Biodiversity and Biogeography of Chthamalid Barnacles from the North-Eastern Pacific (Crustacea Cirripedia) Author Benny K. K. Chan Author H. - N. Chen Author P. R. Dando Author A. J. Southward Author E. C. Southward text PLoS ONE 2016 2016-03-09 11 3 1 51 journal article 30901 10.1371/journal.pone.0149556 f02345d5-4425-434a-8574-883833ae0b6f PMC4784953 26958842 1161582

Table 2. Enymes compared in the present study and electrophoresis conditions.

Enzyme	Abbreviation	Enzyme Catalogue no.	Buffer	Additive
Buffers used: 1. 40 mM citric acid adjusted to pH 7.7 with N-(3-aminopropyl) diethanolamine for electrode compartments, 1 in 20 dilution for the gel. 2. 900 mM 2-amino-2-hydroxymethyl-propane-1, 3-diol, 500 mM boric acid, 20 mM EDTA pH 8.7. Additives added to the gel: 0.1 mM NAD, 1 mM ATP or 0.1 mM FDP doi:10.1371/journal.pone.0149556.t002
Malate dehydrogenase	Mdh1, Mdh2	1.1.1.37	1	NAD
Malic enzyme	Me	1.1.1.39	2	none
Phosphogluconatedehydrogenase (decarboxylating)	Pgdh	1.1.1.41	2	ATP
Glyceraldehydephosphate dehydrogenase	Gpdh	1.2.1.12	1	NAD
Superoxide dismutase	Sod	1.15.1.1	2	ATP
Hexokinase	Hk	2.7.1.1	2	ATP
Pyruvate kinase	Pk	2.7.1.40	2	FDP
Phosphoglycerate kinase	Pgk	2.7.2.3	2	ATP
Arginine kinase	Ark1, Ark2	2.7.3.3	2	ATP
Phosphoglucomutase	Pgm-1	2.7.5.1	2	FDP
Fructose-bisphosphate aldolase	Ald	4.1.2.13	1	NAD
Enolase	En	4.2.1.11	2	none
Glucosephosphate isomerase	Gpi	5.3.1.9	2	FDP
Mannosephosphate isomerase	Mpi	5.3.1.18	2	FDP