Towards Sustainable Pest Management: Toxicity, Biochemical Effects, and Molecular Docking Analysis of Ocimum basilicum (Lamiaceae) Essential Oil on Agrotis ipsilon and Spodoptera littoralis (Lepidoptera: Noctuidae) Author AWad, Mona Dept of Biology, College of Science and Humanities, Prince Author Nancy, Amer · Author Hassan, N. Author M., Moataz A. text Neotropical Entomology 2024 2024-03-13 53 3 669 681 https://doi.org/10.1007/s13744-024-01137-6 journal article 310153 10.1007/s13744-024-01137-6 745b5858-5367-40e6-87ed-1289433aa2b0 1678-8052 PMC11074029 38478300 15557600

Table 4 The activity of detoxification enzymes (carboxylesterase (α- and β-esterase), cytochrome P-450, and GST) after 24, 48, 72, and 96 h of treating the 2nd instar larvae of Agrotis ipsilon with LC 15 and LC 50 of Ocimum basilicum essential oil

Enzymes	Treatments	Mean±SD
		Hours after treatments
		24 h	48 h	72 h	96 h
α-esterase (µmole/mg of protein)	Control	0.08b ±0.010	0.12a ±0.020	0.16a ±0.03	0.19ab± 0.01
	LC15	0.12ab ±0.010	0.23a ±0.029	0.23a ±0.02	0.25a± 0.04
	LC50	0.15a ±0.034	0.22a ±0.056	0.19a ±0.03	0.14b± 0.03
β-esterase (µmole/mg of protein)	Control	0.14a ±0.033	0.12b ±0.022	0.18b± 0.002	0.25ab ±0.009
	LC15	0.24a ±0.063	0.34a ±0.035	0.33a ±0.033	0.29a± 0.028
	LC50	0.26a ±0.013	0.29a ±0.037	0.27a ±0.024	0.19b ±0.028
Cytochrome P-450 (µmole/min /mg	Control	0.007c ±0.0004	0.008a± 0.0003	0.005b ±0.0005	0.011a ±0.001
of protein)	LC15	0.011b ±0.0003	0.012a± 0.0021	0.008a ±0.001	0.017a ±0.001
	LC50	0.016a ±0.0017	0.011a± 0.0019	0.007ab ±0.0002	0.012a ±0.004
GST (µmol/ml/mg of protein)	Control	20.55b ±5.046	21.23c ±6.153	35.78b± 1.80	47.32b ±5.391
	LC15	63.03a ±17.725	51.44b ±8.047	93.08a ±8.10	96.42a± 11.88
	LC50	90.45a ±13.787	90.0a ±6.169	86.74a ±12.17	100.8a ± 7.172