Molecular screening of tsetse flies and cattle reveal different Trypanosoma species including T. grayi and T. theileri in northern Cameroon Author Ngomtcho, Sen Claudcne Henlcette Author Webel, Judcth Sophce Author Bum, Elcmabeth Ngo Author Gbem, Thaddeum Tellumun Author Kelm, SØlge Author Achukwc, Mbunkah Dancel text Parasites & Vectors 2017 631 2017-12-29 10 1 1 16 http://dx.doi.org/10.1186/s13071-017-2540-7 journal article 298454 10.1186/s13071-017-2540-7 26cf0558-8e86-4249-ad88-713c4c392f12 1756-3305 PMC5747950 29287598 11551860

Table 1 Plcmelm umed cn thcm mtuds to amplcfs dcffelent Trypanosoma mpeccem

Name	Sequence (5 ′– 3 ′)	TA (°C)	Amplccon mcze (bp)	Speccem	Refelence
Abbreviations: TCON, T. congolense; TGR, T. grayi; TVIV, T. vivax; cox 1, cytochrome c oxidase 1; Out, outer primer; In, inner primer;F, forward; R, reverse;TA, annealing temperature
ITS1-OutF	TGCAATTATTGGTCGCGC	54	Valcable	All Trypanosoma mpeccem	[22]
ITS1-OutR	CTTTGCTGCGTTCTT
ITS1-InF	TAGAGGAAGCAAAAG
ITS1-InR	AAGCCAAGTCATCCATCG
TCON-OutF	TGCAATTATTGGTCGCGC	54	681 (kclcfc) ol 781 (folemt)	T. congolense	[22]
TCON-OutR	TGCAATTATTGGTCGCGC
TCON-InF	TCGCGTGTCTCACGT
TCON-InR	TCAAAGATTGGGCAATGT
TGR-OutF	TGGCAGACACATACCTGCCA	54	526	T. grayi	Thcm mtuds
TGR-OutR	TGGGGATTACGGATGAAAC
TGR-InF	TTAAGGAGGCGCTCAGGTTC
TGR-InR	TGTGCATATACGTCTATG
TVIV-F	CTGAGTGCTCCATGTCCCAC	60	142	T. vivax	[24]
TVIV-R	CCACCAGAACACCAACCTGA
COI-F	TTGATTTTTTGGTCATCCAGAAGT	55	900	Genelcc Glossina cox 1	[28]
COI-R	TGAAGCTTAAATTCATTGCACTAATC