Biosynthesis of cannflavins A and B from Cannabis sativa L Author Rea, Kevin A ∗ & Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, N 1 G 2 W 1, Canada Author Casaretto, José A. Author Al-Abdul-Wahid, M. Sameer Author Sukumaran, Arjun Author Geddes-McAlister, Jennifer Author Rothstein, Steven J. Author Akhtar, Tariq A. text Phytochemistry 2019 2019-05-28 164 162 171 http://dx.doi.org/10.6084/m9.figshare.24741938.v1 journal article 284782 10.1016/j.phytochem.2019.05.009 b6b9be71-bc68-4ec7-a82e-93da86a5eccd 1873-3700 10483096

Table 1 Kinetic parameters of CsPT3 with various C. sativa flavone substrates.

Substrate	DMAPP		GPP
Measurements were made at 37 ̊C in 100 mM Tris-HCl, pH 9.0 and 10 mM MgCl 2. Reactions were initiated by adding either DMAPP or GPP as the prenyl donor substrate to each reaction. Data are the means of three independent determinations ± SE. Kinetic constants were not determined (N.D.) for assays with luteolin as a flavone substrate due to low conversion rates.
	Km (μM)	V max (pmol min −1 mg −1)	Km (μM)	V max (pmol min −1 mg −1)
Apigenin	141.7 ± 15.6	1.2 ± 0.0566	49.4 ± 11.4	1.3 ± 0.0993
Chrysoeriol	37.9 ± 5.59	1.3 ± 0.0585	35.6 ± 10.7	1.3 ± 0.122
Luteolin	N.D.	N.D.	N.D.	N.D.