Improved diagnostic sensitivity of human strongyloidiasis using point-of-care mixed recombinant antigen-based immunochromatography Author Boonroumkaew, Patcharaporn Department of Parasitology, Faculty of Medicine, Khon Kaen University, 40002 Khon Kaen, Thailand & Mekong Health Science Research Institute, Khon Kaen University, 40002 Khon Kaen, Thailand Author Sadaow, Lakkhana Department of Parasitology, Faculty of Medicine, Khon Kaen University, 40002 Khon Kaen, Thailand & Mekong Health Science Research Institute, Khon Kaen University, 40002 Khon Kaen, Thailand Author Janwan, Penchom Mekong Health Science Research Institute, Khon Kaen University, 40002 Khon Kaen, Thailand & Department of Medical Technology, School of Allied Health Sciences, Walailak University, 80161 Nakhon Si Thammarat, Thailand & Hematology and Transfusion Science Research Center, Walailak University, 80161 Nakhon Si Thammarat, Thailand Author Rodpai, Rutchanee Department of Parasitology, Faculty of Medicine, Khon Kaen University, 40002 Khon Kaen, Thailand & Mekong Health Science Research Institute, Khon Kaen University, 40002 Khon Kaen, Thailand Author Sanpool, Oranuch Department of Parasitology, Faculty of Medicine, Khon Kaen University, 40002 Khon Kaen, Thailand & Mekong Health Science Research Institute, Khon Kaen University, 40002 Khon Kaen, Thailand Author Buadee, Punyisa Department of Parasitology, Faculty of Medicine, Khon Kaen University, 40002 Khon Kaen, Thailand & Mekong Health Science Research Institute, Khon Kaen University, 40002 Khon Kaen, Thailand Author Suprom, Chanida Department of Parasitology, Faculty of Medicine, Khon Kaen University, 40002 Khon Kaen, Thailand & Mekong Health Science Research Institute, Khon Kaen University, 40002 Khon Kaen, Thailand Author Thanchomnang, Tongjit Mekong Health Science Research Institute, Khon Kaen University, 40002 Khon Kaen, Thailand & Faculty of Medicine, Mahasarakham University, 44000 Maha Sarakham, Thailand Author Intapan, Pewpan M. Department of Parasitology, Faculty of Medicine, Khon Kaen University, 40002 Khon Kaen, Thailand & Mekong Health Science Research Institute, Khon Kaen University, 40002 Khon Kaen, Thailand Author Maleewong, Wanchai Department of Parasitology, Faculty of Medicine, Khon Kaen University, 40002 Khon Kaen, Thailand & Mekong Health Science Research Institute, Khon Kaen University, 40002 Khon Kaen, Thailand text Parasite 2023 Paris, France 2023-12-13 30 60 1 12 http://dx.doi.org/10.1051/parasite/2023063 journal article 299946 10.1051/parasite/2023063 1c47b38b-4350-4042-aa32-d932c32cd64e 1776-1042 PMC10723528 38099622 12524553

Table 1. Results of recombinant antigen-based immunochromatography tests (NIE, SsIR, and NIE-SsIR ICT kits) for detection of antiStrongyloides IgG antibody in human serum samples.

Conditions represented by sera	No. of sera	NIE ICT kit	SsIR ICT kit	NIE-SsIR ICT kit
1 CI, confidence interval. 2 The results were correlated between interpretations by the naked eye and the in-house strip reader.
	tested	No. positive2	No. positive2	No. positive2
Group I
Healthy controls	40	0	0	0
Group II
Proven strongyloidiasis	100	81	89	95
Group III
Giardiasis	7	1	2	2
Amoebiasis	10	1	3	0
Blastocystosis	10	2	4	3
Opisthorchiasis	10	5	2	0
Fascioliasis	10	3	1	3
Paragonimiasis	10	2	1	0
Taeniasis	10	2	3	1
Cysticercosis	10	3	2	2
Sparganosis	7	3	2	3
Hookworm infections	10	4	4	1
Ascariasis	10	0	0	0
Trichuriasis	10	2	0	0
Trichinellosis	10	0	2	1
Angiostrongyliasis	10	1	3	2
Gnathostomiasis	10	0	2	0
Capillariasis	10	2	1	1
			Diagnostic values [95% CI1]
Sensitivity (%)		81.0 [71.9–88.2]	89.0 [81.2–94.4]	95.0 [88.7–98.4]
Specificity (%)		84.0 [78.1–88.9]	83.5 [77.5–88.4]	90.2 [85.1–94.0]
Positive likelihood ratio		5.07 [3.62–7.10]	5.40 [3.90–7.46]	9.70 [6.31–14.90]
Negative likelihood ratio		0.23 [0.15–0.34]	0.13 [0.08–0.23]	0.06 [0.02–0.13]