Agaricus radiatosquamulosus sp. nov. of A. subg. Spissicaules from Pakistan Author Bashir, Hira 0000-0002-0917-5224 hira. b 21 @ hotmail. com; https: // orcid. org / 0000 - 0002 - 0917 - 5224 hira.b21@hotmail.com Author Fatima, Nadia 0000-0001-9794-7222 nadiafatima 904 @ gmail. com; https: // orcid. org / 0000 - 0001 - 9794 - 7222 nadiafatima904@gmail.com Author Izhar, Aiman 0000-0002-8739-2881 aimanizhar 25 @ gmail. com; https: // orcid. org / 0000 - 0002 - 8739 - 2881 aimanizhar25@gmail.com Author Niazi, Abdul Rehman 0000-0002-1118-1148 drarniazi. botany @ pu. edu. pk; https: // orcid. org / 0000 - 0002 - 1118 - 1148 drarniazi.botany@pu.edu.pk Author Khalid, Abdul Nasir 0000-0002-5635-8031 drankhalid @ gmail. com; https: // orcid. org / 0000 - 0002 - 5635 - 8031 drankhalid@gmail.com Author Callac, Philippe 0000-0001-6860-0983 phcallac @ laposte. net; https: // orcid. org / 0000 - 0001 - 6860 - 0983 phcallac@laposte.net text Phytotaxa 2023 2023-01-24 579 4 231 242 http://dx.doi.org/10.11646/phytotaxa.579.4.1 journal article 20941 10.11646/phytotaxa.579.4.1 c4b3c78b-2406-497e-9515-b8ce2e27078f 1179-3163 7563931

TABLE 1. List of Agaricus samples with GenBank accession numbers used in the phylogenetic analyses (new species are in bold).

Taxon	Sample number	ITS GenBank	Country: state, province,	Reference
accession number or region
A. subg. Spissicaules
A. sect. Subrutilescentes
a In absence of any taxonomic study, this name remains provisional as it is only based on the identification given in GenBank (MF461606) for strain FUNN2017/1916 (PDD) as A. lanatoniger by P. B. Matheny. b In multigene analyses of Ortiz-Santana et al. (2021) this sample formed a monotypic section. c Only the two samples from Pakistan were morphologically examined.
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a Ten non-referenced sequences are listed in the Table according to their phylogenetic relationships in the ITS tree. Five of those were already included in the analyses of Ortiz-Santana et al. (2021) for diverse reasons. We retrieved five additional nonreferenced sequences in this study: one sequence from New Zealand (FUNNZ2017/1916) matching with another sequence from this country already in our sample (TL2321), and four sequences from India (BAB4729, BAB-4736, BAB-4970, BAB- 4975) matching with our new specimens from Pakistan (BG01 and N-224).
A. sp.	isolate 119	JN182898	China: Sichuan Basin	Non-referenceda
A. linzhiensis	ZRL2012618 T	KT951378	China: Tibet	Zhao et al. 2016
A. ciferrianus	PR6822 T	MT436327	Puerto Rico	Ortiz-Santana et al. 2021
A. ciferrianus	PR6585	MT436328	Puerto Rico	Ortiz-Santana et al. 2021
A. subrutilescens	RWK2122	KJ877775	USA: California	Kerrigan 2016; Ortiz-Santana et
				al. 2021
A. thiersii	UBC F16985	MF954624	Canada: British Columbia	Non-referenceda
A. thiersii	RWK1940	AY943973	USA: California	Kerrigan et al. 2005
A. sp.	ZRL2014289	KT951418	China: Yunnan	Zhao et al. 2016
