Ascochyta herbicolaCBS 629.97WaterUSA, Montana, MissoulaGU237898KP330421
Ascochyta koolungaDAR 78535 TPisum sativumAustralia, SA, MinnipaEU338416EU874849
BRIP 70265Pisum sativumAustralia, SA, RivertonMN567671MN604922
BRIP 69590Pisum sativumAustralia, SA, MundullaMN567672MN604923
Ascochyta lentisCBS 370.84Lens culinarisUnknownKT389474MT018246
Ascochyta medicaginicolaCBS 112.53 TMedicago sativaUSAGU237749MT018251
Ascochyta nigripycnidiaCBS 116.96 TVicia craccaRussiaGU237756MT018253
Ascochyta phacaeCBS 184.55 TPhaca alpineSwitzerlandKT389475MT018255
Ascochyta pilosellaCBS 583.97 TClintonia unifloraCanadaMN973590MT018258
Ascochyta pisiCBS 122785Pisum sativumThe NetherlandsGU237763MT018244
Ascochyta rabieiCBS 237.37 TCicer arietinumBulgariaKT389479MT018256
Ascochyta rosaeMFLUCC 15-0063 TRubus ulmifoliusItalyKY496751KY514409
Ascochyta syringaeCBS 545.72 TSyringa vulgarisThe NetherlandsKT389483MT018245
Ascochyta versabilisCBS 876.97Silene sp.The Netherlands, WageningenGU237909KT389561
Ascochyta viciaeCBS 451.68Vicia sepiumThe Netherlands, Baarn, PraamgrachtKT389484KT389562
Ascochyta viciae-pannonicaeCBS 254.92Vicia pannonicaCzechoslovakiaKT389485MT018250
Ascochyta viciae-villosaeCBS 255.92Vicia villosaCzechoslovakiaMN973584MT018249
Didymella americanaCBS 185.85Zea maysUSA, GeorgiaFJ426972KT389594
Didymella anserinaCBS 253.80GermanyKT389498KT389595
Didymella arachidicolaCBS 333.75 TArachis hypogaeaSouth Africa, Cape ProvinceGU237833KT389598
Didymella aureaCBS 269.93 TMedicago polymorphaNew Zealand, AucklandGU237818KT389599
Didymella chlamydosporaYW23-14 TSoilSouth KoreaMK836111LC480708
Didymella coffeae-arabicaeCBS 123380 TCoffea ArabicaEthiopiaFJ426993KT389603
Didymella combretiCBS 137982 TCombretum mossambiciensisZambiaMN973525MT018139
Didymella curtisiiCBS 251.92Nerine sp.The NetherlandsFJ427038MT018131
Didymella degraaffiaeCBS 144956 TSoilThe NetherlandsMN823444MN824470
Didymella eucalypticaCBS 377.91Eucalyptus sp.Australia, WAGU237846KT389605
Didymella gardeniaeCBS 626.68 TGardenia jasminoidesIndiaFJ427003KT389606
Didymella glomerataCBS 528.66Chrysanthemum sp.The NetherlandsFJ427013GU371781
Didymella guttulataCBS 127976 TSoilZimbabweMN973524MT018138
Didymella heteroderaeCBS 109.92 TUndefined food materialThe NetherlandsFJ426983KT389601
Didymella keratinophilaUTHSC DI16-200 THomo sapiensUSALT592901LT593039
Didymella lethalisCBS 103.25GU237729KT389607
BRIP 69584Lathyrus tingitanusAustralia, SA, Brownhill CreekMN567674MN604925
Didymella magnoliaeMFLUCC 18-1560 TMagnolia grandifloraChinaMK347814MK434852
Didymella maydisCBS 588.96 TZea maysUSA, Wisconsin, HancockFJ427086GU371782
Didymella mitisCBS 443.72 TSoilSouth AfricaMN973523MT018137
Didymella musaeCBS 463.69Mangifera indicaIndiaFJ427026MT018148
Didymella nigricansCBS 444.81Acer palmatumJapanKY742075KY742158
Didymella pinodellaCBS 318.90Pisum sativumThe NetherlandsFJ427051MN983533
BRIP 69589Pisum sativumAustralia, VIC, RainbowMN567675MN604926
Didymella pinodesCBS 525.77 TPisum sativumBelgiumGU237883KT389614
