Systematic assessment of the brown tree frog (Anura: Pelodryadidae: Litoria ewingii) reveals two endemic species in South Australia Author Parkin, Tom Australian Museum Research Institute, Australian Museum, 1 William St, Sydney, NSW 2010, Australia Author Rowley, Jodi J. L. Australian Museum Research Institute, Australian Museum, 1 William St, Sydney, NSW 2010, Australia & Centre for Ecosystem Science, School of Biological, Earth and Environmental Sciences, University of New South Wales, Sydney, NSW 2052, Australia Author Elliott-Tate, Jessica Australian Museum Research Institute, Australian Museum, 1 William St, Sydney, NSW 2010, Australia & Centre for Ecosystem Science, School of Biological, Earth and Environmental Sciences, University of New South Wales, Sydney, NSW 2052, Australia Author Mahony, Michael J. School of Environmental and Life Sciences, University of Newcastle, University Drive, Callaghan, NSW 2308, Australia Author Sumner, Joanna Museums Victoria Research Institute, Melbourne Museum, GPO Box 666, Melbourne, VIC 3001, Australia Author Melville, Jane Museums Victoria Research Institute, Melbourne Museum, GPO Box 666, Melbourne, VIC 3001, Australia & School of Biological Sciences, Monash University, Clayton, VIC 3168 Australia Author Donnellan, Stephen C. South Australian Museum, North Terrace, Adelaide, SA 5000, Australia text Zootaxa 2024 2024-02-02 5406 1 1 36 https://www.mapress.com/zt/article/download/zootaxa.5406.1.1/52824 journal article 287337 10.11646/zootaxa.5406.1.1 b8a43eaa-2492-4e47-b82a-0815be55f4e8 1175-5326 10610157 10765250-45BD-4988-A91C-158BE19F9E55

TABLE 1. Litoria ewingii Group specimens examined for molecular genetic analyses. Institution codes: ABTC —Australian Biological Tissue Collection, South Australian Museum; NMV—Museums Victoria; AMS—Australian Museum; SAMA—South Australian Museum; QM—Queensland Museum; ANWC—Australian National Wildlife Collection;TMAG—Tasmanian Museum and Art Gallery.State abbreviations: SA—South Australia; Vic—Victoria; NSW—New South Wales; Tas—Tasmania; Qld—Queensland; ACT—Australian Capital Territory. ND4 column refers to GenBank accession numbers.

Tissue RegNum	Species	ND4	SNP	Locality	State	Latitude	Longitude	Voucher institution	Voucher RegNum
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ABTC 14683	Litoria calliscelis	OR546026	y	Highbury	SA	-34.830	138.680	SAMA	R34901
ABTC 33268	Litoria calliscelis	OR546027	-	22 km ESE Mt Compass	SA	-35.428	138.809	SAMA	R49211
ABTC 74464	Litoria calliscelis	OR546028	-	1.5 km NE Norton Summit	SA	-34.912	138.738	SAMA	R54982
ABTC 93424	Litoria calliscelis	-	y	1.8 km NNE Caloote	SA	-34.951	139.272	SAMA	R59158
ABTC 93463	Litoria calliscelis	-	y	4.6 km ENE Pellaring Flat	SA	-34.859	139.442	SAMA	R59174
ABTC 94832	Litoria calliscelis	OR546029	y	0.7 km NE Pijanbilli Lodge	SA	-35.546	138.374	SAMA	R53569
ABTC 94968	Litoria calliscelis	-	y	Wangoola Homestead	SA	-35.566	138.367	SAMA	R53645
