Geographic isolation and human-assisted dispersal in land snails: a Mediterranean story of Helix borealis and its relatives (Gastropoda: Stylommatophora: Helicidae) Author Korábek, Ondřej Author Kosová, Tereza Author Dolejš, Petr Author Petrusek, Adam Author Neubert, Eike Author Juřičková, Lucie text Zoological Journal of the Linnean Society 2021 2021-01-30 193 4 1310 1335 https://doi.org/10.1093/zoolinnean/zlaa186 journal article 3111 10.1093/zoolinnean/zlaa186 f55bcb9b-ff76-463c-b36f-884987a1da43 0024-4082 5761476

Table 1. The primer pairs used for amplification and sequencing

Forward name	Forward sequence	Reverse name	Reverse sequence	Reference Target
LCO1490	GGTCAACAAATCATAAAGATATTGG	HC02198	TAAACTTCAGGGTGACCAAAAAATCA	Folmer et al., 1994 COI
16Scs1	AAACATACCTTTTGCATAATGG	16Scs2	TAAGCGTAGGGCTCATAAC	Chiba, 1999 16S
16Scs1	AAACATACCTTTTGCATAATGG	16S_MN3R	GCTACCTTTGCACAGTCAGWG	Chiba, 1999; 5’ half of the 16S
				Neiber et al.,
				2017
16S-F	CGGCCGCCTGTTTATCAAAAACAT	16S-R	GGAGCTCCGGTTTGAACTCAGATC	Misof et al., 2000 3’ half of the 16S
16S-F	CGGCCGCCTGTTTATCAAAAACAT	16S-Helcentr-R	AAGYTTCTAGGGTCTTCTCGTCT	Misof et al., 2000; part of the 3’ half of the
				Korábek et al., 16S
				2015
16S-Helinter-F	GTACYYTGACTGTGCAAAGGT	16S-Helinter-R	CTAGTCCAACATCGAGGTCAC	Korábek et al., part of the 3’ half of the
				2015 16S
16S-Helcentr-F	AGACGAGAAGACCCTAGAARCTT	16S-R	GGAGCTCCGGTTTGAACTCAGATC	Korábek et al., part of the 3’ half of the
				2015; Misof et al., 16S
				2000
ceratina-f	GGCGACCCCATCCTATACCA	ceratina-r	GGAAGGCCTATCACCCCTAAAC	this study 3’ of COI – t-Val – 5’ of
				16S, forward specific to
				presumed COI
pseudo-f	TCTTTACCTGTTTTAGCAGGCG	ceratina-r	GGAAGGCCTATCACCCCTAAAC	this study 3’ of COI – t-Val – 5’ of
				16S, forward specific to
				the presumed NUMT
ceratina-f2	CGCTGGGAGTTAGGTACATCT	ceratina-r2	AACCTAGCACACCGATTGAA	this study COI region overlapping
				the position of primer
				ceratina-f
H3PulF	GGAGGCAAGGCCCCACGTAARCA	H3PulR	TTGGCGTGGATGGCGCACARG	Uit de Weerd & histone H3
				Gittenberger,
				2013