How is the activity of shikimate dehydrogenase from the root of Petroselinum crispum (parsley) regulated and which side reactions are catalyzed? Author Hýskova, Veronika Department of Biochemistry, Faculty of Science, Charles University, Hlavova 2030, Prague 2, 128 40, Czech Republic Author Belonozníkov, Katerina Department of Biochemistry, Faculty of Science, Charles University, Hlavova 2030, Prague 2, 128 40, Czech Republic Author Smeringaiova, Ingrida Department of Biochemistry, Faculty of Science, Charles University, Hlavova 2030, Prague 2, 128 40, Czech Republic Author Kavan, Daniel Department of Biochemistry, Faculty of Science, Charles University, Hlavova 2030, Prague 2, 128 40, Czech Republic Author Ingr, Marek Department of Biochemistry, Faculty of Science, Charles University, Hlavova 2030, Prague 2, 128 40, Czech Republic & Tomas Bata University in Zlín, Faculty of Technology, Department of Physics and Materials Engineering, N´am. T. G. Masaryka 5555, 760 01, Zlín, Czech Republic & Tomas Bata University in Zlín, Faculty of Technology, Department of Physics and Materials Engineering, N´am. T. G. Masaryka 5555, 760 01, Zlín, Czech Republic Author Ryslava, Helena Department of Biochemistry, Faculty of Science, Charles University, Hlavova 2030, Prague 2, 128 40, Czech Republic text Phytochemistry 2021 112881 2021-10-31 190 1 12 http://dx.doi.org/10.1016/j.phytochem.2021.112881 journal article 53925 10.1016/j.phytochem.2021.112881 2ea59e53-b059-41a5-b5b5-30cb32d3aaf0 1873-3700 8270251

Table 4 The effect of various ions on P. crispum SDH activity. Screening was performed spectrophotometrically at 340 nm in the presence of 3 mM SA, 0.2 mM NADP and 5 mM*, 0.1 mM**, or 0.01 mM*** concentration of potential modulator. Each determination was done at least 3-times, the average values and S.D. are shown.

Compound	% of activity
(NH4)2SO4	115.6 ± 9.8 *
CaCl2	113.2 ± 8.2 *
NaCl	105.2 ± 3.7 *
KCl	107.0 ± 7.3 *
MgCl2	99.3 ± 15.0 *
MnCl2	77.6 ± 23.1 *
ZnSO4	10.1 ± 4.8 **
CuSO4	21.2 ± 8.3 ***