BRIP 69581Senna artemisioidesAustralia, SA, BlanchetownMN567676MN604927
BRIP 69593Senna artemisioidesAustralia, SA, BlythMN567677MN604928
BRIP 69596Senna artemisioidesAustralia, SA, WudinnaMN567678MN604929
BRIP 69578Vicia craccaAustralia, NSW, CowraMN567679MN604930
Didymella pomorumCBS 539.66Polygonum tataricumThe NetherlandsFJ427056KT389618
Didymella prolaticollaCBS 126182 TSoilNamibiaMN973533MT018157
Didymella prosopidisCBS 136414 TProsopis sp.South AfricaKF777180MT018149
BRIP 69579Gastrolobium celsianumAustralia, SA, AdelaideMN5676780MN604931
Didymella protuberansCBS 381.96 TLycium halifoliumThe NetherlandsGU237853KT389620
Didymella sanctaCBS 281.83 TAilanthus altissimaSouth AfricaFJ427063KT389623
Didymella sinensisCGMCC 3.18348 TCerasus pseudocerasusChinaKY742085MT018127
Didymella subglobisporaCBS 364.91 TAnanas sativusMN973531MT018153
Didymella subglomerataCBS 110.92Triticum sp.USA, North DakotaFJ427080KT389626
Epicoccum brahmansenseCBS 990.95 TSoilPapua New GuineaMN973513MT018119
Epicoccum brasilienseCBS 120105 TAmaranthus sp.BrazilGU237760KT389627
Epicoccum camelliaeCGMCC 3.18343 TCamellia sinensisChinaKY742091KY742170
Epicoccum catenisporumCBS 181.80 TOryza sativaGuinea-BissauFJ427069LT623253
Epicoccum dendrobiiCGMCC 3.18359 TDendrobium fimbriatumChinaKY742093MT018084
Epicoccum dickmaniiCBS 124671 TAcropora FormosaAustraliaMN973509MT018113
Epicoccum djirangnandiri sp. nov.BRIP 69585 TSwainsona galegifoliaAustralia, NSW, Mount AnnanMN567673MN604924
Epicoccum draconisCBS 186.83Dracaena sp.RwandaGU237795KT389628
Epicoccum duchesneaeCGMCC 3.18345 TDuchesnea indicaChinaKY742095MT018115
Epicoccum henningsiiCBS 104.80Acacia mearnsiiKenyaGU237731KT389629
Epicoccum hordeiCGMCC 3.18360 THordeum vulgareAustraliaKY742097MT018102
Epicoccum huancayenseCBS 105.80 TSolanum sp.PeruGU237732KT389630
Epicoccum italicumCGMCC 3.18361 TAcca sellowianaItalyKY742099KY742172
Epicoccum keratinophilumUTHSC DI16-271 THomo sapiensUSALT592930LT593068
Epicoccum latusicollumCGMCC 3.18346 TSorghum bicolorChinaKY742101KY742174
Epicoccum longiostiolatumCBS 886.95 TStellaria sp.Papua New GuineaFJ427074MT018108
Epicoccum mackenzieiMFLUCC 16-0335 TOnonis spinoseItalyKX698039KX698035
Epicoccum mezzettiiCBS 173.38 TPopulus pulpItalyMN973496MT018095
Epicoccum nigrumCBS 173.73 TDactylis glomerataUSAFJ426996KT389632
Epicoccum ovisporumCBS 180.80 TZea maysSouth AfricaFJ427068LT623252
Epicoccum phragmosporaCGMCC 3.19339 TSaccharum officinarumChinaMN215619MN255460
Epicoccum pimprinumCBS 246.60 TSoilIndiaFJ427049MT018100
Epicoccum plurivorumCBS 558.81 TSetaria sp.New ZealandGU237888KT389634
Epicoccum pneumoniaeUTHSC DI16-257 THomo sapiensUSALT592927LT593065
Epicoccum poaceicolaMFLUCC 15-0448 TPoaceaeThailandKX965727KX898365
Epicoccum poaeCGMCC 3.18363 TPoa annuaUSAKY742113KY742182
Epicoccum polychromumCBS 141502 TPaspalum dilinateumFranceMN973506MT018109
Epicoccum proteaeCBS 114179 TProtea compacta x Protea neriifoliaSouth Africa, Somerset WestJQ044433LT623251
Epicoccum pseudokeratinophilumMFLUCC 18-1593 TPrunus aviumChinaMH827002MH853659
