Integrative systematics illuminates the relationships in two sponge-associated hydrozoan families (Capitata: Sphaerocorynidae and Zancleopsidae) Author Maggioni, Davide Author Schuchert, Peter Author Arrigoni, Roberto Author Hoeksema, Bert W. Author Huang, Danwei Author Strona, Giovanni Author Seveso, Davide Author Berumen, Michael L. Author Montalbetti, Enrico Author Collins, Richard Author Galli, Paolo Author Montano, Simone text Contributions to Zoology 2021 2021-11-02 90 487 525 http://dx.doi.org/10.1163/18759866-bja10023 journal article 268190 10.1163/18759866-BJA10023 d7f4a60b-8e7c-4354-8a01-1bae53791dd6 1875-9866 8343324

TABLE 2 Molecular diagnostic characters for each sphaerocorynid genus and molecular marker, except for ITS. Positions refer to reference sequences belonging to S. bedoti (MHNG-INVE-0137431), with the GenBank accession numbers MZ457614 (16S), MZ457396 (COX1), MZ463827 (COX3), MZ457880 (18S), and MZ457801 (28S). When positions in the alignments differ from reference sequences they are also reported in parentheses. If a diagnostic position corresponds to a position in which there is a gap in the reference sequence, it is only reported in parentheses

Genus /	Nr. of	Diagnostic position and relative nucleotide
molecular marker	diagnostic
positions
Sphaerocoryne
16S	16	157: C, 174: G, 180: C, 209: A, 264: T, 269: A, 273: G, 287: T, 356:
		C, 360: A, 374: A, 376: C, 394: A, 396: T, 406: A, 408: G
COX1	20	27: T, 30: T, 72: A, 129: A, 138: T, 177: C, 186: T, 271: C, 273: T,
		396: A, 402: A, 432: T, 466: A, 513: C, 528: T, 531: C, 570: T, 588:
		T, 591: C, 594: T
COX3	22	37: A, 55: C, 63: A, 109: G, 144: T, 145: A, 147: T, 150: G, 168:
		G, 271: G, 336: A, 357: A, 401: C, 408: A, 418: G, 429: A, 477:
		A, 480: T, 483: A, 563: G, 564: G, 573: G
18S	10	(75): -, 175 (177): C, 185 (187): T, 186 (188): T, 1297 (1300): G, 1298
		(1301): A, 1306 (1309): T, 1307 (1310): T, 1308 (1311): T, 1318
		(1321): T
28S	11	4: C, 32: T, 39: G, 43: C, 44: A, 45: T, 46: C, 93: T, 102: C, 103:
		T, 109: G
Kudacoryne
16S	12	50: A, 51: T, 52: A, 173: C, 186: T, 203: C, 216: C, 259: T, 285:
		A, 290: T, 294: A, 380: C
COX1	17	30: C, 33: G, 75: A, 84: C, 180: G, 189: A, 261: C, 291: T, 348:
		A, 381: C, 402: C, 411: A, 432: C, 483: T, 516: C, 564: G, 597: T
COX3	14	15: A, 36: G, 61: C, 99: T, 183: A, 216: C, 237: C, 271: T, 276:
		A, 306: A, 345: T, 402: A, 417: T, 591: C
18S	1	227 (230): A
28S	1	439 (440): C
Euphysilla
16S	5	184: G, 275: G, 287: -, 360: T, 434: G
COX1	9	168: T, 243: T, 274: C, 288: A, 289: G, 312: T, 339: T, 546: T, 603: T
COX3	4	79: G, 213: G, 255: T, 387: A
18S	2	1420 (1423): T, 1476 (1479): A
28S	0	-
Heterocoryne
16S	21	53: C, 63: A, 116: C, 168: T, 178: G, 194: G, 208: C, 219: A, 276: G,
		278: T, 280: A, 286: T, 298: G, 299: T, 331: C, 333: T, 344: T, 352:
		A, 354: T, 356: A, 379: C