Systematics of the Little Red Tree Frog, Litoria rubella (Anura: Pelodryadidae), with the description of two new species from eastern Australia and arid Western Australia Author Purser, William A. School of Biological Sciences, The University of Western Australia, Perth, WA 6009, Australia & Phoenix Environmental Sciences, 2 / 3 King Edward Road, Osborne Park, WA 6017, Australia Author Doughty, Paul Collections & Research, Western Australian Museum, 49 Kew Street, Welshpool, WA 6106, Australia Author Rowley, Jodi L. Australian Museum Research Institute, Australian Museum, 1 William Street, Sydney, NSW 2010, Australia & Centre for Ecosystem Science; School of Biological, Earth and Environmental Sciences, University of New South Wales, Sydney, NSW 2052, Australia Author Böhme, Wolfgang Zoologisches Forschungsmuseum Alexander Koenig, Bonn, Germany Author Donnellan, Stephen C. South Australian Museum, North Terrace, Adelaide, SA 5000, Australia Author Mitchell, Marion Anstis Nicola Author Shea, Glenn M. Australian Museum Research Institute, Australian Museum, 1 William Street, Sydney, NSW 2010, Australia & Sydney School of Veterinary Science B 01, University of Sydney, NSW 2006, Australia Author Amey, Andrew Biodiversity and Geosciences, Queensland Museum, PO Box 3300, South Brisbane BC, Queensland 4101, Australia Author Mitchell, Brittany A. Australian Museum Research Institute, Australian Museum, 1 William Street, Sydney, NSW 2010, Australia & Centre for Ecosystem Science; School of Biological, Earth and Environmental Sciences, University of New South Wales, Sydney, NSW 2052, Australia Author Catullo, Renee A. School of Biological Sciences, The University of Western Australia, Perth, WA 6009, Australia & Collections & Research, Western Australian Museum, 49 Kew Street, Welshpool, WA 6106, Australia & Australian Museum Research Institute, Australian Museum, 1 William Street, Sydney, NSW 2010, Australia text Zootaxa 2025 2025-02-28 5594 2 269 315 https://doi.org/10.11646/zootaxa.5594.2.3 journal article 308469 10.11646/zootaxa.5594.2.3 21ed7d07-59b0-42ef-982b-b9b4a1c8764e 1175-5326 14954346 5D61C55E-D042-4818-9D7B-087DEC250BFB

TABLE 3. Diagnostic nucleotide differences between species within the L. rubella species complex in the mitochondrial ND4 gene. Nucleotide positions are numbered with respect to the Phyllomedusa bahiana mitochondrial genome (GenBank accession: NC_067554).

	1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1
	1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1
	0 1 1 1 1 1 1 1 1 2 2 2 2 3 3 3 3 3 3 5 5 5 5 5 5 6 6 6 6 6 6 6 6 7
	5 0 0 1 3 5 6 6 8 0 4 5 6 0 2 3 5 7 8 0 4 4 5 6 6 3 4 5 6 6 8 8 8 0
	6 1 4 6 8 5 2 5 2 7 8 1 6 8 6 9 3 4 0 3 2 6 1 7 9 2 7 9 5 8 0 3 9 1
L. rubella	C G A C A T T T C C C A A A/C C/T C T A T A T T G/A A C A/G T T G/T C T T C A
L. pyrina sp. nov.	C G A C A T T T T C T A A G A C C A C A C C T A T A/G C T C T T A T T
L. larisonans sp. nov.	C A G T A C T T C C C A G G A T T G T C C T T G T A T C C T C C T A
L. capitula	T A A C G T C C C T T C A G A C T A T A C C T A T T T T T T T T T -