A new species of freshwater Chaetonotidae (Gastrotricha, Chaetonotida) from Obodska Cave (Montenegro) based on morphological and molecular characters Author Kolicka, Małgorzata Department of Animal Taxonomy and Ecology, Institute of Environmental Biology, Adam Mickiewicz University Poznan, Umultowska 89, 61 - 614 Poznan, Poland. & Corresponding author: kolicka @ amu. edu. pl kolicka@amu.edu.pl Author Gadawski, Piotr Department of Invertebrate Zoology and Hydrobiology, University of Łódź, Banacha 12 / 16, 90 - 237 Łódź, Poland. & E-mail: gadawski @ biol. uni. lodz. pl Author Dabert, Miroslawa Molecular Biology Techniques Laboratory, Faculty of Biology, Adam Mickiewicz University Poznan, Umultowska 89, 61 - 614 Poznan, Poland. & E-mail: mirkad @ amu. edu. pl text European Journal of Taxonomy 2017 2017-09-28 354 1 30 journal article 21993 10.5852/ejt.2017.354 d10a5ca6-6a4f-4fd5-95a6-3874ecc413b6 2118-9773 3836285

Table 2. PCR primers used in this study; A and S refer to amplifying and sequencing, respectively.

Primer name Sequence (5’–3’)		Product Use	Source
bcdF08	CGATGRTTTTTTTCHACWAACCAYAARGATATCGG COI A		Kolicka et al. 2016
bcdF09	CGATGRTTTTTTTCHACWAACCAYAARGACATTGG COI A, S		Kolicka et al. 2016
bcdR04	TATAAACYTCDGGATGNCCAAAAAA	COI A, S	Dabert et al. 2008
ITS1_18S	AGAGGAAGTAAAAGTCGTAACAAG	ITS A, S	Navajas et al. 1998
ITS2_28S	ATATGCTTAAATTCAGGGGG	ITS A, S	Navajas et al. 1998
18Sfw	CTTGTCTCAAAGATTAAGCCATGCA	18S rDNA A, S	Dabert et al. 2010
rev480	GTTATTTTTCGTCACTACCT	18S rDNA S	Dabert et al. 2010
fw390	AATCAGGGTTCGATTCCGGAGA	18S rDNA A, S	Dabert et al. 2010
rev960	GACGGTCCAAGAATTTCAC	18S rDNA A, S	Dabert et al. 2010
for1300	TGCATGGCCGTTCTTAGTTG	18S rDNA S	Dabert et al. 2010
rev1460	CATCACAGACCTGTTATTGC	18S rDNA S	Dabert et al. 2010
rev18S	TGATCCTTCCGCAGGTTCACCT	18S rDNA A, S	Dabert et al. 2010
28SF0001	ACCCVCYNAATTTAAGCATAT	28S rDNA A	Dabert et al. 2016
1634LReverse ATTCGGCAGGTGAGTTGTTACA		28S rDNA S	Telford et al. 2003
1200F	CCCGAAAGATGGTGAACTATGC	28S rDNA S	Telford et al. 2003
2450R	GCTTTGTTTTAATTAGACAGTCGGA	28S rDNA A, S	Telford et al. 2003
28SR2850	GTGGTTTCGCTAGATAGTAGATA	28S rDNA A, S	Dabert et al. 2016
300F	CAAGTACCGTGAGGGAAAGTTG	28S rDNA S	Telford et al. 2003
300R	CAACTTTCCCTCACGGTACTTG	28S rDNA S	Telford et al. 2003
1200R	GCATAGTTCACCATCTTTCGG	28S rDNA S	Telford et al. 2003
UJR2176	CGGATCTAATTTGCCGACTTCCCTTA	28S rDNA S	Telford et al. 2003
1600F	AGCAGGACGGTGGCCATGGAAG	28S rDNA S	Telford et al. 2003