Carotenoid profiles of red- and yellow-colored arils of cultivars of Taxus baccata L. and Taxus × media Rehder Author Schex, Roland DSM Nutritional Products, Research and Development Center Forms, P. O. Box 2676, 4002, Basel, Switzerland & * & Geisenheim University, Institute of Beverage Research, Analysis and Technology of Plant-based Foods, Von-Lade-Strasse 1, 65366, Germany Author Lieb, Veronika M. University of Hohenheim, Institute of Food Science and Biotechnology, Plant Foodstuff Technology and Analysis, Garbenstrasse 25, 70599, Stuttgart, Germany & DSM Nutritional Products, Research and Development Center Forms, P. O. Box 2676, 4002, Basel, Switzerland Author Schafer, Christian Author Schweiggert, Ralf * & Geisenheim University, Institute of Beverage Research, Analysis and Technology of Plant-based Foods, Von-Lade-Strasse 1, 65366, Germany Author Steingass, Christof B. * & Geisenheim University, Institute of Beverage Research, Analysis and Technology of Plant-based Foods, Von-Lade-Strasse 1, 65366, Germany text Phytochemistry 2021 112741 2021-06-30 186 1 11 http://dx.doi.org/10.1016/j.phytochem.2021.112741 journal article 10.1016/j.phytochem.2021.112741 1873-3700 8273180
Table 2 HPLC-DAD-ESI-MS n data of free carotenoids extracted from red- and yellow-colored arils of cultivars of Taxus baccata L. and Taxus × media Rehder.
No.t R (min)Proposed structureλ maxD/Da (%) B IID/Db (%) III II[M] +• (m/z)[M+H] + (m/z)ESI(+)-MS n experiment
t, retention time; λ, UV/vis absorption maxima; sh, shoulder; n.d., not detected; * base peak in the ESI(+)-MS n experiment. R max Given t R and UV/vis data were acquired using the settings and instrumentation detailed in section 4.6, the MS data as specified in section 4.5. a D/Dratio of absorbance at the ‘(Z)-peak’ (D) and the main absorption maximum (D). B II, B II b D/Dratio of absorbance at maximum at longest wavelength (D) to D. III II, III II c In-source elimination of water ([M + H – H O] +). 2 d Verified by an authentic reference standard. e Verified by a thermally isomerized reference standard of rhodoxanthin. f Verified by a reference compound extracted from the petals of Eschscholzia californica Cham.
(nm)(m/z)
14.9(all- E)-Violaxanthin d266, 418, 440, 470–87600601[601]: 583*, 565, 509, 491, 221
27.1(all- E)-Lutein dsh420, 446, 474–65568569 551c[551]: 533*, 495, 429, 411
37.7(all- E)-Zeaxanthin dsh424, 452, 478–30568569[569]: 551, 477, 476*
48.2Unknownsh424, 446, 474–57n.d.n.d.n.d.
58.8Rhodoxanthin isomer e316, 47819–n.d.563[563]: 545, 507, 479, 425, 389, 359*, 281, 209
69.2Rhodoxanthin isomer e336, 48622–n.d.563[563]: 545, 507, 479, 425, 389, 359*, 281, 209
79.4Unknown266, 418, 440, 470–99n.d.n.d.n.d.
89.4Rhodoxanthin isomer e322, 48216–n.d.563[563]: 545, 507, 479, 425, 389, 359*, 281, 209
99.6Rhodoxanthin isomer e332, 48417–n.d.563[563]: 545, 507, 479, 425, 389, 359*, 281, 209
109.7Eschscholtzxanthin isomer308, 442, 466, 494–39n.d.567 549c[549]: 531*, 475, 457, 439, 409, 223, 209, 187
119.8Rhodoxanthin isomer e334, 49423–n.d.563[563]: 545, 507, 479, 425, 389, 359*, 281, 209
1210.1(6 Z,6′ Z)-Rhodoxanthin e326, 48616–n.d.563[563]: 545, 507, 479, 425, 389, 359*, 281, 209
1310.2Eschscholtzxanthin isomer308, 446, 470, 498–24n.d.549 c[549]: 531*, 475, 457, 439, 409, 223, 209, 187
1410.3Rhodoxanthin isomer e336, 49418–n.d.563[563]: 545, 507, 479, 425, 389, 359*, 281, 209
1510.6Eschscholtzxanthin isomer308, 446, 470, 498–36n.d.567 549c[549]: 531*, 475, 457, 439, 409, 223, 209, 187
1610.7(6 Z)-Rhodoxanthin e330, 49415–n.d.563[563]: 545, 507, 479, 425, 389, 359*, 281, 209
1711.0Eschscholtzxanthin isomer448, 472, sh498–n.d.n.d.567 549 c[549]: 531*, 475, 457, 439, 409, 223, 209, 187
1811.4(all- E)-β- Carotene dsh428, 452, 478–41536537[536]: 444*, 399
1911.5(all- E)-Rhodoxanthin d336, 50215–n.d.563[563]: 545, 507, 479, 425, 389, 359*, 281, 209
2011.6Eschscholtzxanthin isomer308, 448, 472, 502–37n.d.567 549 c[549]: 531*, 475, 457, 439, 409, 223, 209, 187
2112.1(all- E)-Eschscholtzxanthin f308, 448, 472, 502–44n.d.567 549c[549]: 531*, 475, 457, 439, 409, 223, 209, 187