Genome-level analyses resolve an ancient lineage of symbiotic ascomycetes Author Díaz-Escandón, David Department of Biological Sciences CW 405, University of Alberta, Edmonton, AB T 6 G 2 R 3, Canada Author Tagirdzhanova, Gulnara Department of Biological Sciences CW 405, University of Alberta, Edmonton, AB T 6 G 2 R 3, Canada Author Vanderpool, Dan National Genomics Center for Wildlife and Fish Conservation, Rocky Mountain Research Station, 800 E Beckwith, Missoula, MT 59812, USA Author Allen, Carmen C. G. Department of Biological Sciences CW 405, University of Alberta, Edmonton, AB T 6 G 2 R 3, Canada Author Aptroot, André Laboratório de Botânica / Liquenologia, Instituto de Biociências Universidade Federal de Mato Grosso do Sul, Avenida Costa e Silva s / n Bairro Universitário, Campo Grande, Mato Grosso do Sul CEP 79070 - 900, Brazil Author Češka, Oluna Victoria, BC, Canada Author Hawksworth, David L. Comparative Fungal Biology, Royal Botanic Gardens, Kew, Surrey TW 9 3 DS, UK & Department of Life Sciences, The Natural History Museum, Cromwell Road, London SW 7 5 BD, UK & Jilin Agricultural University, Changchun, Jilin Province 130118, China Author Huereca, Alejandro Department of Biological Sciences CW 405, University of Alberta, Edmonton, AB T 6 G 2 R 3, Canada Author Knudsen, Kerry Czech University of Life Sciences, Faculty of Environmental Sciences, Department of Ecology, Kamýcká 129, Praha-Suchdol 165 00, Czech Republic Author Kocourková, Jana Czech University of Life Sciences, Faculty of Environmental Sciences, Department of Ecology, Kamýcká 129, Praha-Suchdol 165 00, Czech Republic Author Lücking, Robert Botanischer Garten, Freie Universität Berlin, Königin-Luise-Straße 6 - 8, 14195 Berlin, Germany Author Resl, Philipp Institute of Biology, University of Graz, Universit € atsplatz 2, 8010 Graz, Austria Author Spribille, Toby Department of Biological Sciences CW 405, University of Alberta, Edmonton, AB T 6 G 2 R 3, Canada & text Current Biology 2022 2022-11-30 30 6 1 10 http://dx.doi.org/10.1016/j.cub.2022.11.014 journal article 10.1016/j.cub.2022.11.014 7358475

Table S 2. Summary statistics and comparisons of genome annotations within Pezizomycotina. Related to Figure 1 and Data S1N.

Class	Sample Size (n)	Genome Size1 (Mbp) Predicted Genes1		GC Content 2 (%)	Num. tRNA1	Unique Proteins1	Pfams2	CAZymes1	BGCs1
Arthoniomycetes	3	27.136±5.68	8479±1452	49.67±1.11	32±7	1799±904	9063±871	233±68	27±6
Dothideomycetes	24	***34.352±7.53	****10537±1769	49.48±4.40	93±61	1508±665	****11505±1484	****412±103	*31±17
Eurotiomycetes	13	32.031±8.15	*10103±1569	48.21±2.67	96±45	1608±1266	***11421±1514	***313±102	*32±15
Laboulbeniomycetes	1	14.997	4415	40.22	48	1015	5240	87	5
Lecanoromycetes	13	*35.569±8.27	*9927±1659	*46.20±3.94	64±27	1554±425	10143±1650	265±82 ****47±20
Leotiomycetes	7	***48.824±21.21	11125±3343	***42.85±5.50	157±137	2356±1294	*11189±3059	*350±169	35±20
Lichinomycetes	24	25.091±4.7	7749±1035	51.17±4.27	79±104	996±600	8644±1089	162±26	14±6
Orbiliomycetes	4	38.166±2.08	10441±1254	46.26±2.22	90±40	1553±304	10180±1125	355±60	13±5
Pezizomycetes	7 ****81.491±57.71		****14865±4707	46.52±4.81 ****820±1197		****6998±3799	10791±2594	234±65	11±4
Saccharomycetes	1	20.551	6895	48.99	524	2179	8105	127	2
Sordariomycetes	18 ****40.132±12.09		***10186±2125	51.34±3.23	****187±100	1326±578	***11309±2183	****436±161 ****40±20