Calicophoron daubneyi (Dinnik, 1962) (Digenea) in beef and dairy cattle in the Czech Republic: prevalence and drug efficacy Author Brutovská, Anna Baštýřová Faculty of Agriculture and Technology, University of South Bohemia in České Budějovice, Czech Republic; Author Vogalová, Pavlína Faculty of Agriculture and Technology, University of South Bohemia in České Budějovice, Czech Republic; Author Rost, Michael Faculty of Agriculture and Technology, University of South Bohemia in České Budějovice, Czech Republic; Author Sak, Bohumil Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, České Budějovice, Czech Republic; Author Kváč, Martin Faculty of Agriculture and Technology, University of South Bohemia in České Budějovice, Czech Republic; & Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, České Budějovice, Czech Republic; text Folia Parasitologica 2023 001 2023-01-02 70 1 7 http://dx.doi.org/10.14411/fp.2023.001 journal article 10.14411/fp.2023.001 1803-6465 8143705

Table 2. Faecal egg counts (FEC) of Calicophoron daubneyi (CD) and Fasciola hepatica (FH) on beef farms on days 0 and 21 after treatment by Aldifal 100 (ALD, in dose of 10 mg of albendazol per kg of body weight), Ivomec (IVC, in dose of 0.2 mg Ivermectin per kg of body weight), Closamectin (CLO, in dose of 0.2 mg of ivermectin and 5 mg closantel per kg of body weight), and Distocur (DIS, in dose 10 mg of oxyclozanide per kg of body weight) and efficiency of treatment eXpressed as the percentage decrease in eXcreted eggs after treatment. Statistically significant differences between pre-treatment and post-treatment values were assessed by ManneWhitney test (*P <0.05). Min - the smallest amount of eggs shed per gram of faeces; MaX - the greatest amount of eggs shed per gram of faeces; CI – confidence interval; ND – not defined

	Day 0 Day 21
Screening Used Fluke FEC FEC Farm ID anthel- Number of Number of term species Efficiency (%) mintics treated / treated / (CI 95%) positive animals positive animals Mean ± SD Min MaX Mean ± SD Min MaX
B7	Fall 2020 IVC CD 35/33 92 ± 89 5 405 35/33 91 ± 85 12 357 0.9 (0–37.7) Spring 2021 IVC CD 35/34 101± 92 12 409 35/34 100 ± 98 14 400 0.4 (0–37.3)
B11 B15 B20 B31 B33 B36 B42 B49 B56 B59	Spring 2021 – CD 9/5 10 ± 12 9 34 9/5 9 ± 10 12 25 6.7 (0–68.2) Spring 2021 IVC CD 25/23 26 ± 16 9 70 25/23 27 ± 15 11 56 0 (ND) Fall 2021 IVC CD 25/23 27 ± 16 11 67 25/23 26 ± 17 12 76 3.8 (0–32.4) Spring 2021 CLO CD 22/22 52 ± 29 9 109 22/22 54 ± 24 23 100 0 (ND) Fall 2021 CLO CD 22/22 51 ± 21 21 99 22/22 48 ± 21 23 111 4.1 (0–25.8) Fall 2020 IVC CD 30/30 18 ± 10 4 45 30/30 21 ± 8 6 41 0 (ND) Spring 2021 IVC CD 30/30 23 ± 10 7 48 30/30 23 ± 9 7 43 1.7 (0–21.3) Spring 2021 – CD 12/3 3 ± 5 7 14 12/3 3 ± 7 8 23 – Fall 2021 – CD 12/3 5 ± 11 3 34 12/3 4 ± 7 12 20 – Spring 2021 – CD 12/8 13 ± 14 2 43 12/8 13 ± 13 4 37 – Fall 2021 – CD 12/10 15 ± 14 3 45 12/10 14 ± 11 5 36 – Fall 2020 ALD CD 27/27 144 ± 126 23 589 27/27 130 ± 100 27 478 9.9 (0–43.1) Spring 2021 ALD CD 27/27 135 ± 98 28 400 27/27 136 ±102 24 467 0 (ND) Spring 2021 IVC CD 13/12 25 ± 16 9 51 13/12 24 ± 16 11 54 1.3 (0–41.5) Fall 2021 IVC CD 13/12 28 ± 14 17 48 13/12 27 ± 13 13 45 2.7 (0–33.9) Fall 2020 IVC CD 17/17 116 ± 84 23 265 17/17 111 ± 70 37 245 5.0 (0–40.8) Spring 2021 DIS CD 17/17 113 ± 75 34 356 17/0 0 ± 0 0 0 100 * (ND) Fall 2021 DIS CD 17/12 29 ± 25 12 89 17/0 0 ± 0 0 0 100 * (ND) Fall 2020 CLO CD 40/40 76 ± 39 13 150 40/40 76 ± 48 17 149 0 (ND) CD 40/40 78 ± 48 23 124 40/0 0 ± 0 0 0 100 * (ND) Spring 2021 DIS FH 40/5 6 ± 4 2 12 40/0 0 ± 0 0 0 100 * (ND) CD 40/38 59 ± 28 21 109 40/0 0 ± 0 0 0 100 * (ND) Fall 2021 DIS FH 40/0 0 0 0 40/0 0 ± 0 0 0 ND