A new leafhopper genus and species on bamboo, described from Europe (Hemiptera, Cicadellidae, Deltocephalinae, Mukariini), with remarkable seasonal dimorphism Author Sanna, Francesco University of Padua, Department of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE), Viale dell’Università 16, 35020, Legnaro, Padova, Italy. Author Holzinger, Werner E. Oekoteam - Institute for Animal Ecology and Landscape Planning, Bergmanngasse 22, 8010 Graz, Austria. Author Cavagna, Francesca University of Verona, Department of Biotechnology, Strada Le Grazie 15, 37134, Verona, Italy. Author Callot, Henry Musée Zoologique de l’Université et de la Ville de Strasbourg, 29 Boulevard de la Victoire, 67000 Strasbourg, France. Author Webb, Michael D. Department of Science (Insects), The Natural History Museum, London, SW 7 5 BD, UK. & Corresponding author, retired text Zootaxa 2025 2025-02-14 5588 2 323 338 https://doi.org/10.11646/zootaxa.5588.2.7 journal article 308341 10.11646/zootaxa.5588.2.7 f9d4791e-45a7-43a5-904d-4663a4a99af8 1175-5326 14896534 59550872-DAA1-4A14-8825-45A8B8624F55

TABLE 2. PCR conditions for each of the investigated regions.

Region	PCR conditions
COI	30 s at 94 °C, 5x (10 s at 94 °C, 20 s at 45 °C, 25 s at 72 °C), 35x (10 s at 94 °C, 20 s at 52 °C, 25 s at 72 °C), 2 min at 72 °C
Cytb	30s at 94°C, 35x (10 s at 94 °C, 20 s at 58 °C, 14 s at 72 °C), 2 min at 72 °C
16s	30s at 94°C, 35x (10 sat 94 °C, 20 sat 59 °C, 16 sat 72 °C), 2 min at 72 °C
ITS2	5 min at 96 °C, 35x (1 min at 96 °C, 1 min at 50 °C, 1.5 min at 72 °C), 5 min at 72 °C