Bio-genetic analysis of resistance in tomato to early blight disease, Alternaria alternata Author Alizadeh-Moghaddam, Giti ** & Department of Biology, Falavarjan Branch, Islamic Azad University, Isfahan, 84517 - 31167, Iran Author Rezayatmand, Zahra Author Esfahani, Mehdi Nasr- Author Khozaei, Mahdi text Phytochemistry 2020 112486 2020-11-30 179 1 11 http://dx.doi.org/10.1016/j.phytochem.2020.112486 journal article 10.1016/j.phytochem.2020.112486 1873-3700 8293344

Table 2 ISSR primers used for detecting polymorphism among tomato genotypes including: Primers, Sequence 5ʹ→3ʹ, Annealing Temperature, Total Band Number of Polymorphism Band, Polymorphism percent, Polymorphic Information Content, Marker Index, Effective Number of Allele, Nei’ s gene diversity and Shannon’ s Information Index.

Primer	Sequence	Annealing	Total	Number of	Polymorphism	Polymorphic	Marker	Effective	Nei’ s gene	Shannon’ s
Genomic DNA extraction from young leaf tissue.The PCR-based ISSR reaction mixes were prepared using 15 ISSR primers. The DNA amplifications were programmed in a 10-m initial denaturalization phase at 94 ◦ C followed by 45 cycles of annealing each lasting 45–60 s, at a primer-specific.The process continued with an extension phase at 72 ◦ C for 2 min and with a final extension phase at 72 ◦ C for 10 min when naturalization occurs. The PCR-amplified fragments were electrophoresed on 1.5% (w/v) agarose gels.
	5’→3′	Temperature	Band	Polymorphism	Percent	information	Index	Number of	diversity	Information
		◦ C	Number	Band		content		Allele		Index
A7	(AG)10 T	54	5	5	100	0.49	2.45	1.63	0.36	0.53
UBC811	(GA)8C	54	5	4	80	0.45	1.79	1.36	0.23	0.37
A12	(GA)6CC	53	6	6	100	0.36	2.13	1.35	0.22	0.36
UBC848	(CA)8RG	54	2	1	50	0.33	0.33	1.84	0.45	0.65
UBC855	(AC)8 YT	56	10	10	100	0.45	4.45	1.49	0.30	0.46
UBC818	(CA)8G	54	5	5	100	0.43	2.16	1.40	0.27	0.43
UBC808	(AG)8C	54	4	4	100	0.50	1.98	1.21	0.16	0.29
UBC840	(GA)8 YT	54	3	3	100	0.50	1.50	1.89	0.47	0.66
UBC880	(GGAGA)3	53	10	10	100	0.44	4.38	1.47	0.28	0.44
430	(TGG)7 A	56	4	1	25	0.37	0.37	1.06	0.06	0.13
INC3 *	(AC)8 YT	54	14	14	100	0.31	4.33	1.32	0.20	0.32
Total				68	63
Average			6.18	5.73	86.82	0.42	2.35	1.46	0.27	0.42