Amaryllidaceae alkaloids from Narcissus pseudonarcissus L. cv. Dutch Master as potential drugs in treatment of Alzheimer's disease Author Hulcová, Daniela ∗ & ADINACO Research Group, Department of Pharmaceutical Botany, Faculty of Pharmacy, Charles University, Heyrovského 1203, 500 05, Hradec Králové, Czech & Department of Pharmacognosy, Faculty of Pharmacy, Charles University, Heyrovského 1203, 500 05, Hradec Králové, Czech Republic Author Maříková, Jana Author Korábečný, Jan Author Hošťálková, Anna Author Jun, Daniel Author Kuneš, Jiří Author Chlebek, Jakub Author Opletal, Lubomír Author Simone, Angela De Author Nováková, Lucie Author Andrisano, Vincenza Author Růžička, Aleš Author Cahlíková, Lucie text Phytochemistry 2019 2019-09-30 165 1 9 http://dx.doi.org/10.1016/j.phytochem.2019.112055 journal article 286710 10.1016/j.phytochem.2019.112055 38410415-c902-4071-9cb0-b77f32823623 1873-3700 10483285

Table 1 1 H-NMR and 13 C-NMR data of new alkaloid 6 (diastereomeric ratio 1:1.1) isolated from Narcissus pseudonarcissus cv. Duch Master.

POSITION	NARCIMATULINE
Spectra were acquired in chloroform-d at 500 MHz (1 H) and 125.7 MHz (13 C); 1 J values are in parentheses and reported in Hz; chemical shifts are given in ppm.
	δC of	δH of isomer A	δC of	δH of isomer B
	isomer A		isomer B
1	29.7	2.39–2.26 m	29.6	2.39–2.26 m
		1.84–1.64 m		1.84–1.64 m
2	35.7	2.39–2.26 m	35.6	2.39–2.26 m
		2.13–1.98 m		2.13–1.98 m
3	209.2		209.2
4	40.0	2.60 dd, overlapped	40.0	2.56 dd, overlapped
		(18.4, 2.9)		(18.0, 2.9)
4a	88.3	4.68 t (2.9)	88.2	4.55 t (2.9)
5a	146.7		146.7
6	143.6		143.6
7	110.9	6.65 d, overlapped	111.0	6.62 d, overlapped
		(8.0)		(8.0)
8	122.23	6.44 d (8.0)	122.20	6.56 d (8.0)
8a	129.9		129.9
8b	132.0		132.0
9	57.6	3.88 d, overlapped	57.9	3.98 d (15.0)
		(15.0)		3.66 d (15.0)
		3.51 d (15.0)
11	52.3	3.05–2.88 m	51.1	3.05–2.88 m
				2.86–2.74 m
12	37.4	2.13–1.98 m	36.4	1.84–1.64 m
		1.56–1.50 m		1.38–1.31 m
13	47.5		47.4
1′	54.9	3.45 d (14.2)	53.7	3.35 bs
		3.33–3.24 m
2′	122.8		122.8
3′	108.7	7.01 s	108.4	7.07 s
3a′	146.9		146.9
5′	101.0	6.00–5.98 m	100.9	6.00–5.98 m
6a′	146.0		145.8
7ֹ’	110.5	6.74 s	110.3	6.77 s
8′	133.6		133.4
9′	131.7		131.4
10′	130.4	6.83 d (7.4)	130.2	6.71 d, overlapped
				(7.4)
11′	117.9	6.68 t, overlapped	117.9	6.62 t, overlapped
		(7.4)		(7.4)
12′	123.22	7.03 d, overlapped	123.24	7.03 d, overlapped
		(7.4)		(7.4)
12a′	131.0		131.0
13′	28.6	3.05–2.88 m	28.5	3.05–2.88 m
		2.86–2.74 m		2.86–2.74 m
14′	57.1	3.33–3.24 m	56.9	3.33–3.24 m
		3.18 dd (17.5, 8.8)		3.09 dd (18.4, 9.2)
15a′	150.5		150.4
N15′-CH3	38.8	2.25 s	38.7	2.20 s
C6-OCH3	55.96	3.86–3.83 m	55.95	3.86–3.83 m