Identification and occurrence of phenylethanoid and iridoid glycosides in six Polish broomrapes (Orobanche spp. and Phelipanche spp., Orobanchaceae) Author Jedrejek, Dariusz Department of Biochemistry and Crop Quality, Institute of Soil Science and Plant Cultivation, State Research Institute, 24 - 100, Puławy, Poland Author Pawelec, Sylwia Author Piwowarczyk, Renata Author Pecio, Łukasz Author Stochmal, Anna text Phytochemistry 2020 112189 2020-02-29 170 1 12 http://dx.doi.org/10.1016/j.phytochem.2019.112189 journal article 10.1016/j.phytochem.2019.112189 1873-3700 8194197

Table 1 UHPLC-QTOF-MS/MS data of metabolites identified in the studied Polish broomrape species, and molar absorption coefficients (ε) of isolated phenylethanoid glycosides determined at 330 nm.

No	Identity	RT (min)	Formula	UVmax (nm)	Error (ppm)	mσ	Observed [M-H]-	Major fragments (%)	Molar absorption coefficient (ε) at 330 nm (m2·mol −1)
a Isolated and elucidated by NMR. b Novel compound.
1	Asperulosidic acid a	2.2	C18H24O12	235	2	10.7	431.1186	431.1186 (100); 251.0558 (66); 165.0555 (61); 225.0765 (19); 147.0441	not determined
2	Asperuloside a	3.4	C18H22O11	237	0.8	11.2	413.1083	(18) 147.0449 (100); 413.1083 (29); 191.0349 (20); 251.0556 (7); 233.0463 (3)	not determined
3 4 5 6 7 8 9	Echinacoside Arenarioside a Acteoside a Tubuloside A a Poliumoside a Isoacteoside a Crenatoside a	6.9 9.4 10.1 10.3 10.5 11.3 11.5	C35H46O20 C34H44O19 C29H36O15 C37H48O21 C35H46O19 C29H36O15 C29H34O15	290sh, 330 290sh, 330 290sh, 330 290sh, 330 290sh, 330 290sh, 328 290sh, 330	0.9 1.1 1 0.2 1.2 2 2.5	18 20.9 12 17.4 19.6 13.9 20.8	785.2502 755.2395 623.1975 827.2613 769.2551 623.1969 621.1809	161.0240 (100); 623.2190 (20); 133.0293 (13); 477.1606 (6); 179.0350 (6) 161.0242 (100); 593.2084 (21); 133.0296 (9); 755.2396 (5); 135.0449 (5) 161.0241 (100); 623.1975 (21); 461.1658 (20); 133.0294 (6); 135.0450 (4) 161.0241 (100); 623.2189 (21); 133.0294 (13); 477.1610 (5); 135.0450 (4) 161.0244 (100); 607.2237 (18); 133.0297 (10); 461.1661 (6); 769.2550 (6) 161.0241 (100); 623.1970 (41); 461.1657 (37); 179.0348 (6); 133.0294 (4) 161.0240 (100); 179.0345 (82); 621.1810 (18); 151.0399 (14); 135.0450	not determined 2186 ± 35 1766 ± 25 2099 ± 27 2084 ± 36 1250 ± 20 1757 ± 26
10	Ramoside A a,b	12.4	C35H46O18	317	2.5	31.2	753.2593	(13); 459.1502 (6) 145.0294 (100); 607.2234 (26); 117.0347 (12); 461.1661 (10); 153.0557	1785 ± 28
11	3- O -Methylpoliumoside a	12.5	C36H48O19	290sh, 330	3	29.6	783.2694	(10) 175.0397 (100); 160.0171 (33); 607.2233 (24); 193.0504 (14); 153.0555	1910 ± 27
12 13	Pheliposide a Leucosceptoside A a	12.8 12.8	C36H46O20 C30H38O15	290sh, 332 290sh, 330	1.1 1.8	20.7 26.8	797.2501 637.2126	(14) 161.0239 (100); 593.2079 (22); 133.0292 (12); 447.1502 (5); 135.0448 (4) 175.0399 (100); 461.1659 (35); 160.0178 (20); 193.0506 (18); 135.0450	2352 ± 33 not determined
14 15	2′- O -Acetylacteoside a 2′- O -Acetylpoliumoside a	13.8 14.0	C31H38O16 C37H48O20	290sh, 330 290sh, 330	2.2 1.5	20.9 10.8	665.2073 811.2654	(9) 161.0239 (100); 461.1656 (22); 133.0292 (8); 665.2073 (7); 503.1765 (4) 161.0244 (100); 607.2238 (18); 133.0297 (12); 461.1663 (6); 135.0453 (4)	1716 ± 26 2115 ± 35
16 17	2′- O -Acetylisoacteoside 2′- O -Acetylramoside A a,b	15.6 15.9	C31H38O16 C37H48O19	290sh, 328 317	2.2 2.8	27 16.2	665.2072 795.2695	161.0240 (100); 461.1657 (26); 665.2072 (11); 503.1759 (6); 133.0292 (6) 145.0299 (100); 607.2251 (26); 461.1670 (12); 117.0354 (10); 163.0407	not determined 1831 ± 31
								(6)
18	Wiedemannioside D a	15.9	C38H50O20	290sh, 330	−3.7	15.5	825.2853	175.0403 (100); 160.0176 (41); 607.2246 (29); 461.1667 (13); 193.0511	2108 ± 35
(10)