Hemiaustroboletus, a new genus in the subfamily Austroboletoideae (Boletaceae, Boletales) Author Ayala-Vasquez, Olivia https://orcid.org/0000-0002-8970-9571 Tecnologico Nacional de Mexico, Instituto Tecnologico de Ciudad Victoria, Blvd. Emilio Portes Gil # 1301 Pte., Ciudad Victoria, Tamaulipas, CP 87010, Mexico Author Garcia-Jimenez, Jesus https://orcid.org/0000-0001-9290-1460 Tecnologico Nacional de Mexico, Instituto Tecnologico de Ciudad Victoria, Blvd. Emilio Portes Gil # 1301 Pte., Ciudad Victoria, Tamaulipas, CP 87010, Mexico Author Aguirre-Acosta, Elvira https://orcid.org/0000-0003-1360-7859 Instituto de Biologia, Universidad Nacional Autonoma de Mexico, Circuito exterior s / n Ciudad Universitaria, Ciudad de Mexico, CP 04510, Mexico Author Castro-Rivera, Rigoberto https://orcid.org/0000-0002-9083-1363 CIBA, Instituto Politecnico Nacional, Tlaxcala, CP 90700, Mexico Author Angeles-Argaiz, Rodolfo Enrique https://orcid.org/0000-0002-1225-2227 Instituto de Biologia, Universidad Nacional Autonoma de Mexico, Circuito exterior s / n Ciudad Universitaria, Ciudad de Mexico, CP 04510, Mexico Author Saldivar, Angel Emmanuel https://orcid.org/0000-0002-4503-3186 Departamento de Botanica y Zoologia, Universidad de Guadalajara, Zapopan, Jalisco, CP 45101, Mexico Author Garibay-Orijel, Roberto https://orcid.org/0000-0002-6977-7550 Instituto de Biologia, Universidad Nacional Autonoma de Mexico, Circuito exterior s / n Ciudad Universitaria, Ciudad de Mexico, CP 04510, Mexico rgaribay@ib.unam.mx text MycoKeys 2022 2022-03-30 88 55 78 http://dx.doi.org/10.3897/mycokeys.88.73951 journal article http://dx.doi.org/10.3897/mycokeys.88.73951 1314-4049-88-55 05D8DD51FFB350529DC32FC1ACA6F148

Table 1. List of species, geographic origin and GenBank accession numbers of ITS, LSU and RPB2 sequences used in the phylogenetic analyses.

Taxa	Voucher	Country	ITS	LSU	RPB2	Reference
Aureoboletus betula		USA		MK601736	MK766298	Kuo and Ortiz-Santana (2020)
A. garciae	MEXU:29006	Mexico		MH337251	MT228983	Haelewaters et al. (2020)
Austroboletus amazonicus	1839_ AMV	Colombia	KF937307	KF714508		Vasco-Palacios et al. (2014)
A. amazonicus	1914_ AMV	Colombia	KF937308	KF714509		Vasco-Palacios et al. (2014)
A. austrovirens	BRI:AQ0795791	Australia	KP242211	KP242225	KP242133	Fechner et al. (2017)
A. austrovirens	BRI:AQ0794622	Australia	KP242210			Fechner et al. (2017)
A. austrovirens	MEL:2382920a	Australia		KP242284	KP242113	Fechner et al. (2017)
A. austrovirens	BRI:AQ0794609	Australia		KP242226	KP242131	Fechner et al. (2017)
A. austrovirens	BRI:AQ0794171	Australia		KP242227	KP242133	Fechner et al. (2017)
A. eburneus	REH9487	Australia		JX889668		Vasco-Palacios et al. (2014)
A. dictyotus	HKAS59804	China		JX901138		Hosen et al. (2013)
A. fusisporus	HKAS75207	China	JX889719	JX889720		Hosen et al. (2013)
A. fusisporus	JXSB0351	China		MK765810		GenBank
A. gracilis	112-96	USA		DQ534624		Binder and Hibbett (2006)
A. gracilis	TM03_434	Canada		EU522815		Porter et al. (2008)
A. gracilis var. gracilis	CFMR BOS-547	USA		MK601715	MK766277	Kuo and Ortiz-Santana (2020)