ABTC 12434	Litoria ewingii	OR546010	y	3 km E Toora	Vic	-38.670	146.370	SAMA	R39003
ABTC 12435	Litoria ewingii	OR546012	-	3 km E Toora	Vic	-38.670	146.370	SAMA	R39004
ABTC 12436	Litoria ewingii	MT497817	-	3 km E Toora	Vic	-38.670	146.370	SAMA	R39005
ABTC 12437	Litoria ewingii	OR546013	-	3 km E Toora	Vic	-38.670	146.370	SAMA	R39006
ABTC 132072	Litoria ewingii	OR546001	y	Halls Gap	Vic	-37.173	142.545	SAMA	R61927
ABTC 132074	Litoria ewingii	-	y	15km E Mt Gambier	Vic	-37.814	141.001	SAMA	R61929
ABTC 132075	Litoria ewingii	OR546004	y	15 km E Mt Gambier	Vic	-37.814	141.001	SAMA	R61930
ABTC 142838	Litoria ewingii	-	y	Ferndale, Strzelecki Ranges	Vic	-38.310	145.971	SAMA	R62478
ABTC 14721	Litoria ewingii	OR545997	y	Penola State Forest	SA	-37.470	140.830	SAMA	R34908
ABTC 14736	Litoria ewingii	OR545993	y	Penola	SA	-37.380	140.830	SAMA	R34913
ABTC 16984	Litoria ewingii	OR545999	y	near Bessie Belle	Vic	-38.170	141.970	SAMA	R35813
ABTC 17602	Litoria ewingii	OR546015	y	Boydtown	NSW	-37.100	149.880	SAMA	R42612
ABTC 17605	Litoria ewingii	OR546014	y	4 km S Narrabarba	NSW	-37.270	149.800	SAMA	R42615
ABTC 23116	Litoria ewingii	OR546020	-	Pigsty Ponds	Tas	-43.487	146.727	TMAG	C872
ABTC 37513	Litoria ewingii	-	y	17.3 km WNW Kongorong Forest Reserve	SA	-37.902	140.393	SAMA	R49566
ABTC 37521	Litoria ewingii	OR545994	-	0.5 km E Carpenters Rocks	SA	-37.911	140.400	SAMA	R49578
ABTC 37533	Litoria ewingii	MT497818	y	20 km WNW Millicent Airport	SA	-37.507	140.149	SAMA	R49588
ABTC 58288	Litoria ewingii	OR545995	y	4.3 km E Dunmore Homestead	SA	-36.236	140.068	SAMA	R45982
ABTC 58652	Litoria ewingii	OR546002	y	Tarpeena	SA	-37.699	140.798	SAMA	R49330
ABTC 70043	Litoria ewingii	OR545996	y	9 km SW Millicent	SA	-37.633	140.275	SAMA	R53776
ABTC 70616	Litoria ewingii	y	y	4.6 km SSW Bald Hill	SA	-36.553	140.004	SAMA	R53370
ABTC 7237	Litoria ewingii	OR545992	-	Lucindale	SA	-36.970	140.370
ABTC 94804	Litoria ewingii	OR545998	y	7.7 km SE Kangaroo Hill	SA	-37.206	139.928	SAMA	R53767
EBU87903	Litoria ewingii	OR546007	y	Jancourt Nature Conservation Reserve	Vic	-38.391	143.191
JJLR5379	Litoria ewingii	OR546023	y	Gladstone	Tas	-40.936	148.087	TMAG	C1527
JJLR5380	Litoria ewingii	-	y	Gladstone	Tas	-40.936	148.087	TMAG	C1528
JJLR5381	Litoria ewingii	OR546022	y	Pioneer	Tas	-41.071	147.925	TMAG	C1529
JJLR5383	Litoria ewingii	OR546024	y	Penguin	Tas	-41.140	146.062	TMAG	C1530
JJLR5389	Litoria ewingii	OR546025	y	E of Temma	Tas	-41.215	144.902	TMAG	C1531
JJLR5393	Litoria ewingii	-	y	Zeehan	Tas	-41.819	145.125	TMAG	C1532
JJLR5394	Litoria ewingii	-	y	Zeehan	Tas	-41.819	145.167	TMAG	C1533
JJLR5396	Litoria ewingii	OR546021	y	~15 km W Eaglehawk Neck Tasman Peninsula	Tas	-43.038	147.747	TMAG	C1534
JJLR5398	Litoria ewingii	-	y	Bicheno	Tas	-41.930	148.228	TMAG	C1535
JJLR5894	Litoria ewingii	OR546016	y	Colquhoun/Boyanga Gidi State Forest	Vic	-37.743	148.044	NMV	D 76455
