Taxonomic reassessment of salamanders (genus Hynobius) from Tsushima Islands Japan, with a resurrection of Hynobius tagoi Dunn, 1923 (Amphibia: Caudata) Author Niwa, Keita 0009-0009-6442-9555 Graduate School of Human and Environmental Studies, Kyoto University, Yoshida Nihonmatsu, Sakyo-ku, Kyoto 606 - 8501, JAPAN. Present affiliation: Akita Prefectural Office, Sanno 4 - 1 - 1, Akita 010 - 8570, JAPAN. & tsushimanda 48 @ gmail. com; https: // orcid. org / 0009 - 0009 - 6442 - 9555 tsushimanda48@gmail.com Author Nishikawa, Kanto 0000-0002-6274-4959 Graduate School of Human and Environmental Studies, Kyoto University, Yoshida Nihonmatsu, Sakyo-ku, Kyoto 606 - 8501, JAPAN. Present affiliation: Akita Prefectural Office, Sanno 4 - 1 - 1, Akita 010 - 8570, JAPAN. & Graduate School of Global Environmental Studies, Kyoto University, Yoshida Honmachi, Sakyo-ku, Kyoto 606 - 8501, JAPAN. Faculty of Agriculture and Life Science, Hirosaki University, Bunkyo-cho 3, Hirosaki, Aomori 036 - 8561, JAPAN. kuroo @ hirosaki-u. ac. jp; https: // orcid. org / 0000 - 0003 - 0278 - 2758 & nishikawa. kanto. 8 v @ kyoto-u. ac. jp; https: // orcid. org / 0000 - 0002 - 6274 - 4959 nishikawa.kanto.8v@kyoto-u.ac.jp Author Matsui, Masafumi 0000-0003-2032-2528 Graduate School of Human and Environmental Studies, Kyoto University, Yoshida Nihonmatsu, Sakyo-ku, Kyoto 606 - 8501, JAPAN. Present affiliation: Akita Prefectural Office, Sanno 4 - 1 - 1, Akita 010 - 8570, JAPAN. & fumi @ zoo. zool. kyoto-u. ac. jp; https: // orcid. org / 0000 - 0003 - 2032 - 2528 fumi@zoo.zool.kyoto-u.ac.jp Author Kanamori, Sally 0000-0003-3798-2653 Graduate School of Global Environmental Studies, Kyoto University, Yoshida Honmachi, Sakyo-ku, Kyoto 606 - 8501, JAPAN. Faculty of Agriculture and Life Science, Hirosaki University, Bunkyo-cho 3, Hirosaki, Aomori 036 - 8561, JAPAN. kuroo @ hirosaki-u. ac. jp; https: // orcid. org / 0000 - 0003 - 0278 - 2758 & s. kanamori. 5328 @ gmail. com; https: // orcid. org / 0000 - 0003 - 3798 - 2653 s.kanamori.5328@gmail.com Author Kuro-O, Masaki 0000-0003-0278-2758 kuroo@hirosaki-u.ac.jp text Zootaxa 2023 2023-08-30 5339 3 201 236 http://dx.doi.org/10.11646/zootaxa.5339.3.1 journal article 265688 10.11646/zootaxa.5339.3.1 ff895335-e448-4de5-a14d-44be257c2306 1175-5326 8308966 6882297C-7C5B-428C-AABF-F671E0440674

TABLE 4. Measurements of egg sacs, clutch size, and unfertilized egg rate of each H. tsuensis, Hynobius sp., and the two populations of H. nebulosus. Mean±SD of MAESL, MIESL, ESW, ESH, and clutch size, medians of WTL and four ratio values. Ranges are shown in parentheses.

	H. tsuensis	Hynobius sp. (H. tagoi)	H. nebulosus
	(n=13)	(n=19)	Fukue Isl. (n=11)	Nagasaki (n=12)
MAESL	110.6±10.4	139.2±16.4	277.3±32.8	278.1±30.8
	(98–136.5)	(113–176.5)	(208–326)	(233.5–327)
MIESL	43.0±6.9	64.4±8.5	148.9±18.7	140.1±20.6
	(36–60)	(47–79)	(108.5–178.5)	(104.5–174.5)
ESW	12.6±1.2	13.9±1.2	15.5±0.9	17.0±1.9
	(11–15)	(11–16.5)	(14–17)	(14–21)
ESH	12.2±1.0	12.5±1.2	14.9±0.9	15.6±1.8
	(10–14)	(10–15)	(13.5–16)	(13–19.5)
WTL	17	15	0	0
	(9.5–32)	(2.5–60)	(0–8)	(0–12.5)
MIESL/MAESL (%)	37.8	46.0	54.5	50.5
	(32.4–48.0)	(40.5–55.1)	(47.8–57.9)	(44.8–53.8)
MAESL/ESW	9.3	10.0	17.3	16.6
	(6.7–10.4)	(7.6–12.7)	(14.9–21.5)	(13.1–19.5)
ESW/ESH (%)	103.7	111.1	103.7	108.8
	(95.7–110.0)	(103.8–119.2)	(100.0–107.1)	(103.3–115.4)
Clutch size (CS)	52.8±10.8 (n=8)	45.5±11.4 (n=23)	175.9±46.9 (n=9)	111.7±23.9
	(34–74)	(21–62)	(111–266)	(77–160)
Unfertilized egg rate (%) 63.5 (n=14)		2.0 (n=24)	2.5	2.7
	(37.5–80.8)	(0.0–33.3)	(0.0–30.1)	(0.0–10.7)