Molecular phylogenetic data and seed coat anatomy resolve the generic position of some critical Chenopodioideae (Chenopodiaceae - Amaranthaceae) with reduced perianth segments Author Sukhorukov, Alexander P. Author Nilova, Maya V. Author Krinitsina, Anastasiya A. Author Maxim A. Zaika, Author Erst, Andrey S. Author Shepherd, Kelly A. text PhytoKeys 2018 109 103 128 http://dx.doi.org/10.3897/phytokeys.109.28956 journal article http://dx.doi.org/10.3897/phytokeys.109.28956 1314-2003-109-103 486C2F59FFDB2B0C7C29B92D6A41E05D 1476320

Table 2. Primers and cycler programmes used for the molecular analysis.

Marker	Primer sequences and combination	Reference	Cycler programmer
ITS	ITS5 5'-GGA AGT AAA AGT CGT AAC AAG G-3'	White et al. (1990)	95 °C for 5 min, 33 cycles of amplification (95 °C for 15 s, 55 °C for 30 s, 72 °C for 40 s), 72 °C for 5 min
	ITS4 5'-TCC TCC GCT TAT TGA TAT GC-3'
rbcL (partial)	rbcLaF 5'- ATG TCA CCA CAA ACA GAG ACT AAA GC-3'	Levin et al. (2003)	95 °C for 5 min, 35 cycles of amplification (95 °C for 10 s, 55 °C for 30 s, 72 °C for 40 s), 72 °C for 5 min
	rbcLaR 5'-GTA AAA TCA AGT CCA CCR CG-3'	Kress et al. (2009)
atpB-rbcL spacer	atpB-rbcL F 5'-GAA GTA GTA GGA TTG ATT CTC-3'	Golenberg et al. (1993)	95 °C for 5 min, 35 cycles of amplification (95 °C for 20 s, 56 °C for 30 s, 72 °C for 60 s), 95 °C for 20 s, 56 °C for 80 s, 72 °C for 8 min
	atpB-rbcL R 5'-CAA CAC TTG CTT TAG TCT CTG-3'
trnL-F	Tab C 5'-CGA AAT CGG TAG ACG CTA CG-3'	Taberlet et al. (1991)	95 °C for 5 min, 35 cycles of amplification (95 C for 1 min, 50 °C - 65 °C (increasing in 0.3 C per cycle) for 1 min, 72 °C for 4 min), 72 °C for 5 min
	Tab D 5'-GGG GAT AGA GGG ACT TGA AC-3'
	Tab E 5'- GGT TCA AGT CCC TCT ATC CCC-3'
	Tab F 5'ATI' TGA ACT GGT GAC ACG AG 3'