Two new species of Ophiostomatales (Sordariomycetes) associated with the bark beetle Dryocoetes alni from Poland Author Strzalka, Beata Department of Forest Ecosystems Protection; University of Agriculture in Krakow, Al. 29 Listopada 46, 31 - 425, Krakow, Poland Author Jankowiak, Robert Department of Forest Ecosystems Protection; University of Agriculture in Krakow, Al. 29 Listopada 46, 31 - 425, Krakow, Poland https://orcid.org/0000-0002-2804-5396 rljankow@cyf-kr.edu.pl Author Bilanski, Piotr Department of Forest Ecosystems Protection; University of Agriculture in Krakow, Al. 29 Listopada 46, 31 - 425, Krakow, Poland https://orcid.org/0000-0002-2750-6699 Author Patel, Nikita Department of Microbiology, Buller Building 213, University of Manitoba, Winnipeg, R 3 T 2 N 2, Canada Author Hausner, Georg Department of Microbiology, Buller Building 213, University of Manitoba, Winnipeg, R 3 T 2 N 2, Canada Author Linnakoski, Riikka Natural Resources Institute Finland (Luke), Latokartanonkaari 9, 00790, Helsinki, Finland Author Solheim, Halvor Norwegian Institute of Bioeconomy Research, P. O. Box 115, 1431, As, Norway text MycoKeys 2020 68 23 48 http://dx.doi.org/10.3897/mycokeys.68.50035 journal article http://dx.doi.org/10.3897/mycokeys.68.50035 1314-4049-68-23 663957445ABA5BBDA2F314785BC9A8C9
Table 1. Fungal isolates used in the present study.
Species1Isolate no2Herbarium no3HostInsect vectorOriginGenBank accession no4
CMWCBSKFL and NRIFTURITS1-5.8S-ITS2-28SITS2-28STUB2TEF1-αACTCAL
1Boldtype = new species in the present study. 2CMW Culture Collection of the Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria, South Africa; CBS Westerdijk Fungal Biodiversity Institute, Utrecht, The Netherlands; KFL Culture collection of the Department of Forest Pathology, Mycology and Tree Physiology; University of Agriculture in Krakow, Poland; NRIF The Natural Resources Institute Finland (Luke), Helsinki, Finland; WIN the University of Manitoba (Winnipeg); Canada. H = ex-holotype 3TFU the TUR Herbarium of the University of Turku, Finland 4ITS1-5.8S-ITS2-ITS2-28S = the internal transcribed spacer 1 and 2 regions of the nuclear ribosomal DNA gene, 5.8S rRNA gene; and the 28S large subunit of the nrDNA gene; ACT= Actin; TUB2 = Beta-tubulin; CAL= Calmodulin; TEF1-α = Translation elongation factor 1-alpha; Bold type = GenBank accession numbers of sequences obtained in the present study. 5Duck Mountain Provincial Forest, Manitoba, Canada.
Taxon 1
Ceratocystiopsis synnemata sp. nov.16216DAhttp://mus.utu.fi/TFU.207991Alnus incanaDryocoetes alniReskoMN900984MN901005MN901014not obtainednot obtained
13418DAhttp://mus.utu.fi/TFU.207992Populus tremulaDryocoetes alniPaprociceMN900985MN901006MN901015not obtainednot obtained
149a18DAhttp://mus.utu.fi/TFU.207993Populus tremulaDryocoetes alniPaprociceMN900986MN901007MN901016not obtainednot obtained
149b18DAhttp://mus.utu.fi/TFU.207994Populus tremulaDryocoetes alniPaprociceMN900987MN901008MN901017not obtainednot obtained
16918DAHhttp://mus.utu.fi/TFU.207995Populus tremulaDryocoetes alniPaprociceMN900988MN901009MN901018not obtainednot obtained
17718DAhttp://mus.utu.fi/TFU.207996Populus tremulaDryocoetes alniPaprociceMN900989MN901010MN901019not obtainednot obtained
Taxon 2
Leptographium alneum sp. nov.5206714490513116RJDAhttp://mus.utu.fi/TFU.207559Alnus incanaDryocoetes alniReskoMN900990MH283185MH283218MH283406MN901029MN901041
5207214490416016bRJDAhttp://mus.utu.fi/TFU.207997Alnus incanaDryocoetes alniReskoMN900991MH283219MH283407MN901030MN901042
14490316216bRJDAhttp://mus.utu.fi/TFU.207998Alnus incanaDryocoetes alniReskoMN900992MH283186MH283220MH283408MN901031MN901043
520707617RJDAPopulus tremulaDryocoetes alniPaprociceMN900993MH283221MN901020MN901032MN901044
520751449027717RJDAhttp://mus.utu.fi/TFU.207558Populus tremulaDryocoetes alniPaprociceMN900994MH283222MN901021MN901033MN901045
520698417RJDAPopulus tremulaDryocoetes alni /PaprociceMN900995MH283223MN901022MN901034MN901046
8617RJDAPopulus tremulaDryocoetes alniPaprociceMN900996MH283224MN901023MN901035MN901047
52076H144901H8917RJDAHhttp://mus.utu.fi/TFU.207557Populus tremulaDryocoetes alniPaprociceMN900997MH283225MN901024MN901036MN901048
9117RJDAPopulus tremulaDryocoetes alniPaprociceMN900998MH283226MN901025MN901037MN901049
88616RJSMMalus sylvestris.Scolytus maliRozpucieMN900999MH283187MH283227MH283409MN901038MN901050
5207114490088716aRJSMhttp://mus.utu.fi/TFU.207556Malus sylvestrisScolytus maliRozpucieMN901000MH283188MH283228MH283410MN901039MN901051
Leptographium piriforme52066297NBRZ16AOBetula pendulaWoundZohatynMN901001MH740931MH740984MH741134not obtainednot obtained
10618DAAlnus incanaDryocoetes alniPaprociceMN901002MN901011MN901026not obtainednot obtained
Grosmannia crassivaginata134119144unknownunknownunknownMN901003MN901012MN901027MN901040not obtained
Ceratocystiopsis pallidobrunneaWIN(M) 51Populus tremuloidesunknownDuck Mountain5MN901004MN901013MN901028not obtainednot obtained