Use of ITS- 1 to identify Bactrocera dorsalis and Bactrocera occipitalis (Diptera: Tephritidae): a case study using flies trapped in California from 2008 to 2018 Author Barr, Norman B. USDA APHIS PPQ Mission Laboratory, 22675 North Moorefield Road, Edinburg, Texas 78541, USA; E-mail: Norman. B. Barr @ usda. gov (N. B. B.), Jennifer. Belcher @ usda. gov (J. B.) Norman.B.Barr@usda.gov Author Hauser, Martin California State Collection of Arthropods, Plant Pest Diagnostics Branch, California Department of Food and Agriculture, 3294 Meadowview Road, Sacramento, California 95832 - 1448, USA; E-mail: martin. hauser @ cdfa. ca. gov (M. H.), peter. kerr @ cdfa. ca. gov (P. K.), sgaimari @ gmail. com (S. G.) martin.hauser@cdfa.ca.gov Author Belcher, Jennifer USDA APHIS PPQ Mission Laboratory, 22675 North Moorefield Road, Edinburg, Texas 78541, USA; E-mail: Norman. B. Barr @ usda. gov (N. B. B.), Jennifer. Belcher @ usda. gov (J. B.) & Department of Biology, University of Texas Rio Grande Valley, 1201 West University Drive, Edinburg, Texas 78539, USA; E-mail: dasalinas 92 @ protonmail. com (D. S.), erin. schuenzel @ utrgv. edu (E. S.) Norman.B.Barr@usda.gov Author Salinas, David Department of Biology, University of Texas Rio Grande Valley, 1201 West University Drive, Edinburg, Texas 78539, USA; E-mail: dasalinas 92 @ protonmail. com (D. S.), erin. schuenzel @ utrgv. edu (E. S.) dasalinas92@protonmail.com Author Schuenzel, Erin Department of Biology, University of Texas Rio Grande Valley, 1201 West University Drive, Edinburg, Texas 78539, USA; E-mail: dasalinas 92 @ protonmail. com (D. S.), erin. schuenzel @ utrgv. edu (E. S.) dasalinas92@protonmail.com Author Kerr, Peter California State Collection of Arthropods, Plant Pest Diagnostics Branch, California Department of Food and Agriculture, 3294 Meadowview Road, Sacramento, California 95832 - 1448, USA; E-mail: martin. hauser @ cdfa. ca. gov (M. H.), peter. kerr @ cdfa. ca. gov (P. K.), sgaimari @ gmail. com (S. G.) martin.hauser@cdfa.ca.gov Author Gaimari, Stephen California State Collection of Arthropods, Plant Pest Diagnostics Branch, California Department of Food and Agriculture, 3294 Meadowview Road, Sacramento, California 95832 - 1448, USA; E-mail: martin. hauser @ cdfa. ca. gov (M. H.), peter. kerr @ cdfa. ca. gov (P. K.), sgaimari @ gmail. com (S. G.) martin.hauser@cdfa.ca.gov text Florida Entomologist 2021 2021-08-16 104 2 96 106 http://dx.doi.org/10.1653/024.104.0205 journal article 10.1653/024.104.0205 1938-5102 13684728

Table 2. The frequencies of the 15 ITS‑1 types recorded for Bactrocera dorsalis according to data sets.

Type	No. CA individuals	No. NCBI GenBank individuals	Frequency of types including ambiguous data	Frequency of types excluding ambiguous data	GenBank type example
A	377	145	72.20%	76.65%	KC446776.1
B	24	33	7.88%	8.37%	KC446914.1
C	21	11	4.43%	4.70%	KC446807.1
D	15	0	2.07%	2.20%	MT602821
E	4	0	0.55%	0.59%	MT602812
F	2	0	0.28%	0.29%	MT603056
G	0	7	0.97%	1.03%	KC446794.1
H	0	2	0.28%	0.29%	KC446803.1
I	0	2	0.28%	0.29%	KC446810.1
J	0	1	0.14%	0.15%	KC446930.1
K	0	1	0.14%	0.15%	KM453350.1
L	0	1	0.14%	0.15%	KM453393.1
M	0	1	0.14%	0.15%	KM453401.1
N	0	1	0.14%	0.15%	KM453406.1
O	0	1	0.14%	0.15%	KC446820.1
Ambiguous	60	14	10.24%	NA	—
Total	503	220	723	649	—