Epicoccum purpurascensCBS 128906SoilUSAMN973488MT018083
Epicoccum sorghinumCBS 179.80Sorghum bicolorPuerto RicoFJ427067KT389635
Epicoccum tobaicumCBS 384.36 TSoilIndonesiaMN973493MT018092
Epicoccum variabileCBS 119733 TCoffea ArabicaBrazilMN973501MT018103
Epicoccum viticisCGMCC 3.18344 TVitex negundoChinaKY742118KY742186
Neoascochyta desmazieri (outgroup)CBS 297.69 TLolium perenneGermany, HohenliethKT389508KT389644
Neodidymelliopsis achlydisCBS 256.77 TAchlys triphyllaCanada, British Columbia, Vancouver IslandKT389531MT018293
Neodidymelliopsis cannabisCBS 234.37Cannabis sativaUnknownGU237804KP330403
Neodidymelliopsis farokhinejadiiCBS 142853Conocarpus erectusIranKY449009KY464922
Neodidymelliopsis longicollaCBS 382.96 TSoilIsrael, En Avdat, Negev desertKT389532MT018298
Neodidymelliopsis moricolaMFLUCC 17-1063Morus albaRussiaKY684939KY684943
Neodidymelliopsis negundinisJZB380011Acer negundoRussiaMG564165MG564166
Neodidymelliopsis polemoniiCBS 109181 TPolemonium caeruleumThe NetherlandsGU237746KP330427
Neodidymelliopsis ranunculiCBS 286.72Citrus limoniumItalyMN973612MT018294
Neodidymelliopsis tillaeCBS 519.95 TTilia sp.ItalyMN973610MT018287
Neodidymelliopsis tinkyukuku sp. nov.BRIP 69592 THardenbergia violaceaAustralia, SA, ClareMN5676781MN604932
Neodidymelliopsis xanthinaCBS 383.68 TDelphinium sp.The Netherlands, BaarnGU237855KP330431
Nothophoma acaciaeCBS 143404 TAcacia melanoxylonAustraliaMG386056MG386144
Nothophoma anigozanthiCBS 381.91 TAnigozanthus maugleisiiThe NetherlandsGU237852KT389655
Nothophoma arachidis-hypogaeaeCBS 125.93Arachis hypogaeaIndia, MadrasGU237771KT389656
Nothophoma brennandiaeCBS 145912 TSoilThe NetherlandsMN823579MN824604
Nothophoma garlbiwalawarda sp. nov.BRIP 69580Senna artemisioidesAustralia, SA, AdelaideMN5676782MN604933
BRIP 69586Senna artemisioidesAustralia, SA, BerriMN5676783MN604934
Nothophoma garlbiwalawarda sp. nov.BRIP 69587Senna artemisioidesAustralia, SA, BerriMN5676784MN604935
BRIP 69594Senna artemisioidesAustralia, SA, KimbaMN5676785MN604936
BRIP 69595 TSenna artemisioidesAustralia, SA, WudinnaMN5676786MN604937
Nothophoma eucalyptigenaCBS 142535 TEucalyptus sp.AustraliaKY979771KY979852
Nothophoma gossypiicolaCBS 377.67Gossypium sp.USA, TexasGU237845KT389658
Nothophoma infossaCBS 123395 TFraxinus pennsylvanicaArgentina, Buenos Aires Province, La PlataFJ427025KT389659
Nothophoma infuscataCBS 121931 TAcacia longifoliaNew ZealandMN973559MN973559
Nothophoma macrosporaUTHSC DI16-199 THomo sapiensUSA, ArizonaLN880536LT593073
Nothophoma naiawu sp. nov.BRIP 69583 TSenna artemisioidesAustralia, SA, BlanchetownMN5676787MN604938
BRIP 69582 TSenna artemisioidesAustralia, SA, BlanchetownMN5676788MN604939
Nothophoma nullicanaCPC 32330 TAcacia falciformisAustraliaNR_156665MG386143
Nothophoma pruniMFLUCC 18-1600Prunus aviumChinaMH827005MH853662
Nothophoma quercinaCBS 633.92Microsphaera alphitoides from Quercus sp.UkraineGU237900KT389657
Nothophoma variabilisUTHSC DI16-285 THomo sapiensUSALT592939LT593078