An inordinate fondness for inconspicuous brown frogs: integration of phylogenomics, archival DNA analysis, morphology, and bioacoustics yields 24 new taxa in the subgenus Brygoomantis (genus Mantidactylus) from Madagascar Author Scherz, Mark D. Author Crottini, Angelica CIBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, InBIO Laboratório Associado, Campus de Vairão, Universidade do Porto, 4485 - 661 Vairão, Portugal Author Hutter, Carl R. 0000-0001-6381-6339 Museum of Natural Sciences and Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA Ễcarl. hutter @ gmail. com; https: // orcid. org / 0000 - 0001 - 6381 - 6339 USAỄcarl.hutter@gmail.com Author Hildenbrand, Andrea Hauptstr. 13, 82234 Wessling, Germany Ễandrea @ gutachten-hildenbrand. de GermanyỄandrea@gutachten-hildenbrand.de Author Andreone, Franco Museo Regionale di Scienze Naturali, Via G. Giolitti, 36, 10123 Torino, Italy Author Fulgence, Thio Rosin 0000-0001-7205-7282 Natural and Environmental Sciences, Regional University Centre of the SAVA Region (CURSA), Antalaha, Madagascar Ễthiorosinf @ yahoo. fr; https: // orcid. org / 0000 - 0001 - 7205 - 7282 MadagascarỄthiorosinf@yahoo.fr Author Köhler, Gunther Author Ndriantsoa, Serge Herilala Author Ohler, Annemarie Author Preick, Michaela Author Rakotoarison, Andolalao Mention Zoologie et Biodiversité Animale, Université d’Antananarivo, BP 906, Antananarivo, 101 Madagascar Author Rancilhac, Loïs Author Raselimanana, Achille P. Mention Zoologie et Biodiversité Animale, Université d’Antananarivo, BP 906, Antananarivo, 101 Madagascar Author Riemann, Jana C. Author Rödel, Mark-Oliver Author Rosa, Gonçalo M. Author Streicher, Jeffrey W. Author Vieites, David R. Author Köhler, Jörn Author Hofreiter, Michael Author Glaw, Frank Author Vences, Miguel text Megataxa 2022 2022-12-15 7 2 113 311 http://dx.doi.org/10.5281/zenodo.6687412 journal article 217510 10.11646/megataxa.7.2.1 8af27bde-abaa-4f0a-8f84-8b6cc8a97034 2703-3090 7441023 2FD8C310-6486-4592-92F6-5EB894EBD6AC

TABLE 1. Sequencing and assembly metrics of target-capture sequencing of a segment of the 16S rRNA gene from liquid-preserved type specimens of historical nomina in the Mantidactylus subgenera Brygoomantis, Ochthomantis, and Hylobatrachus. Results for the latter two subgenera are here reported as the listed types were studied to exclude that they may refer to species of Brygoomantis. Assembly length is here reported not counting missing data in discontinuous assemblies. HT, holotype; LT, lectotype; PLT, paralectotype; ST, syntype. * according to lectotype designated herein. # only two separate short fragments recovered. ## probably all consisting of contamination, including human mtDNA. † MNHN 1894.001 is probably not a paralectotype of Rana aluta; see the relevant discussion, below.

Nomen	Specimen	Status	Year of collection	Reads (raw)	Reads (filtered)	Reference for assembly	Reads (assembly)	Length assembly
Subgenus Brygoomantis
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Rana aluta Peracca, 1893	MNHN 1894.001	topotypical specimen†	≤ 1893	3001331	29993	M. alutus (M. betsileanus)	2045 (2041)	157
Mantidactylus ambohimitombi Boulenger, 1918	BMNH 1947.2.26.25	LT *	1896	3302614	475894	M. betsileanus	221328	412
Mantidactylus bellyi Mocquard, 1895	MNHN 1983.240	HT	≤ 1893	3937013	97140	M. bellyi (M. betsileanus)	31627 (30700)	415
Rana betsileana Boulenger, 1882	BMNH 1947.2.26.44	PLT *	≤ 1882	3691121	321477	M. betsileanus	155396	411
Rana betsileana Boulenger, 1882	BMNH 1947.2.26.45	LT *	≤ 1882	3578636	310519	M. betsileanus	87650	411
Rana biporus Boulenger, 1889	BMNH 1947.2.26.47	LT *	≤ 1889	2299994	7578	M. betsileanus	6443	411
Mantidactylus bourgati Guibé, 1974	MNHN 1972.437	HT	1971	4044068	947438	M. bourgati (M. betsileanus)	186096 (174994)	409
Mantidactylus brauni Ahl, 1929	ZMB 53737	LT *	≤ 1929	1509708	96634	M. betsileanus	25925	414
Mantidactylus brunneus Ahl, 1929	ZMB 30514	HT	≤ 1929	3266458	240372	M. betsileanus	42034	411
Rana curta Boulenger, 1882	BMNH 1947.2.10.30	LT *	≤ 1882	2572258	200410	M. betsileanus	39260	406
Rhacophorus fumigatus Mocquard, 1895	MNHN 1895.258	HT	≤ 1895	3783324	313634	M. betsileanus	95158	411
Rana inaudax Peracca, 1893	MZUT An727.1	LT	≤ 1893	2678644	4772	M. alutus	4354	61 + 46 #
Mantidactylus madecassus Millot and Guibé, 1950	MNHN 1953.246	LT	1949	4592034	20642	M. betsileanus	12086	390
Mantidactylus multiplicatus Boettger, 1913	SMF 6733	HT	≤ 1905	3350467	262017	M. betsileanus	127565	411
Gephyromantis tricinctus Guibé, 1947	MNHN 1931.0026	LT	1930	2675582	84	M. betsileanus	10	107 ##
Mantidactylus tripunctatus Angel, 1930	MNHN 1931.0024	LT *	1926	4238117	515476	M. betsileanus	220630	413
Limnodytes ulcerosus Boettger, 1880	SMF 6605	LT	≤ 1880	2424827	86456	M. betsileanus	14935	413
Subgenus Ochthomantis
Mantidactylus catalai Angel, 1935	MNHN 1935.153	HT	≤ 1935	1451886	13	M. femoralis	0	0
Rana femoralis Boulenger, 1882	BMNH 1947.2.22.65	LT	≤ 1882	3622032	529123	M. femoralis	218031	267
Rana flavicrus Boulenger, 1889	BMNH 1947.2.26.53	HT	≤ 1885	2738970	107749	M. femoralis	65613	267
Mantidactylus majori Boulenger, 1896	BMNH 1947.2.10.26	PLT	≤ 1896	3706029	368200	M. femoralis	124909	264
Mantidactylus mocquardi Angel, 1929	MNHN 1929.207	HT	≤ 1929	4199370	451270	M. femoralis	184481	268
Mantidactylus poissoni Angel, 1937	MNHN 1937.001	HT	≤ 1937	3207653	114	M. femoralis	8	132
Subgenus Hylobatrachus
Rana cowanii Boulenger, 1882	BMNH 1947.2.22.63	ST	≤ 1882	2936459	113638	M. femoralis	54749	267
Polypedates lugubris Duméril, 1853	MNHN 1994.1752	ST	≤ 1853	3762908	379894	M. femoralis	195992	269