Biosynthesis of natural and novel C-glycosylflavones utilising recombinant Oryza sativa C-glycosyltransferase (OsCGT) and Desmodium incanum root proteins Author Hao, B. College of Science, Northwest A & F University, Yangling, Shaanxi 712100, China & Department of Biological Chemistry and Crop Protection, Rothamsted Research, Harpenden, Hertfordshire AL 5 2 JQ, UK Author Caulfield, J. C. Author Hamilton, M. L. Author Pickett, J. A. Author Midega, C. A. O. Author Khan, Z. R. Author Wang, J. Author Hooper, A. M. text Phytochemistry 2016 2016-05-31 125 73 87 http://dx.doi.org/10.1016/j.phytochem.2016.02.013 journal article 285687 10.1016/j.phytochem.2016.02.013 7897511c-056c-41f9-b807-372c168e612f 1873-3700 10485001

Table 3 2-Hydroxyflavanones (3) and C -glucosylflvanones (4), generated by OsCGT, introduced to D. incanum soluble root proteins with UDP-sugar donors to generate C -glucosylflavones (5, 6) or di- C -glycosylflavones (7–11). *(Hao et al., 2015).

	R3 0	R4 0	R5 0	6- and 8- C -glucosylflavones (5, 6)		6,8-Di- C -hexosylflavones (7, 8, 9)	6,8- C -Glucosyl- C -arabinosylflavones (10, 11)
3a	H	OH	H	′ ‡	4a	′ ‡	′ ‡
3b	OH	OH	H	′	4b	′	X
3d	OMe	OH	H	′	4d	′	X
3e	OMe	OH	OMe	′	4e	X	X
3f	H	OMe	H	′	4f	′	′
3g	H	H	H	′	4g	′	Trace
3h	O-CH2–	–O	H	′	4h	′	′
3i	OH	H	OH	X	4i	X	X
3j	OH	H	H	′	4j	–	–
3k	H	OEt	H	′	4k	′	X
3l	H	O(CH2)3OH	H	′	4l	X	X
3m	H	O(CH2)6OH	H	X	4m	–	–
3n	H	OPh	H	′	4n	–	–
3o	H	Me	H	′	4o	′	X
3p	H	Et	H	′	4p	′	X
3q	H	t -Bu	H	Trace	4q	–	–
3r	F	OH	H	′ ‡	4r	–	–
3s	H	CF3	H	′	4s	′	X
3t	H	F	H	′ ‡	4t	–	–
3v	Br	OH	H	X	4v	X	X
3w	H	Cl	H	′	4w	–	–
3x	Cl	OH	H	′	4x	–	–