A. gracilis var. flavipes	CFMR BOS-562	USA		MK601714		Kuo and Ortiz-Santana (2020)
A. gracilis	ACAD11344F	Canada	MH465078			Young et al. (2019)
A. gracilis	SFC20140823-02	South Korea	MN794901			GenBank
A. gracilis	NAMA 2017-106	USA	MH979242			GenBank
A. gracilis	310751	Mexico	MH167935			GenBank
A. gracilis	CNV35	USA	MT345212			Victoroff (2020)
A. cf. gracilis	JLF6600	USA	MN174796			GenBank
A. lacunosus	REH9146	Australia		JX889669		Vasco-Palacios et al. (2014)
A. lacunosus	MEL2233764	Australia		KC552056		GenBank
A. mucosus	TH6300	Guyana		AY612798		Drehmel et al. (2008)
A. mutabilis	BRI:AQ0795793	Australia	KP242169	KP242263	KP242098	Fechner et al. (2017)
A. mutabilis	BRI:AQ0669270	Australia		KP242266	KP242097	Fechner et al. (2017)
A. mutabilis	BRI:AQ0796266	Australia		KP242262	KP242099	Fechner et al. (2017)
A. niveus	312	New Zealand		DQ534622		Binder and Hibbett (2006)
A. niveus	MEL2053830	Australia	KC552016	KC552058		Orihara et al. (2016)
A. novae-zelandiae	PDD:72542	New Zealand	HM060327			GenBank
A. rarus	BRI:AQ0794045	Australia	KP242197	KP242236	KP242086	Fechner et al. (2017)
A. rostrupii	TH8189	Guyana	JN168683			Smith et al. (2011)
Austroboletus sp.	BRI:AQ0794156	Australia		KP242235	KP242115	GenBank
Austroboletus sp.	BRI:AQ0794222	Australia		KP242234	KP242106	GenBank
Austroboletus sp.	BRI:AQ0794271	Australia		KP242259	KP242102	GenBank
Austroboletus sp.	HKAS 57756	China		KF112383	KF112764	Wu et al. (2014)
Austroboletus sp.	HKAS 59624	China		KF112485	KF112765	Wu et al. (2014)
Austroboletus sp.	HKAS 74743	China		KT990527	KT990367	Wu et al. (2014)
Austroboletus sp.	PERTH6658407	Australia		KP242277	KP242126	GenBank
Austroboletus sp.	BRI:AQ0794242	Australia			KP242087	GenBank
Austroboletus sp.	OR0891	Thailand			MH614753	Vadthanarat et al. (2019)
Austroboletus sp.	OTAFUNNZ2013434	New Zealand			KP191670	GenBank
A. subflavidus	JBSD130771	Dominican Republic		MT580902	MT590754	Gelardi et al. (2020)
A. subflavidus	JBSD130772	Dominican Republic		MT580903	MT590755	Gelardi et al. (2020)
A. subflavidus	CFMR BZ-3178	Belize		MK601716	MK766278	Kuo and Ortiz-Santana (2020)
A. subvirens	KPM-NC-0017836	Japan		JN378518		Orihara et al. (2012)
A. viscidoviridis	Perth 7588682	Australia		KP242282	KP242128	Fechner et al. (2017)
Boletellus indistinctus	HKAS77623	China		KT990531	KT990371	Wu et al. (2016)
Boletellus sp.	HKAS80554			KT990535	KT990374	Wu et al. (2016)
Boletus harrisonii	MICH: KUO-09071204	USA		MK601718	MK766280	Kuo and Ortiz-Santana (2020)
Boletus sp.	dd08055	China	FJ810161			GenBank
Boletus sp.	MHM165	Mexico	EU569243			Morris et al. (2008)
Boletales sp.	B0229	Canada	KY825985			GenBank
Fistulinella campinaranae var. scrobiculata	AMV1980	Colombia		KF714520		Vasco-Palacios et al. (2014)
F. gloeocarpa	JBSD130769	Dominican Republic		MT580906	MT590756	Gelardi et al. (2020)
F. gloeocarpa	CFMR:B4	Bahamas		MT580904		Gelardi et al. (2020)
F. gloeocarpa	CFMR:B10	Bahamas		MT580905		Gelardi et al. (2020)
F. prunicolor	REH9502	Australia		JX889648	MG212630	Halling et al. (2012)
F. olivaceoalba	HKAS 53432	Vietnam		MH745969		GenBank
F. olivaceoalba	LE312004	Vietnam		MH718396		GenBank
