A mini-review: Potential of Phyllanthus niruri L as immunostimulators in fish aquaculture Author Rosidah Department of Fisheries, Faculty of Fishery and Marine Science, Universitas Padjadjaran, Bandung, Indonesia text International Journal of Fisheries and Aquatic Studies 2023 2023-01-01 11 1 26 30 http://dx.doi.org/10.22271/fish.2023.v11.i1a.2765 journal article 10.22271/fish.2023.v11.i1a.2765 04764dd2-e290-4bda-bb86-5e7070125152 2347-5129 11659163

Table 1: Applications of Phyllanthus niruri L. Sebagai Immunostimulant

Jenis Ikan	Treatment	Metode Uji Tantang	Hasil	Ref
Oreochromis mossambicus	Administration of P. niruri aqueous extract via intraperitoneal.	Injection Fish were injected intraperitoneally with Sheep red blood cells (SRBC). The first dose of 0.1 mL of 5% and the second dose (booster) of 0.1 mL of 25%	P. niruri extract dose of 20 mg/fish body weight resulted in a maximum increase in antibody levels on day 15.	[34]
Oreochromis mossambicus	Administration of P. niruri leaf aqueous extract by intraperitoneal injection.	Fish were injected intraperitoneally with HA-BSA (heat aggregated-bovine serum albumin) with a concentration of 5 mg as much as 100 μL.	A dose of P. niruri extract of 0.02 mg/kg body weight resulted in a maximum increase in neutrophil levels on the 6th day.	[34]
Oreochromis niloticus	Administration of P. niruri leaf aqueous extract by injection.	Infecting fish by injection with Aeromonas hydrophila bacteria.	The concentration of 50 mg significantly resulted in the highest survival, which was 76.7%.	[35]
Cyprinus carpio	Giving water extract of P. niruri leaves through feed.	Infection of fish with A. hydrophila 103 CFU in cohabitation through water media in rearing aquariums.	The concentration of 2% (10 g/500 g of feed) resulted in the highest fish survival, namely 100%.	[36]