The transcriptome of sesquiterpenoid biosynthesis in heartwood xylem of Western Australian sandalwood (Santalum spicatum) Author Moniodis, Jessie School of Plant Biology (M 084), University of Western Australia (UWA), 35 Stirling Hwy, Crawley, WA 6009, Australia & School of Chemistry and Biochemistry (M 310), University of Western Australia, 35 Stirling Hwy, Crawley, WA 6009, Australia & Michael Smith Laboratories, University of British Columbia, 2185 East Mall, Vancouver, British Columbia V 6 T 1 Z 4, Canada Author Jones, Christopher G. Author Barbour, E. Liz Author Plummer, Julie A. Author Ghisalberti, Emilio L. Author Bohlmann, Joerg text Phytochemistry 2015 2015-05-31 113 79 86 http://dx.doi.org/10.1016/j.phytochem.2014.12.009 journal article 10.1016/j.phytochem.2014.12.009 1873-3700 10487461

Table 1 Summary of genes identified in the xylem transcriptome encoding enzymes of the MEV and MEP pathway, TPS, cytochrome P450, and allylic phosphatase.

Gene	Function	Contig length (bp)	Number of reads	Best match
				Species	BLAST-X e value
Mevalonic acid pathway
AACT1	Acetyl-CoA thiolase	1227	67	Bacopa monnieri	0.00E+00
AACT2	Acetyl-CoA thiolase	1805	1	Bacopa monnieri	0.00E+00
HMGS	3-Hydroxy-3-methylglutaryl-CoA synthase	1413	34	Camellia sinensis	0.00E+00
HMGR1	3-Hydroxy-3-methylglutaryl-CoA reductase	1909	339	Camptotheca acuminata	0.00E+00
HMGR2	3-Hydroxy-3-methylglutaryl-CoA reductase	2030	1	Camptotheca acuminata	0.00E+00
MK	Mevalonate kinase	1100	18	Hevea brasiliensis	7.00E+159
MVD	Mevalonate 5-diphosphate decarboxylase	843	27	Catharanthus roseus	2.00E-172
MEP pathway
DXS	1-Deoxyxylulose-5-phosphate synthase	777	6	Arabidopsis thaliana	9.00E-159
DXR	1-Deoxyxylulose-5-phosphate reductoisomerase	1303	1	Hevea brasiliensis	1.00E-114
IPP isomerases
IDI	Isopentyl diphosphate isomerase	789	5	Santalum album	5.00E-161
Prenyltransferases
GPPS	Geranyl diphosphate synthase	536	2	Solanum lycopersicum	6.00E-52
FPPS	Farnesyl diphosphate synthase	1154	25	S. album	0.00E+00
GGPPS	Geranylgeranyl diphosphate synthase	798	1	Corylus avellana	1.00E-73
Terpene synthase
Sspi SSy	Santalene synthase	1713	96	Santalum spicatum	0.00E+00
Sspi BS	OE- Bisabolol synthase	1731	57	S. spicatum	0.00E+00
Sspi TPS4	Sesquisabinene B synthase	1701	5	S. album	1.00E-59
Sspi TPS5	Unknown function	765	4	Vitis vinifera	1.00E-48
Sspi TPS6	Unknown function	867	4	S. murrayanum	2.00E-166
Sspi TPS7	Unknown function	746	1	Quercus ilex	2.00E-93
Sspi TPS8	Unknown function	667	1	S. austrocaledonicum	3.00E-84
CYP76 P450s
Sspi CYP76 F39	Santalene hydroxylase	1530	5	S. album	0.00E+00
Phosphatase
	Phosphatase PAP2-like	1181	12	Croton stellatopilosus	1.00E-124