JJLR5895	Litoria ewingii	y	y	Colquhoun/Boyanga Gidi State Forest	Vic	-37.743	148.044	NMV	D 76456
JJLR5901	Litoria ewingii	OR546011	y	Bunyip State Forest	Vic	-37.957	145.915	NMV	D 76457
JJLR5902	Litoria ewingii	OR546018	y	Bunjil Bushland Reserve	Vic	-37.647	145.246	NMV	D 76458
JJLR5908	Litoria ewingii	OR546006	y	Ross Creek State Forest	Vic	-37.668	143.697	NMV	D 76459
JJLR5909	Litoria ewingii	OR546003	y	Mount Clay State Forest	Vic	-38.226	141.686	NMV	D 76460
JJLR5910	Litoria ewingii	OR546000	y	Mount Clay State Forest	Vic	-38.192	141.680	NMV	D 76461
JJLR5913	Litoria ewingii	OR546005	y	Halls Gap	Vic	-37.171	142.542	NMV	D 76462
NMVZ 11155	Litoria ewingii	-	y	Ming Ming Swamp, Grampians	Vic	-37.331	142.193	NMV	D 75485
NMVZ 12831	Litoria ewingii	OR546019	y	Mount Disappointment	Vic	-37.377	145.096
NMVZ 12832	Litoria ewingii	-	y	Heathcote Junction	Vic	-37.377	145.096
NMVZ 12833	Litoria ewingii	y	y	Mount Disappointment	Vic	-37.377	145.096
NMVZ 12834	Litoria ewingii	-	y	Heathcote Junction	Vic	-37.377	145.096
NMVZ 12835	Litoria ewingii	-	y	Heathcote Junction	Vic	-37.377	145.096
NMVZ 13203	Litoria ewingii	KC183742	-	Castella	Vic	-37.517	145.420
NMVZ 13204	Litoria ewingii	KC183741	-	Castella	Vic	-37.517	145.420
NMVZ 13205	Litoria ewingii	-	y	Pakenham	Vic	-38.032	145.433
NMVZ 13206	Litoria ewingii	-	y	Pakenham	Vic	-38.032	145.433
NMVZ 13208	Litoria ewingii	-	y	Pakenham	Vic	-38.032	145.433
NMVZ 13222	Litoria ewingii	KC183740	-	Castella	Vic	-37.517	145.420
NMVZ 13223	Litoria ewingii	KC183733	-	Castella	Vic	-37.517	145.420
NMVZ 13375	Litoria ewingii	KC183735	-	Yarra Valley	Vic	-37.562	145.417
NMVZ 27038	Litoria ewingii	-	y	Castella	Vic	-37.504	145.405
NMVZ 34908	Litoria ewingii	-	y	Dowd Morass Wildlife Reserve	Vic	-38.145	147.146
NMVZ 60370	Litoria ewingii	-	y	Wombat Creek	Vic	-37.771	148.243	NMV	D 77301
NMVZ 60376	Litoria ewingii	-	y	Wombat Creek	Vic	-37.772	148.245	NMV	D 77306
NMVZ 60387	Litoria ewingii	-	y	Wombat Creek	Vic	-37.771	148.243	NMV	D 77315
NMVZ 60403	Litoria ewingii	-	y	Providence Ponds Nature Conservation Reserve	Vic	-37.934	147.294	NMV	D 77809
NMVZ 60617	Litoria ewingii	OR546008	-	Anglesea Heath	Vic	-38.349	144.136	NMV	D 77211
NMVZ 60627	Litoria ewingii	-	y	Anglesea Heath	Vic	-38.402	144.145	NMV	D 77223
NMVZ 60629	Litoria ewingii	OR546009	y	Anglesea	Vic	-38.402	144.145	NMV	D 77221
NMVZ 60630	Litoria ewingii	y	y	Anglesea	Vic	-38.402	144.145	NMV	D 77220
NMVZ 60633	Litoria ewingii	-	y	Anglesea Heath	Vic	-38.402	144.145	NMV	D 77218
ABTC 13646	Litoria ewingii (“northern”)	y	y	Blowering	NSW	-35.470	148.280
ABTC 13700	Litoria ewingii (“northern”)	OR546041	y	Tumbarumba	NSW	-35.850	148.120
AMSR 140834	Litoria ewingii (“northern”)	-	y	Bogong Creek, Kosciusko National Park	NSW	-36.250	148.250	AMS	AMSR 140834
AMSR 188372	Litoria ewingii (“northern”)	-	y	Tumbarumba	NSW	-35.783	147.986	AMS	AMSR 188372
AMSR 188373	Litoria ewingii (“northern”)	-	y	Tumbarumba	NSW	-35.783	147.986	AMS	AMSR 188373
