New record of Nausithoe werneri (Scyphozoa, Coronatae, Nausithoidae) from the Brazilian coast and a new synonymy for Nausithoe maculata Author Molinari, Clarissa Garbi Departamento de Zoologia, Instituto de Biociencias, Universidade de Sao Paulo, Rua do Matao, travessa 14, n. 101, Cidade Universitaria, Sao Paulo, SP, 05508 - 090, Brazil https://orcid.org/0000-0003-4086-6020 clarissamolinari@gmail.com Author Maronna, Maximiliano Manuel Departamento de Zoologia, Instituto de Biociencias, Universidade de Sao Paulo, Rua do Matao, travessa 14, n. 101, Cidade Universitaria, Sao Paulo, SP, 05508 - 090, Brazil https://orcid.org/0000-0002-2590-639X Author Morandini, Andre Carrara Departamento de Zoologia, Instituto de Biociencias, Universidade de Sao Paulo, Rua do Matao, travessa 14, n. 101, Cidade Universitaria, Sao Paulo, SP, 05508 - 090, Brazil & Centro de Biologia Marinha, Universidade de Sao Paulo, Rodovia Manuel Hypolito do Rego km 131.5, Sao Sebastiao, SP, 11600 - 000, Brazil https://orcid.org/0000-0003-3747-8748 text ZooKeys 2020 984 1 21 http://dx.doi.org/10.3897/zookeys.984.56380 journal article http://dx.doi.org/10.3897/zookeys.984.56380 1313-2970-984-1 22EB0B217A2743FBB90258061BA59B73 2BB6C926882B598F8D940855A5CEC0DE

Table 3. List of primers used to amplify each gene. *LEM = Laboratorio de Evolucao Molecular (IB-USP).

Gene	Primer	Primer sequence 5'-3'	F/R	Temp. - base pairs	Source
COI	COXl-F2	TCGACTAATCATAAAGATATCGGCAC	F	52 °C - 26 bp	Ward et al. 2005
	MEDCOXR	TGGTGNGCYCANACNATRAANCC	R	52 °C - 23 bp	Lawley et al. 2016
	LCO1490JJ4	CIACIAAYCAYAARGAYATYGG	F	55 °C+45 °C - 22 bp	Astrin et al. 2016
	HCO2198JJ4	ANACTTCNGGRTGNCCAAARAATC	R	55 °C+45 °C - 25 bp	Astrin et al. 2016
	LCO1490JJ2	CHACWAAYCAYAARGAYATYGG	F	60 °C+45 °C - 22 bp	Astrin et al. 2016
	HCO2198JJ2	ANACTTCNGGRTGNCCAAARAATCA	R	60 °C+45 °C - 25 bp	Astrin et al. 2016
28S	F15	CTAACAAGGATTCCCCTAGTAACGGCGAG	F	55.5 °C - 30 bp	LEM *
	R798	GGTCCGTGTTTCAAGACGG	R	55.5 °C - 19 bp	Medina et al. 2001
	F798	CCGTCTTGAAACACGGACC	F	55.5 °C - 19 bp	Medina et al. 2001
	R1446	GTTGTTACACACTCCTTAGCGG	R	55.5 °C - 22 bp	Medina et al. 2001
18S	18S - A	AACCTGGTTGATCCTGCCAGT	F	54 °C - 21 bp	Medlin et al. 1988
	18S - L	CCAACTACGAGCTTTTTAACTG	R	54 °C - 22 bp	Apakupakul et al. 1999
	18S - C	CGGTAATTCCAGCTCCAATAG	F	54 °C - 21 bp	Apakupakul et al. 1999
	18S - Y	CAGACAAATCGCTCCACCAAC	R	54 °C - 21 bp	Apakupakul et al. 1999
	18S - O	AAGGGCACCACCAGGAGTGGAG	F	54 °C - 22 bp	Apakupakul et al. 1999
	18S - B	TGATCCTTCCGCAGGTTCACCT	R	54 °C - 22 bp	Medlin et al. 1988