A new genus of the tribe Blondeliini (Diptera: Tachinidae: Exoristinae) from the Oriental Region with morphological and molecular evidence Author Huang, Yu-Zen Author Tachi, Takuji text Zootaxa 2024 2024-08-19 5496 2 151 171 http://dx.doi.org/10.11646/zootaxa.5496.2.1 journal article 301947 10.11646/zootaxa.5496.2.1 8f106167-dad7-4aae-bfbc-50da9df610c4 1175-5326 13346500 FDF92FEA-A841-4CB0-AE31-FC937007DEC0

TABLE S1. PCR protocols for 16S, 28S and white amplification.

DNA polymerase	PCR protocols for 16S and 28S amplification
ExTaq	95℃ for 60 s; 40 cycles of 95℃ for 45 s, 50℃ for 45 s, 72℃ for 60 s; extension at 72℃ for 300 s
Gflex	95℃ for 180 s; 40 cycles of 98℃ for 10 s, 50℃ for 15 s, 68℃ for 60 s; extension at 68℃ for 60 s
Number of rounds and DNA polymerase	PCR protocols for white amplification
First-round for ExTaq	95℃ for 60 s; 40 cycles of 95℃ for 45 s, 45℃ for 45 s, 72℃ for 60 s; extension at 72℃ for 300 s
First-round for Gflex	95℃ for 180 s; 40 cycles of 98℃ for 10 s, 45℃ for 15 s, 68℃ for 60 s; extension at 68℃ for 60 s
Second-round for ExTaq	95℃ for 60 s; 40 cycles of 95℃ for 45 s, 55℃ for 45 s, 72℃ for 60 s; extension at 72℃ for 300 s
Second-round for Gflex	95℃ for 180 s; 40 cycles of 98℃ for 10 s, 55℃ for 15 s, 68℃ for 60 s; extension at 68℃ for 60 s