Review of the genus Vespina (Lepidoptera, Incurvariidae) with two new species from China and Japan Author Hirowatari, Toshiya 0000-0002-4261-1219 yagi.sadahisa@agr.kyushu-u.ac.jp Author Yagi, Sadahisa 0000-0002-4261-1219 yagi.sadahisa@agr.kyushu-u.ac.jp Author Ohshima, Issei Department of Life and Environmental Sciences, Kyoto Prefectural University, Kyoto, 606 - 8522 Japan. Author Huang, Guo-Hua 0000-0002-6841-0095 Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Changsha 410128, Hunan, China. ghhuang @ hunau. edu. cn; https: // orcid. org / 0000 - 0002 - 6841 - 0095 ghhuang@hunau.edu.cn Author Wang, Min 0000-0002-4261-1219 Department of Entomology, South China Agricultural University, Guangzhou 510640, Guangdong, China. minwang @ scau. edu. cn; https: // orcid. org / 0000 - 0001 - 5834 - 4058 yagi.sadahisa@agr.kyushu-u.ac.jp text Zootaxa 2021 2021-02-12 4927 2 209 233 journal article 7994 10.11646/zootaxa.4927.2.3 368d2d63-f7d4-48e2-bc0d-7ebb81fb68c1 1175-5326 4537002 96B9981B-01B5-4828-A4C6-E2E4A08DB8F2
TABLE 2. Primer sets used for the amplification of degraded DNA samples.
Primer nameForward/ReversePrimer sequence (5’ to 3’)Length of amplicon
GraF1– R 6 were newly designed on the basis of internal primers used in Lees et al. (2011) with the information of several gracillariid COI sequences. VesF1– R 2 were newly designed based on COI barcoding sequences obtained from fresh Vespina materials used in the present study (amplified by LCO1490/LepF1 and HCO2198/LepR1).
LCO1490/LepF1ForwardGGTCAACAAATCATAAAGATATTGG/ ATTCAACCAATCATAAAGATATTGG
GraR2ReverseGGDAYWARTCARTTWCCRAATCC181 bp
GraF1ForwardYTAGGDAMYCCNGGDTCWYTAATYGG
GraR4ReverseGTTCADCCWGTWCCWRCYCCDKWTTC208 bp
GraF3ForwardATAAGWTTYTGAYTWYTHCCHCC
GraR5ReverseGCYCCYARRATWGADGARATWCC139 bp
GraF4ForwardGAAWMHGGRGYWGGWACWGGHTGAAC
GraR6ReverseGTRATWGCWCCHGCTAAWACWGG217 bp
GraF5ForwardGGWATYTCHTCWATYYTRGGRGC
HCO2198/LepR1ReverseTAAACTTCAGGGTGACCAAAAAATCA/ TAAACTTCTGGATGTCCAAAAAATCA223 bp
VesF1ForwardCAATTGTTACRGCWCAYGCT
VesR1ReverseGCRTGRGCAATATTDGAAGA210 bp
VesF2ForwardAATTGGTGGATTYGGAAAYTG
VesR2ReverseGCYCCATTTTCAACAAATCTT102 bp