Flavonoid biosynthesis in Dianthus caryophyllus L. is early regulated during interaction with Fusarium oxysporum f. sp. dianthi Author Romero-Rinc, Ana Author on * & Laboratory Research in Vegetal Metabolic Activities, Department of Chemistry, Faculty of Science, Universidad Nacional de Colombia, Ciudad Universitaria, Cra 30 No. & Bioorganic Chemistry Laboratory, Department of Chemistry, Universidad Militar Nueva Granada, Cajic´a, 250247, Colombia Author Martínez, Sixta Tulia Author Higuera, Blanca Ligia Author Coy-Barrera, Ericsson Author Ardila, Harold Duban text Phytochemistry 2021 112933 2021-12-31 192 1 14 http://dx.doi.org/10.1016/j.phytochem.2021.112933 journal article 10.1016/j.phytochem.2021.112933 1873-3700 8257971
Table 1 Annotation of top-ranked flavonoid-related features, selected from statistical analyses (PCA, MEBA, PLS-DA) that influenced discrimination between carnation cultivars (constitutive) and Fod -inoculated and non-inoculated carnation plants (induced).
#Rt[M-H] - (m/UV λ max (nm)Annotated metabolite (Flavonoid) aMolecularErrorMain fragments (m/z) c
a Annotation at level 3 of MSI confidence (Schymanski et al., 2014) by diagnostic evidence from UV, accurate mass and MS fragments data; Sugar residues: glc = glucose, ara = arabinose, rha = rhamnose, glu = glucuronide. b Molecular formula of the annotated compound [M], deduced from the adduct [M-H] -. c Diagnostic fragments observed in the respective HRMS spectra. d Annotated compound at level 4 of MSI confidence by HRMS, whose flavonoid type was deduced from UV spectrum data. PCA = principal component analysis; MEBA = multivariate empirical bayes approach; PLS-DA = partial-least squares-discriminant analysis.
(min)z)formula b(ppm)
161.3745.1624214, 228, 273,kaempferol 3- O -(galloyl-rha)-glcC34H34O191.07599.1048, 593.1517, 445.0782,
345285.0391
242.4579.1371214, 232, 279,kempferol 3- O -ara-glcC26H28O153.63417.0829, 293.0877, 285.0395
339
364.2801.2053214, 232, 282,methylquecetin 7- O -glc 3- O -glc-glcC34H42O224.49639.1569, 477.1042, 353.0965,
343315.0512
458.1515.1167215, 239, 275,kaempferol 3- O -diacetyl-rhaC25H24O124.27455.0955, 285.0406, 229.2069
341
561.8583.1073215, 236, 275,quercetin 3- O -(hydroybenzoyl-glc)C28H24O144.28455.0762, 301.0332, 281.0675
346
663.1797.2114214, 232, 283,kaempferol 3- O -(acetyl-rha)-glc-glcC35H42O213.26473.1069, 323.0965, 285.0388
343
743.1595.1313214, 232, 281,quercetin 3- O -ara-glcC26H28O162.35433.0776, 301.0351, 293.0885
346
854.9819.1982214, 232, 278,quercetin 3- O -(diacetyl-rha)-(triacetyl-C37H40O210.12547.1075, 517.1566, 301.0337
342glc)
958.3871.2131214, 233, 286,kaempferol 3- O -(malyl-rha)-glc-glcC37H44O241.49585.1653, 547.1096, 323.0989,
351285.0389
1044.6813.1531214, 232, 273,trihydroxyflavone O -(feruloyl-glu)-gluC37H34O212.09637.1182, 619.0935, 527.1045,
315285.0409,
1152.5815.2022214, 233, 277,kaempferol 3- O -(sinapoyl-glc)-glcC38H40O201.47653.1521, 529.1569, 591.1362,
348285.0380
1262.2717.2779215, 234, 278,flavonol d (+ rha-rha)C36H46O152.88571.2161, 425.1611, 291.1080
339
1354.4741.1639214, 233, 276,quercetin 3- O -(coumaroyl-ara)-glcC35H34O183.64577.1179, 439.1225, 433.0784,
348301.0335
1447.9559.1447217, 232, 275,hydroxydimethoxyflavone O -(diacetyl-C27H28O130.89313.0721, 245.0674
312glc)
1548.4757.1804214, 232, 278,quercetin 3- O -ara-glc-glcC32H38O213.04595.1287, 455.1414, 433.0764,
348301.0333
1648.2505.0983214, 232, 272,quercetin 3- O -(acetyl-glc)C23H22O130.20301.0355, 203.0562
348