Morphological and molecular analysis of Willowsia nigromaculata (Collembola, Entomobryidae, Entomobryinae) reveals a new cryptic species from the United States Author Cipola, Nikolas G. 70DD3D02-82A6-466D-B222-8A30DF9D933F Laboratório de Sistemática e Ecologia de Invertebrados do Solo, Instituto Nacional de Pesquisas da Amazônia – INPA, CPEN. Av. André Araújo, 2936, Aleixo, CEP 69067 - 375, Manaus, AM, Brazil nikolasgc@gmail.com Author Katz, Aron D. 72328BBA-0AF8-41D9-813C-A21EEC7C90C5 Engineer Research and Development Center, 2902 Newmark Drive, Champaign, IL 61826, USA. & Illinois Natural History Survey, Prairie Research Institute, University of Illinois at Urbana- Champaign, 1816 South Oak Street, Champaign, IL 61820, USA. aronkatz@illinois.edu text European Journal of Taxonomy 2021 2021-03-16 739 1 92 116 http://dx.doi.org/10.5852/ejt.2021.739.1269 journal article 7698 10.5852/ejt.2021.739.1269 c8622ca7-cde1-46e9-8c54-da13e2637cc3 2118-9773 4611539 4C7F00E0-9567-4E1D-8CAB-AB3724537DC9

Table 1 (continued on next page). List of Entomobryinae species used for phylogenetic analysis, including locality and COI sequence information, i.e., GenBank accession number and sequence length (bp = base pairs). Abbreviations: AH = Anhui;, AL = Alabama; AM = Amazonas; AZ = Azores; BA = Bahia; BR = Brazil; CA = Canada; CH = China; FL = Florida; FR = France; GD = Guangdong; HB = Hubei; HN = Hunan; IDF = Île-de-France; IL = Illinois; JL = Jilin; NC = North Carolina; OOC = Occitanie; ON = Ontario; PA = Pennsylvania; PI = Piauí; PT = Portugal; TB = Tibet; TN = Tennessee; USA = United States of America, VT = Vermont; WI = Wisconsin; ZJ = Zhejiang.

	Species name	Locality	GenBank #	bp	Reference
1	Coecobrya tenebricosa	Antony, IDF, FR	KM978347	658	Ding et al. 2018
2	Drepanura mogolica	Changling, JL, CH	KY468329	658	Ding et al. 2018
3	Entomobrya assuta	Mason Co., IL, USA	KM610057	1534	Katz et al. 2015a
4	Entomobrya atrocincta	Champaign Co., IL, USA	KM610115	1534	Katz et al. 2015a
5	Entomobrya bicolor	Henderson Co., IL, USA	KM610117	1534	Katz et al. 2015a
6	Entomobrya citrensis	Citrus Co., FL, USA	KM610064	1534	Katz et al. 2015a
7	Entomobrya clitellaria	Coles Co., IL, USA	KM610076	1534	Katz et al. 2015a
8	Entomobrya decemfasciata	Stewart Co., TN, USA	KM610101	1534	Katz et al. 2015a
9	Entomobrya huangi	Nyingchi, TB, CH	KY468314	658	Ding et al. 2018
10	Entomobrya intermedia	Chester Co., PA, USA	KM610121	1534	Katz et al. 2015a
11	Entomobrya jubata	Covington Co., AL, USA	KM610110	1534	Katz et al. 2015a
12	Entomobrya ligata	Swain Co., NC, USA	KM610079	1534	Katz et al. 2015a
13	Entomobrya multifasciata	São Miguel Island, AZ, PT	KM610123	1534	Katz et al. 2015a
14	Entomobrya neotenica	Clay Co., AL, USA	KM610104	1534	Katz et al. 2015a
15	Entomobrya nivalis	Lamoille Co., VT, USA	KM610125	1534	Katz et al. 2015a
16	Entomobrya quadrilineata	Vermilion Co., IL, USA	KM610092	1534	Katz et al. 2015a
17	Entomobrya unifasciata	Chester Co., PA, USA	KM610087	1534	Katz et al. 2015a
18	Entomobrya unostrigata	Champaign Co., IL, USA	MT454111	1530	Katz et al. 2015b
19	Entomobryoides dissimilis	Champaign Co., IL, USA	KM610126	1534	Katz et al. 2015a
20	Homidia sauteri	Butler Co., AL, USA	KM610127	1501	Katz et al. 2015a
21	Homidia socia	Jo Davies Co., IL, USA	KM610128	1534	Katz et al. 2015a
22	Lepidocyrtoides caeruleomaculatus	Rio Preto da Eva, AM, BR	MF716618	1534	Godeiro et al. 2020
23	Lepidocyrtoides sp.	Abaíra, BA, BR	MF716598	1534	Godeiro et al. 2020
24	Lepidodens similis	Shaoguan, GD, CH	KM978396	658	Ding et al. 2018
25	Lepidosira neotropicalis	Piracuruca, PI, BR	MF716603	1534	Nunes et al. 2019
26	Sinhomidia bicolor	Huangshan, AH, CH	KM978375	658	Ding et al. 2018
27	Willowsia buski	Sauk Co., WI, USA	KM610129	1534	Katz et al. 2015a
28	Willowsia fascia	Huanggang, HB, CH	KU833222	658	Ding et al. 2018
29	Willowsia guangdongensis	Guangzhou, GD, CH	KM978377	658	Ding et al. 2018
30	Willowsia japonica	Linhai, ZJ, CH	KM978378	658	Ding et al. 2018
35	Willowsia neonigromaculata sp. nov.	Pope Co., IL, USA	KM610130	1534	Katz et al. 2015a
36	Willowsia neonigromaculata sp. nov.	Brockville, ON, CA	MG034057	542	deWaard et al. 2019
31	Willowsia nigromaculata	Teillet, OCC, FR	KY468311	658	Ding et al. 2018
32	Willowsia nigromaculata	Toronto, ON, CA	MG032239	474	deWaard et al. 2019
33	Willowsia nigromaculata	Toronto, ON, CA	MG036766	594	deWaard et al. 2019
34	Willowsia nigromaculata	Toronto, ON, CA	MG039377	543	deWaard et al. 2019
37	Willowsia pseudobuski	Lu’an, AH, CH	KU833223	658	Ding et al. 2018
38	Willowsia pyrrhopygia	Citrus Co., FL, USA	KM610131	1534	Katz et al. 2015a
39	Willowsia qui	Huangshan, AH, CH	KU833221	658	Ding et al. 2018
40	Willowsia similis	Changsha, HN, CH	KU833225	658	Ding et al. 2018