Set to its place: first DNA data on freshwater tardigrades of Sakhalin (Far East Russia) shed light on the phylogenetic position of Mixibius (Eutardigrada: Hypsibioidea) Author Tumanov, Denis V. Department of Invertebrate Zoology, Faculty of Biology, Saint Petersburg State University, Universitetskaya nab., 7 - 9, Saint Petersburg, 199034, Russia. & Marine Research Laboratory, Zoological Institute of the Russian Academy of Sciences, Universitetskaya nab., 1, Saint Petersburg, 199034, Russia. text Zootaxa 2026 2026-01-19 5750 1 87 108 https://doi.org/10.11646/zootaxa.5750.1.4 journal article 315262 10.11646/zootaxa.5750.1.4 15c1449b-04b9-48e1-ab43-03a16f54d3b7 1175-5326 19170183 9788C125-E7F6-44D5-80F3-9C63A3F3A7DB

TABLE 2. Primers and PCR programs used for amplification of the four DNA fragments sequenced in the study.

DNA fragment	Primer name	Primer direction	Primer sequence (5’–3’)	Primer source	PCR programme
COI	LCO1490_JJ	forward	CHACWAAYCATAAAGATATYGG	Astrin & Stüben 2008	Michalczyk et al. 2012
	HCO2198_JJ	reverse	AWACTTCVGGRTGVCCAAARAATCA	Astrin & Stüben 2008
18S rRNA	18S_Tar_Ff1	forward	AGGCGAAACCGCGAATGGCTC	Stec et al. 2017	Zeller (2010),
	18S_Tar_Rr1	reverse	GCCGCAGGCTCCACTCCTGG	Stec et al. 2017	in Stec et al. 2015
28S rRNA	28S_Eutar_F	forward	ACCCGCTGAACTTAAGCATAT	Gąsiorek et al. 2018b	Mironov et al. 2012
	28S_R0990	reverse	CCTTGGTCCGTGTTTCAAGAC	Mironov et al. 2012
ITS-2	ITS2_Eutar_Ff	forward	CGTAACGTGAATTGCAGGAC	Stec et al. 2018b	Stec et al.
	ITS2_Eutar_Rr	reverse	TGATATGCTTAAGTTCAGCGG	Stec et al. 2018b	2018b