		accession number	or region
A. parasubrutilescens	ZRL2014076 T	KT951410	China: Yunnan	Zhao et al. 2016
A. parasubrutilescens	ZRL2011027	KT951350	China: Yunnan	Zhao et al. 2016
A. cf. lanatoniger	TL2321	JF495040	New Zealand	Lebel and Syme 2012
A. cf. lanatoniger	FUNNZ2017/1916	MF461606	New Zealand	Non-referenceda
A. inthanonensis	ZRLLD014 T	KT951422	Thailand	Zhao et al. 2016
A. inthanonensis	LDMS22	KT951423	China: Yunnan	Zhao et al. 2016
A. brunneopileatus	ZRL2012115 T	KT951404	China: Yunnan	Zhao et al. 2016
A. brunneopileatus	ZRL2014144	KT951414	China: Yunnan	Zhao et al. 2016
A. planiceps	ZRL20151566 T	KX186720	China: Zhejiang	Dai et al. 2016
A. planiceps	ZRL2015166	KX186722	China: Fujian	Dai et al. 2016
A. vinosobrunneofumidus	RWK2012	KJ877781	USA: Tennessee	Kerrigan 2016; Ortiz-Santana et
				al. 2021
A. vinosobrunneofumidus	NC-1-8341	AY456333	USA: North Carolina	Edwards et al. 2004
A. vinosobrunneofumidus	MGW1363	KY777365	USA: Tennessee	Ortiz-Santana et al. 2021
A. aff. subrutilescens	CB08378	KT874933	Mexico: Mexico State	Non-referenceda
A. aff. subrutilescens	HC-PNNT-049	KT874934	Mexico: Mexico State	Non-referenceda
A. sp.	CA583	JF797192	France	Zhao et al. 2011
A. catenariocystidiosus	ZRL2015038 T	KX186706	China: Zhejiang	Dai et al. 2016
A. catenariocystidiosus	ZRL20151210	KX186713	China: Zhejiang	Dai et al. 2016
A. sp. 1	PR6226	MT436329	Puerto Rico	Ortiz-Santana et al. 2021
A. sp. 1	PR6202	MT436330	Puerto Rico	Ortiz-Santana et al. 2021
A. sp. 1	PR6214	MT436331	Puerto Rico	Ortiz-Santana et al. 2021
A. omphalodiscus	F2255 T	JF797198	France: Martinique	Zhao et al. 2016, Ortiz-Santana
				et al. 2021
A. sect. A
A. sp. 2	JBSD130759	MT436332	Dominican Republic	Ortiz-Santana et al. 2021
A. sect. Spissicaules
A. lanipedisimilis	ZRL2012193 T	KT951399	China: Yunnan	Zhao et al. 2016
A. lanipedisimilis	ZRL2012151	KT951366	China: Yunnan	Zhao et al. 2016
A. planipileus	ZRL20151241	KX186717	China: Zhejiang	Dai et al. 2016
A. planipileus	ZRL2011250 T	KT951398	China: Yunnan	Zhao et al. 2016
A. litoraloides	SHY2011073026 T	KT951408	China: Yunnan	Zhao et al. 2016
A. litoraloides	ZRL2011249	KT951353	China: Yunnan	Zhao et al. 2016
A. sp.	RCGUY07019	JF797199	French Guiana	Zhao et al. 2011
A. bresadolanus	CA177	DQ185570	France	Kerrigan et al. 2005
A. bresadolanus	CA10	DQ185569	France	Kerrigan et al. 2005
A. bresadolanus	CA575	JQ824134	France	Foulongne-Oriol et al. 2012
A. beijingensis	ZRLQHY43	KT951386	China: Shandong	Ling et al. 2021
A. beijingensis	ZRLZLJ384255 T	MN873001	China: Beijing	Ling et al. 2021
A. litoralis	LAPAG420	KT951327	Spain	Zhao et al. 2016
A. litoralis	CA120	JN204436	France	Zhao et al. 2012
A. litoralis	strain 816	AJ884642	Israel	Didukh et al. 2005
A. lanipes	LAPAG585	MT436333	Spain	Ortiz-Santana et al. 2021
A. lanipes	CA406	JF797190	France	Zhao et al. 2011
A. sp.	ZRL2012657	KT951406	China: Tibet	Zhao et al. 2016
A. sect. Fulventes
