Towards a revision of the bamboo corals (Octocorallia): Part 4, delineating the family Keratoisididae Author Watling, Les School of Life Sciences, University of Hawaii at Manoa, Honolulu, Hawaii 96822 Author Saucier, Esprit Heestand Faculty of Science, Brigham Young University - Hawaii, 55 - 220 Kulanui St, Laie, Hawaii, 96762, USA Author France, Scott C. Department of Biology, University of Louisiana at Lafayette, Lafayette, Louisiana, USA text Zootaxa 2022 2022-01-27 5093 3 337 375 journal article 2385 10.11646/zootaxa.5093.3.4 e7f4500d-24b9-4dfb-9967-46d032dad854 1175-5326 5909954 58B73298-6A33-4040-B898-ED3D8A69DA81

TABLE 1. Primers used in successful PCR and cycle sequencing. Most primer combinations were selected to cross a gene boundary, shown in the first two columns. Primers are arranged into those used to amplify mtMutS-5’, mtMutS-3’, nuclear 18S, and the other mitochondrial gene junctions. Primers that were previously published are denoted by footnotes.

			Primer 1		Primer 2
TABLE 1. Primers used in successful PCR and cycle sequencing. Most primer combinations were selected to cross a gene boundary, shown in the first two columns. Primers are arranged into those used to amplify mtMutS-5’, mtMutS-3’, nuclear 18S, and the other mitochondrial gene junctions. Primers that were previously published are denoted by footnotes.
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1 Pante et al. 2013; 2 Brugler & France, 2008; 3 Pante et al. 2012; 4 France, 2007; 5 France & Hoover, 2002; 6 McFadden et al. 2011; 7 Apakupakul et al. 1999
Gene 1	Gene 2	Name	Sequence	Name	Sequence	Annealing Temperature
mtMutS-5’
nad4L	mtMutS	nd4L2475F2	tagttttactggcctctac	mutChry3458R3	tgaagyaaaagccactcc	48-55°C
cox3	mtMutS	CO3Bam5657F2	gctgctagttggtattggcat	mutChry3458R3	tgaagyaaaagccactcc	50-55°C
mtMutS-3’
mtMutS	16S	MutS4759F4	tgtagctcatgatattag	16S5PR4	tcacgtccttaccgatag	50-55°C
Nuclear 18S
18S	18S	18S-Cf7	cggtaattccagctccaatag	18S-Yr7	cagacaaatcgctccaccaac	52-55°C
Mt genome Gene boundaries
cox1	12S	COILA9312F	gcggcttgacacccckatgttgtgggctattgg	12 S12935 R	acgacggccatgcaatacc	53°C
12S	nad1	12 S12935 F	ggtattgcatggccgcgt	nd1Bam11227R	atcagctagtacgaacctggct	52°C
12S	nad1	12S-306F	gcaaacaggattagataccc	nd1Bam11227R	atcagctagtacgaacctggct	50-51°C
nad1	nad6	nd13209F1	ggtrcgagcttctttcccyag	nd64699R1	ggattcggcgtagatataacc	60-62°C
nad1	nad4	nd13209F1	ggtrcgagcttctttcccyag	nad4gen4974F2	taggyttatttactcatacwat	49-50°C
nad1	cob	nd13209F1	ggtrcgagcttctttcccyag	cybBAM1279R	ggttcctctactggattggctcct	48-50°C
cob	nad6	cybGen402f	gattaccggaatattcttagctatgc	nd64699R1	ggattcggcgtagatataacc	61°C
nad6	nad4L	nd6BAM1878F	gctaagcagccaaatwcacct	nd4Lp6109R	ccataatggctaagccgatag	50°C
nad6	nad3	nd6BAM1878F	gctaagcagccaaatwcacct	nd3genR	aggmtcaaayccacaytcataarc	56°C
nad6	nad3	nd6BAM1878F	gctaagcagccaaatwcacct	ND32280R	ataacaacccaatatccaaa	48-50°C
nad3	nad4L	nd3genF	gyttatgartgtggrtttgakcc	nd4Lp6109R	ccataatggctaagccgatag	53-55°C
nad3	nad4L	nd32280F	tttggatattgggttgttat	nd4Lp6109R	ccataatggctaagccgatag	45-49°C
16S	nad2	16S874F	cttctggctgctgcaaag	nd2genR	gcccacatatgaaanggagc	51°C
nad2	nad5	nd2genF	gctccntttcatatgtgggc	nd5Bam2494R	gggtcwtctctctcatataacttgta	58-59°C
Gene 1	Gene 2	Name	Sequence	Name	Sequence	Annealing Temperature
nad2	nad5	nd2BAM1248F	gtgttaggggcactwtcttcgggg	nd5BAM2638R	ctgccttgttagcttgtatacgtg	50°C
nad5	nad4	nd5Bam3091F	agttattgcttattctacttgtagtca	nd4Bam4479R	cagctttatyttctctgccc	58-59°C
nad5	nad4	nd53gen13736F	agtaayttacaatctggtytagtt	nd4gen4953R	atwgtatgagtaaataarccta	47-50°C
nad5	nad4	nd5-136F	tatgttatgaaatccttattaatggg	nd4-1092R	agaaaataggggcatagtcataac	48-50°C
nad4	cox3	nd4Bam5446F	gagttarttcgtgtatgtttgcct	CO3Bam5657F2	gctgctagttggtattggcat	61°C
nad4	nad4L	nd42599F5	gccattatggttaactattac	nd4Bam5446F	gagttarttcgtgtatgtttgcct	50°C
cox3	cob	CO3Bam5657F2	gctgctagttggtattggcat	cytb3741R1	gtccgttttcatgtagcttcc	55°C
atp6	cox3	ATP66458F	cctaaccgatggcaagc	CO3Bam5876R	gcttgtagggctgtaaaytgaacc	50-53°C
atp6	cox3	ATP6-285F	gtatttaccccaactgccc	CO3Bam5876R	gcttgtagggctgtaaaytgaacc	50°C
cox2	atp6	CO2Bam7585F	atggaygctgtrcctggacgtc	atp66458R	gcttgccatcggttagg	50°C
cox2	atp6	CO2Bam7585F	atggaygctgtrcctggacgtc	atp6-392R	ccactaggcatcatcatt	49-51°C
cox2	cox3	CO2Bam7585F	atggaygctgtrcctggacgtc	CO3Bam5876R	gcttgtagggctgtaaaytgaacc	58°C
cox2	cox1	COII8068xF6	ccataacaggrctwgcagcatc	COIoctR6	atcatagcatagaccatacc	47-50°C
cox2	cox1	COII8068xF6	ccataacaggrctwgcagcatc	CO1p269R	gaaaggccatatcgggtgcac	51°C