ABTC 25157	Litoria jervisiensis	y	y	Awabakal Nature Reserve, Dudley	NSW	-33.000	151.720
ABTC 25158	Litoria jervisiensis	y	y	Awabakal Nature Reserve, Dudley	NSW	-33.000	151.720
ABTC 25451	Litoria jervisiensis	MT497819	y	Darkes Forest	NSW	-34.220	150.900
ABTC 25839	Litoria jervisiensis	MT497820	y	Mungo Brush, Myall Lakes National Park	NSW	-32.500	152.300
ABTC 80824	Litoria jervisiensis	y	y	Kioloa	NSW	-35.550	150.370
ABTC 90506	Litoria jervisiensis	y	y	Kurnell Peninsula	NSW	-34.026	151.218
ABTC 145264	Litoria littlejohni	MT497842	-	Kings Tableland	NSW	-33.814	150.438
ABTC 150907	Litoria littlejohni	MT497840	-	Andersons Fire Trail, Kings Plateau	NSW	-33.803	150.426
ABTC 150908	Litoria littlejohni	MT497841	-	Andersons Fire Trail, Kings Plateau	NSW	-33.803	150.426
ABTC 97410	Litoria littlejohni	MT497833	-	Olney State Forest	NSW	-33.098	151.350
ABTC 97411	Litoria littlejohni	MT497831	-	Olney State Forest	NSW	-33.067	151.339
ABTC 12855	Litoria paraewingi	MT497821	y	7 km N Merton	Vic	-36.930	145.750	SAMA	R44074
ABTC 12856	Litoria paraewingi	MT497822	y	6 km W Strathbogie	Vic	-36.880	145.680	SAMA	R44066
ABTC 17553	Litoria paraewingi	-	y	East Albury	NSW	-36.080	146.930	SAMA	R42148
ABTC 17554	Litoria paraewingi	-	y	East Albury	NSW	-36.080	146.930	SAMA	R42149
ABTC 40923	Litoria paraewingi	MT497823	-	Reef Hills State Park	Vic	-36.600	145.950	SAMA	R34675
NMVZ 13521	Litoria paraewingi	-	y	Murrindindi	Vic	-37.383	145.482
NMVZ 13541	Litoria paraewingi	-	y	Glenburn	Vic	-37.432	145.459
NMVZ 13608	Litoria paraewingi	OR546040	y	Beechworth	Vic	-36.358	146.700
NMVZ 26990	Litoria paraewingi	-	y	Marysville	Vic	-37.492	145.748
ABTC 25078	Litoria revelata	OR545978	-	Wild Cattle Creek State Forest	NSW
ABTC 25996	Litoria revelata	OR545979	-	Coolongolook State Forest	NSW	-32.217	152.317
ABTC 26010	Litoria revelata	OR545980	-	Pine Creek State Forest	NSW
ABTC 80826	Litoria revelata	OR545981	-	Maxwell Flat	NSW	-31.480	152.180	SAMA	R68987
ABTC 86373	Litoria revelata	OR545982	-	Nightcap Range, Whian Whian State Forest	NSW	-28.600	153.377	ANWC	A01997
ABTC 102390	Litoria revelata (north Qld)	MT497815	-	Zillie Falls Road, Ellinjaa	Qld	-17.467	145.649	QM	J90806
ABTC 102391	Litoria revelata (north Qld)	MT497816	-	Zillie Falls Road, Ellinjaa	Qld	-17.467	145.649
ABTC 80815	Litoria revelata (north Qld)	OR545983	-	Millaa Millaa	Qld	-17.490	145.610
ABTC 80816	Litoria revelata (north Qld)	OR545984	-	Millaa Millaa	Qld	-17.490	145.610
ABTC 33479	Litoria sibilus sp. nov.	OR545990	y	1.5 km NW Rocky River, Flinders Chase National Park	SA	-35.940	136.729	SAMA	R37403
ABTC 33511	Litoria sibilus sp. nov.	OR545991	y	Larrikin Lagoon, Flinders Chase National Park	SA	-35.835	136.687	SAMA	R37476
ABTC 7188	Litoria sibilus sp. nov.	OR545985	y	South West River, Kangaroo Island	SA	-35.980	136.870
ABTC 7189	Litoria sibilus sp. nov.	OR545986	y	South West River, Kangaroo Island	SA	-35.980	136.870	SAMA	R20183
ABTC 7190	Litoria sibilus sp. nov.	OR545989	y	South West River, Kangaroo Island	SA	-35.980	136.870	SAMA	R20184
