Ancient Grasshoppers: A revision of the genus Bullacris (Orthoptera: Pneumoridae) Author Gordon, Mikhaila L. Department of Biodiversity and Conservation Biology, University of the Western Cape, Bellville, 7535, South Africa. & Kirstenbosch Research Centre, South African National Biodiversity Institute, Newlands, 7725, South Africa. & Research and Exhibitions Department, South African Museum, Iziko Museums of South Africa, P. O. Box 61, Cape Town, 8000, South Africa. Author Colville, Jonathan F. Statistics in Ecology, Environment and Conservation, Department of Statistical Science, University of Cape Town, Rondebosch, 7700, South Africa. Author Engelbrecht, Adriaan 0000-0001-8846-4069 Department of Biodiversity and Conservation Biology, University of the Western Cape, Bellville, 7535, South Africa. adengelbrecht@uwc.ac.za Author Couldridge, Vanessa C. K. 0000-0003-1948-6584 Department of Biodiversity and Conservation Biology, University of the Western Cape, Bellville, 7535, South Africa. vcouldridge@uwc.ac.za text Zootaxa 2024 2024-07-01 5474 4 301 354 http://dx.doi.org/10.11646/zootaxa.5474.4.1 journal article 299654 10.11646/zootaxa.5474.4.1 88fa5369-eacb-4703-8c6a-4698220be381 1175-5326 12681191 90CD8BF7-76E7-4310-A909-F5408DA6278A

TABLE 16: A list of primers used in this study. Internal primers (e.g., COIa–F), were created using Geneious v. 7.1.3.

Primer Name	Primer Sequence
LCO1490	5’–GGTCAACAAATCATAAAGATATTGG–3’
HCO2198	5’–TAAACTTCAGGGTGAGGG TGACCAAAAAATCA–3’
ITS–F	5’–AGAGGAAGTAAAAGTCGTAACAAGG–3’
ITS–R	5’–CCTTAGTAATATGCTTAAATTCAGG–3’
18S–F	5’–TGCTTGTCTCAAAGATTAAGC–3’
18S–R	5’–GCATCACAGACCTGTTATTGC–3’
COIa–F	5’– WCCATTAATRATTGGAGCACCA3’
COIb–R	5’–RATDGGGTCACCYCCTCCTGC–3’
ITSa–F	5’–ACCGACTGCATATCCGAACG–3’
ITSb–R	5’–CTGCGTTCTTCATCGACCCA–3’
18Sa–F	5’–GATCGCACGGTCTCTGTACC–3’
18Sb–R	5’–CCTCGACACTCGGTGAAGAG–3’