Systematics of the Stripetail Darter, Etheostoma kennicotti (Putnam), and the Distinctiveness of the Upper Cumberland Endemic Etheostoma cumberlandicum Jordan and Swain Author Near, Thomas J. Author Simmons, Jeffrey W. Author Strange, Rex M. Author Brandt, Stephanie Author Thomas, Matthew R. Author Harrington, Richard C. Author MacGuigan, Daniel J. text Ichthyology & Herpetology 2023 2023-04-18 111 2 203 221 http://dx.doi.org/10.1643/i2021053 journal article 302900 10.1643/i2021053 fc1c79b6-6a91-4df6-b235-7260123dc2e2 2766-1520 13744528

Table 2. Microsatellite primer sequences used in genotyping specimens of Etheostoma kennicotti and E. cumberlandicum.

Locus	Motif	Locus size in base pairs (bp)	50 primer	30 primer
Table 2.
Microsatellite primer sequences used in genotyping specimens of Etheostoma kennicotti and E. cumberlandicum.
Eken3	AC	168–218	TCCCAGTCACGACTGTGTGAGGCTGC	ACACAATCATCACTGCAGGTC
Eken8	AC	137–161	TCCCAGTCACGACACACACGTCTCAG	GGTGTTCTACTCTCCGTCCC
Eken10	AC	147–167	TCCCAGTCACGACCCATAACCCACTC	GCCGTAGCCTCTGTAATTGG
Eken12	AC	162–170	TCCCAGTCACGACCTATGTGTTCCGC	ACAAATGTTTCGGGCGCTG
Eken14	AC	152–178	TCCCAGTCACGACGCAGTTGTGTCAG	TGAGCAGGGTCTTATCCAGC
Eken15	AC	150–204	TCCCAGTCACGACCGTGACAAACAAG	GCAAATGGTGAACGGGTGTG
Eken18	AC	152–202	TCCCAGTCACGACCCCTTTCACCAAC	ATCATAGTGCAACAGGAGAGTC
Eken19	AG	136–186	TCCCAGTCACGACTGTGATATGATGC	AGACAGACCTGAAGCAGCAC
Eken21	AC	157–165	TCCCAGTCACGACAATAGAGTCCCTG	TGTTCATAGTGTCTGTGGCC
Eken22	AC	147–211	TCCCAGTCACGACTCCCATTGTGCAG	ACTATTGTTGAGAGCCGGTG
Eken24	AC	161–233	TCCCAGTCACGACTTGGTCTGAAGGG	TTTCTACGGCACTCCTGGG
Eken27	AC	157–219	TCCCAGTCACGACCTGTTTGAGAGTG	TTACCTTGCCTTTACACAACCC
Eken28	AC	152–190	TCCCAGTCACGACTCTAACGAACAGC	ACAGTAGCCCACACAGAAGG
Eken29	AG	155–223	TCCCAGTCACGACTGCGTTTGTCTCG	GAGCCGCCATCTCCAACAG
Eken32	AG	147–175	TCCCAGTCACGACTGTATGCTGGCAC	GGGATGATTAGTTTGTTGGTGG
Eken36	AC	153–177	TCCCAGTCACGACTGCTCCATTGCTC	TTCAGTGATCCCTGAAAGCG
Eken38	AG	150–168	TCCCAGTCACGACTCTTCATGGACGC	TGTGTGCATCTCTGTCCAGG
Eken39	AC	141–171	TCCCAGTCACGACTCACATGACGAGG	CACACCCGCATTGCAAACC
Eken41	AG	167–219	TCCCAGTCACGACATCAGAGTGACCC	CTTTCTCACAAACTCAGTTGCC
Eken42	AC	161–167	TCCCAGTCACGACCTGTAAGACAAAC	CGATTCACCTTTACACAACCTC
Eken49	AC	162–172	TCCCAGTCACGACCCATAAGACCGTG	TTCGCCTGCATTGTTCTTGG
Eken52	AG	140–228	TCCCAGTCACGACCCTGCCAATAACC	TATTCGACTACAGCCAGCGG
Eken58	AC	163–213	TCCCAGTCACGACCTTCCTTTCCACG	CAACCTGCACCACCCTTCTG
Eken59	AC	158–164	TCCCAGTCACGACCTTCCGCTCTCGC	CTGGGATCAACACTCTCTGC
Eken60	AC	155–163	TCCCAGTCACGACTCTGGTGGCTGGC	CCTCCCTCTCACTGCCAAC