Towards Sustainable Pest Management: Toxicity, Biochemical Effects, and Molecular Docking Analysis of Ocimum basilicum (Lamiaceae) Essential Oil on Agrotis ipsilon and Spodoptera littoralis (Lepidoptera: Noctuidae) Author AWad, Mona Dept of Biology, College of Science and Humanities, Prince Author Nancy, Amer · Author Hassan, N. Author M., Moataz A. text Neotropical Entomology 2024 2024-03-13 53 3 669 681 https://doi.org/10.1007/s13744-024-01137-6 journal article 310153 10.1007/s13744-024-01137-6 745b5858-5367-40e6-87ed-1289433aa2b0 1678-8052 PMC11074029 38478300 15557600

Table 5 The activity of detoxification enzymes (carboxylesterase (α- and β-esterase), cytochrome P-450, and GST) after 24, 48, 72, and 96 h of treating the 2nd instar larvae of Spodoptera littoralis with LC 15 and LC 50 of Ocimum basilicum essential oil

Enzymes	Treatments	Mean± SD
		Hours after treatments
		24 h	48 h	72 h	96 h
α-esterase (µmole/mg of protein)	Control	0.30a ±0.032	0.13a ± 0.001	0.17a± 0.010	0.22a ±0.057
	LC15	0.07b ±0.005	0.08b ± 0.003	0.11b± 0.014	0.23a ±0.037
	LC50	0.06b ±0.013	0.09b ± 0.007	0.09b± 0.014	0.23a ±0.017
β-esterase (µmole/mg of protein)	Control	0.37a ±0.373	0.35a ± 0.035	0.33a± 0.023	0.49a ±0.083
	LC15	0.14a ±0.034	0.21b ± 0.045	0.25b± 0.011	0.29b± 0.023
	LC50	0.18a ±0.007	0.18b ± 0.017	0.23b± 0.027	0.26b± 0.01
Cytochrome P-450 (µmole/min /mg	Control	0.010b± 0.0007	0.006b± 0.0002	0.003b ±0.0001	0.007a ±0.0002
of protein)	LC15	0.010b± 0.0002	0.008ab ±0.0002	0.006a ±0.001	0.014a ±0.0027
	LC50	0.015a± 0.0017	0.010a ±0.002	0.006a ±0.0009	0.008a ±0.0031
GST (µmol/ml/mg of protein)	Control	19.48a ±1.23	11.75a ± 1.575	17.41a± 1.834	19.57a ±2.951
	LC15	4.28b ±0.579	2.95b ± 0.449	3.30b± 0.774	3.71b± 0.705
	LC50	5.17b ±1.128	4.76b ± 1.084	4.84b± 0.577	3.22b± 0.722