A small slug from a tropical greenhouse reveals a new rathouisiid lineage with triaulic tritrematic genitalia (Gastropoda: Systellommatophora) Author Manganelli, Giuseppe Author Lesicki, Andrzej Author Benocci, Andrea Author Barbato, Debora Author Miserocchi, Danio Author Pieńkowska, Joanna R. Author Giusti, Folco text Zoological Journal of the Linnean Society 2023 2022-07-25 197 1 76 103 http://dx.doi.org/10.1093/zoolinnean/zlac054 journal article 54747 10.1093/zoolinnean/zlac054 c3b0da78-1f8c-4a7e-aaea-4683afb6c1a7 0024-4082 7474139 20C04023-1BB3-4AE6-787-64E36AC5C0F0

Table 2. List of gene fragments (with their acronyms) and sequences of their primers used in molecular analysis

Primer Sequence 5′–3′	References
COI – mitochondrial gene of cytochrome c oxidase subunit I, 5′-end
LCO1490 GGTCAACAAATCATAAAGATATTGG	Folmer et al. (1994)
HC02198 TAAACTTCAGGGTGACCAAAAAATCA
16S rDNA – mitochondrial gene for RNA of large ribosome subunit
16Scs1 AAACATACCTTTTGCATAATGG	Chiba (1999)
16Scs2 AGAAACTGACCTGGCTTACG
18S rDNA – nuclear gene for rRNA of small ribosome subunit
PCR amplification and sequencing
18S-A1 CTGGTTGATCCTGCCAGTCATATGC	Vonnemann et al. (2005)
18S-1800 GATCCTTCCGACGGTTCACCTACG
18S rDNA – nuclear gene for rRNA of small ribosome subunit
Additional sequencing with the use of internal primers
18S-400F ACGGGTAACGGGGAATCAGGG	Vonnemann et al. (2005)
18S-400R CCCTGATTCCCCGTTACCCGT
18S-700F GTCTGGTGCCAGCAGCCGCG
18S-700R CGCGGCTGCTGGCACCAGAC
ITS2 – internal transcribed spacer 2 in nuclear gene for rRNA of large ribosome subunit with its flanking
fragments (5.8S and 28S rDNA)
LSU1 CTAGCTGCGAGAATTAATGTGA	Wade & Mordan (2000)
LSU3 ACTTTCCCTCACGGTACTTG
H3 – nuclear gene of histone 3
H3F ATGGCTCGTACCAAGCAGAC(ACG)GC	Colgan et al. (1998)
H3R ATATCCTT(AG)GGCAT(AG)AT(AG)GTGAC