F. ruschii	CORT:TJB-8329	USA		MT580907		Gelardi et al. (2020)
F. viscida	238 25S	New Zealand		AF456826		Vasco-Palacios et al. (2014)
F. cinereoalba	TH8471	Guyana		GQ477439	KT339237	GenBank
Hemiaustroboletus vinaceobrunneus	MEXU_30051 Holotype	Mexico	MN178797	MN200222	MT887617	This study
H. vinaceobrunneus	MEXU_30052 Isotype	Mexico	MN178798	MN200223	MT887618	This study
H. vinaceobrunneus	MEXU_30053 Isotype	Mexico	MN178799		MT887619	This study
H. vinaceus	AV524 Paratype	Mexico	MN178802	MN200225	MT887622	This study
H. vinaceus	AES334 Holotype	Mexico	MN178800	MN200224	MT887620	This study
H. vinaceus	AES364 Isotype	Mexico	MN178801		MT887621	This study
Hemiaustroboletus sp.	AK_3508	Mexico	MN178803			This study
Hemileccinum subglabripes	MICH: KUO-08301402	USA		MK601739	MK766301	Kuo and Ortiz-Santana (2020)
Hortiboletus rubellus	MICH: KUO-06081002	USA		MK601741	MK766303	Kuo and Ortiz-Santana (2020)
H. amygdalinus	HKAS54166	China		KT990581	KT990416	Wu et al. (2016)
Hourangia cheoi	Tang572	China		KP136953	KP136985	Zhu et al. (2015)
Imleria badia	MICH: KUO-09110404	USA		MK601743	MK766305	Kuo and Ortiz-Santana (2020)
Mucilopilus castaneiceps	HKAS 75045	China		KF112382	KF112735	Wu et al. (2016)
M. castaneiceps	HKAS50338	China		KT990555	KT990391	Wu et al. (2016)
M. castaneiceps	HKAS71039	China		KT990547	KT990385	Wu et al. (2016)
Parvixerocomus pseudoaokii	HKAS 80480	China		KP658468	KP658470	Wu et al. (2016)
Porphyrellus castaneus	HKAS52554	China		KT990697	KT990502	Wu et al. (2016)
P. porphyrosporus	MB97-023	Germany		DQ534643	GU187800	Binder and Hibbett (2006)
P. orientifumosipes	HKAS53372	China		KT990629	KT990461	Wu et al. (2016)
Tengioboletus sp.	HKAS 77869	China		KT990658	KT990483	Wu et al. (2016)
Strobilomyces confusus	CFMR:DR-3024	Dominican Republic		MK601809	MK766365	Kuo and Ortiz-Santana (2020)
Tylopilus felleus	CFMR: BOS-780	USA		MK601814	MK766370	Kuo and Ortiz-Santana (2020)
T. sordidus	MICH: KUO-06240801			MK601815	MK766371	Kuo and Ortiz-Santana (2020)
Tylopilus sp.	HKAS 50229	China		KF112423	KF112734	Wu et al. (2014)
Uncultured mycorrhizal	BOLETE1	USA	AY656925			Walker et al. (2005)
Uncultured mycorrhizal	clon N_1	South Korea	AB571507			Obase et al. (2012)
Uncultured Boletus	isolate: YM490	Japan	LC175482			Miyamoto et al. (2018)
Uncultured Boletus	Clon ZE2	China	GU391428			Ma et al. (2010)
Veloporphyrellus alpinus	KUN:HKAS68301	China		JX984537		Li et al. (2014)
V. pseudovelatus	KUN: HKAS59444	China		JX984542		Li et al. (2014)
V. pseudovelatus	KUN:HKAS52244	China		JX984531		Li et al. (2014)
V. conicus	CFMR:BZ1670	Belize		JX984543		Li et al. (2014)
V. conicus	CFMR:BZ1705	Belize		JX984544		Li et al. (2014)
V. pantoleucus	F:Gomez21232	Costa Rica		JX984548		Li et al. (2014)
V. velatus	KUN: HKAS63668	China		JX984546		Li et al. (2014)
V. aff. velatus	HKAS 57490	China		KF112380	KF112733	Wu et al. (2014)
V. vulpinus	LE315544	Vietnam	MN511177	MN511170		GenBank
V. vulpinus	LE315549	Vietnam	MN511180			GenBank
V. vulpinus	LE315546	Vietnam	MN511179			GenBank
V. vulpinus		Vietnam	MN511178			GenBank
Xerocomellus chrysenteron	HKAS:56494	China		KF112357	KF112685	Wu et al. (2014)