P 450 variations bifurcate the early terpene indole alkaloid pathway in Catharanthus roseus and Camptotheca acuminata Author Miller, Justin C. Department of Chemistry, University of Illinois at Urbana-Champaign, 1201 W. Gregory Dr., 162 Edward R. Madigan Laboratory (ERML), Urbana, IL, 61801, USA Author Hollatz, Allison J. Author Schuler, Mary A. text Phytochemistry 2021 112626 2021-03-31 183 1 13 http://dx.doi.org/10.1016/j.phytochem.2020.112626 journal article 264545 10.1016/j.phytochem.2020.112626 d3a8c867-b60b-4414-bacc-7f4dc0f256fb 1873-3700 8291734

Table 2 Kinetic parameters estimated from steady-state kinetics.

CYP	Substrate	K M	V max	k cat	k cat/ K M
		mM	μ M min 1	min 1	min 1 mM 1
72A564	loganic acid	2.67 ± 0.80	3.49 ± 0.53	13.9 ± 2.1	5.2 ± 1.7
	loganin	0.242 ± 0.023	2.997 ± 0.075	11.99 ± 0.30	49.5 ± 4.8
72A565	loganic acid	3.57 ± 0.42	4.86 ± 0.32	19.4 ± 1.3	5.45 ± 0.74
	loganin	0.722 ± 0.068	1.92 ± 0.13	15.28 ± 0.52	21.2 ± 2.1