ABTC 7192	Litoria sibilus sp. nov.	OR545987	y	South West River, Kangaroo Island	SA	-35.980	136.870	SAMA	R20186
ABTC 7193	Litoria sibilus sp. nov.	OR545988	y	South West River, Kangaroo Island	SA	-35.980	136.870	SAMA	R20187
ABTC 113113	Litoria verreauxii	y	-	Bairnsdale	Vic	-37.882	147.576
ABTC 113122	Litoria verreauxii	y	y	Macleods Morass	Vic	-37.837	147.623
ABTC 113125	Litoria verreauxii	-	y	Reedy Creek	Vic	-37.057	147.327
ABTC 12630	Litoria verreauxii	MT497828	y	Camden	NSW	-34.050	150.670	SAMA	R40839
ABTC 12644	Litoria verreauxii	OR546030	y	Paddy’s River crossing, Penrose State Forest	NSW	-34.650	150.130	SAMA	R40862
ABTC 13639	Litoria verreauxii	OR546035	y	Tantangara Dam	NSW	-35.800	148.670
ABTC 13650	Litoria verreauxii	OR546031	y	Bungendore	NSW	-35.300	149.450
ABTC 13661	Litoria verreauxii	OR546017	y	Sassafras	NSW	-35.330	150.520
ABTC 13668	Litoria verreauxii	OR546032	y	Mulligans Flats	ACT	-35.150	149.150
ABTC 13674	Litoria verreauxii	OR546036	-	Numeralla Creek	NSW	-36.080	149.130
ABTC 17451	Litoria verreauxii	OR546037	y	Little Thedbo River, Thredbo Valley	NSW	-36.500	148.420	SAMA	R40219
ABTC 17607	Litoria verreauxii	OR546038	y	7 km W Dalgety	NSW	-36.530	148.780	SAMA	R42617
ABTC 24201	Litoria verreauxii	-	y	Watagan State Forest	NSW	-33.120	151.170	SAMA	R33929
ABTC 24897	Litoria verreauxii	MT497827	-	Watagan State Forest	NSW	-33.120	151.170
ABTC 25248	Litoria verreauxii	OR546042	y	Barrington Tops	NSW	-32.040	151.500
ABTC 25255	Litoria verreauxii	OR546043	y	Mernot State Forest	NSW	-31.680	151.580
ABTC 25275	Litoria verreauxii	OR546044	y	Dingo Tops	NSW	-31.660	152.130
ABTC 86385	Litoria verreauxii	OR546039	-	Myrtleford	Vic	-36.597	146.767	ANWC	A02604
ABTC 99134	Litoria verreauxii	-	y	Marthavale	Vic	-37.400	147.600
AMSR 186628	Litoria verreauxii	OR546033	-	Orroral River	ACT	-35.628	148.958	AMS	AMSR 186628
AMSR 188141	Litoria verreauxii	OR546045	-	Yetholme	NSW	-33.414	149.806	AMS	AMSR 188141
AMSR 188159	Litoria verreauxii	OR546034	-	Darkes Forest	NSW	-34.241	150.938	AMS	AMSR 188159
AMSR 188369	Litoria verreauxii	-	y	Tubbut	Vic	-37.040	148.558	AMS	AMSR 188369
NMVZ 13753	Litoria verreauxii	-	y	Dixons Creek	Vic	-37.608	145.376
NMVZ 57713	Litoria verreauxii	-	y	Dutson	Vic	-38.220	147.275	NMV	D 77286
NMVZ 60371	Litoria verreauxii		y	Wombat Creek	Vic	-37.771	148.243	NMV	D 77302
NMVZ 60407	Litoria verreauxii	-	y	Holey Plains State Park	Vic	-38.248	147.035	NMV	D 77813
ABTC 139703	Litoria watsoni	MT497850	-	Mount Jersey Road, 14.k SSW Bonang	Vic	-37.292	148.643
ABTC 139706	Litoria watsoni	MT497849	-	Yalmy Road, 5.2k SSW Bonang	Vic	-37.226	148.704
ABTC 140298	Litoria watsoni	MT497846	-	Flat Rock Creek, Parma Creek Nature Reserve	NSW	-35.028	150.499	AMS	R177178
ABTC 149194	Litoria watsoni	MT497845	-	Gerringong Falls	NSW	-34.661	150.654
ABTC 17597	Litoria watsoni	MT497848	-	5 km NE Tianjara Falls	NSW	-35.100	150.370	SAMA	R42607