TABLE 2 . List of ISSR primers, their sequence motifs, annealing temperatures, number of the amplified fragments generated. R= AG.
No. Primers 5’-3’ motif Annealing temperature Number of polymorphic bands Total number of bands amplified
1 MAO (CTC)4-RC 45°C 21 25
2 HB 10 (GA)6-CC 45°C 25 28
3 HB12 (CAC)3-GC 44°C 14 18
4 HB14 (CTC)3-GC 44°C 13 15
5 17898A (CA)6-AC 45°C 10 13
6 UBC807 (AG)8-T 50°C 26 29
persistent identifier: http://table.plazi.org/id/37F0666BFFEAFFEBFF60FB1EFF2BFB5C
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Published as part of Friesen, Nikolai, Herden, Tobias, Schoenfelder, Peter (2015). Allium canariense (Amaryllidaceae), a species endemic to the Canary Islands. Phytotaxa 221 (1): 1-20. DOI: 10.11646/phytotaxa.221.1.1, URL: http://dx.doi.org/10.11646/phytotaxa.221.1.1 on page 4