Ulva L. biodiversity in the central Mediterranean Sea: cryptic species and new records Author Bartolo, Angela G. School of Biological Sciences, University of Aberdeen, Cruickshank Building, St. Machar Drive, Aberdeen AB 24 3 UU, Scotland (United Kingdom) and Laboratory of Applied Phycology, Centre for Molecular Medicine & Biobanking, Fourth floor, Biomedical Sciences Building, University of Malta, Msida, MSD 2080 (Malta) angiebartolo @ gmail. com (corresponding author) Author Zammit, Gabrielle Laboratory of Applied Phycology, Centre for Molecular Medicine & Biobanking, Fourth floor, Biomedical Sciences Building, University of Malta, Msida, MSD 2080 (Malta) and Microbiology Lab, Department of Biology, Second floor, Biomedical Sciences Building, Faculty of Science, University of Malta, Msida, MSD 2080 (Malta) Author Küpper, Frithjof C. School of Biological Sciences, University of Aberdeen, Cruickshank Building, St. Machar Drive, Aberdeen AB 24 3 UU, Scotland (United Kingdom) and Marine Biodiscovery Centre, Department of Chemistry, University of Aberdeen, Aberdeen AB 24 3 UE, Scotland (United Kingdom) * Both these authors have contributed equally to this study. text Cryptogamie, Algologie 2022 2022-12-14 20 14 215 225 http://dx.doi.org/10.5252/cryptogamie-algologie2022v43a14 journal article 10.5252/cryptogamie-algologie2022v43a14 1776-0992 7819486

TABLE 3. — PCR programme conditions used for each primer pair in this study.

			Amplification
Primer pairs Initial denaturation		Cycles	Denaturation	Annealing	Elongation Final extension Reference
1 and 2	4 min at 94°C	38	1 min at 94°C	30 s at 45°C	1 min at 72°C	7 min at 72°C	Saunders & Kucera 2010
3 and 4	3 min at 95°C	35	15 s at 95°C	15 s at 45°C	1 min at 72°C	5 min at 72°C	Taylor et al. 2017
5 and 6	2 min at 95°C	35	1 min at 93°C	45 s at 50°C	2 min at 72°C	7 min at 72°C	Saunders & Kucera 2010