A. bellanniae	CA486	JF797189	France	Zhao et al. 2011, Ortiz-Santana
				et al. 2021
A. bellanniae	RWK2008 T	KJ877782	USA: Pennsylvania	Kerrigan 2016
		accession number or region
A. bellanniae	MKuoCon5	KJ877783	USA: Illinois	Kerrigan 2016
A. nanofulvens	AH47621 T	MT436334	Argentina	Ortiz-Santana et al. 2021
A. sp.	ADK2171	JF514517	Benin	Zhao et al. 2011
A. radiatosquamulosus	CBS 623.89	MH862192	India	Vu et al. 2019
A. radiatosquamulosus	BAB-4729	KR154958	India	Non-referenceda
A. radiatosquamulosus	BAB-4736	KR154965	India	Non-referenceda
A. radiatosquamulosus	BAB-4970	KR155060	India	Non-referenceda
A. radiatosquamulosus	BAB-4975	KR155065	India	Non-referenceda
A. radiatosquamulosus	BG01 T		Pakistan	This study
A. radiatosquamulosus	N-224		Pakistan	This study
A. sect. Globoterminales
A. basicingulatus	JBSD130754 T	MT436338	Dominican Republic	Ortiz-Santana et al. 2021
A. basicingulatus	JBSD130766	MT436341	Dominican Republic	Ortiz-Santana et al. 2021
A. sect. Amoeni
A. kivuensis	Rammeloo5756	JF514541	Burundi	Zhao et al. 2011
A. sp.	F2467	JF797200	France: Martinique	Zhao et al. 2011
A. gratolens	ZRL3093 T	JF691548	Thailand	Zhao et al. 2011; 2016
A. gratolens	NB72	EU118124	India: Nilgiri Biosphere	Non-referenceda
A. brunneovariabilis	LAPAF3	JF797184	Reserve Togo	Zhao et al. 2011, Ling et al.
				2021
A. brunneovariabilis	ZRL2014277 T	MN872985	Yunnan, China	Ling et al. 2021
A. suthepensis	NTT42	JF514538	Thailand	Zhao et al. 2011
A. suthepensis	LD2012100 T	KT951409	Thailand	Zhao et al. 2016
A. amoenomyces	ZRL2010072 T	KT951348	China: Yunnan	Zhao et al. 2016
A. sp.	NYS122	JF514540	Benin	Zhao et al. 2011
A. hanthanaensis	SK2012 T	JQ246405	Sri Lanka	Liu et al. 2015
A. hanthanaensis	GKVK-10	MF403064	India: Karnataka	Mariselvi and Earanna 2018
A. sect. Rarolentes
A. furfuripes	JBSD127435 T	MT436343	Dominican Republic	Ortiz-Santana et al. 2021
A. furfuripes	DR2674	MT436344	Dominican Republic	Ortiz-Santana et al. 2021
A. microincrustatus	PR6809	MT436346	Puerto Rico	Ortiz-Santana et al. 2021
A. microincrustatus	OKM27279	MT436348	Puerto Rico	Ortiz-Santana et al. 2021
A. microincrustatus	PR6877 T	MT436349	Puerto Rico	Ortiz-Santana et al. 2021
A. butyreburneus	RWK2015	KC135887	USA: Tennessee	Kerrigan 2016
A. butyreburneus	BOS801	MT436350	USA: New York	Ortiz-Santana et al. 2021
A. butyreburneus	CA279	JF797191	France	Zhao et al. 2011
A. parvisporus	PR6173 T	MT436351	Puerto Rico	Ortiz-Santana et al. 2021
A. baronii	DR2523 T	MT436352	Dominican Republic	Ortiz-Santana et al. 2021
A. albosquamosus	LD2012192 T	KT951394	Thailand	Zhao et al. 2016
A. albosquamosus	LD201235	KT951395	Thailand	Zhao et al. 2016
A. leucolepidotus	LD201214 T	KT951336	Thailand	Zhao et al. 2016
A. brunneodiscus	TBGT16727	MZ437441	India	Arya et al. 2022
A. aureoelephanti	MEL2382860 T	KP012734	Australia	Crous et al. 2022
Outgroup
A. campestris	LAPAG370 T	JQ903618	Spain	Kerrigan 2016; Parra et al.
				2016; Zhao et al. 2016