Integrative descriptions of two new Macrobiotus species (Tardigrada, Eutardigrada, Macrobiotidae) from Mississippi (USA) and Crete (Greece) Author Vecchi, Matteo https://orcid.org/0000-0002-7995-6827 Department of Biological and Environmental Science, University of Jyvaskyla, PO Box 35, FI- 40014 Jyvaeskylae, Finland matteo.m.vecchi@jyu.fi Author Stec, Daniel https://orcid.org/0000-0001-6876-0717 Department of Invertebrate Evolution, Institute of Zoology and Biomedical Research, Faculty of Biology, Jagiellonian University, Gronostajowa 9, 30 - 387 Krakow, Poland text Zoosystematics and Evolution 2021 2021-05-19 97 1 281 306 http://dx.doi.org/10.3897/zse.97.65280 journal article http://dx.doi.org/10.3897/zse.97.65280 1860-0743-1-281 BCA8CCABC5784FEBB053F483D53922AD D784822D3E055039B543B7FC864ADF5C
Table 1. Primers with their original references used for amplification of the four DNA fragments sequenced in the study.
DNA markerPrimer namePrimer directionPrimer sequence (5 '-3')Primer source
18S rRNA18S_Tar_Ff1forwardAGGCGAAACCGCGAATGGCTCStec et al. (2017a)
18S_Tar_Rr1reverseGCCGCAGGCTCCACTCCTGG
28S rRNA28S_Eutar_FforwardACCCGCTGAACTTAAGCATATGasiorek et al. (2018)
28SR0990reverseCCTTGGTCCGTGTTTCAAGACMironov et al. (2012)
ITS-2ITS2_Eutar_FfforwardCGTAACGTGAATTGCAGGACStec et al. (2018a)
ITS2_Eutar_RrreverseTCCTCCGCTTATTGATATGC
COILCO1490-JJforwardCHACWAAYCATAAAGATATYGGAstrin and Stueben (2008)
HCO2198-JJreverseAWACTTCVGGRTGVCCAAARAATCA