Cryptic diversity in toads of the Rhinella marina species group (Anura, Bufonidae) with a subjectively beautiful new species from Western Ecuador Author Menéndez-Guerrero, Pablo A. Laboratorio de Macroecología y Cambio Global, Facultad de Ciencias Exactas y Naturales, Pontificia Universidad Católica del Ecuador, Avenida 12 de Octubre 1076 y Roca, Apartado Postal 17 - 01 - 2184, Quito, Ecuador & Redpath Museum, McGill University, 859 Sherbrooke Street West, Montreal, QC, H 3 A 2 K 6, Canada Author Lima, Sueny P. Author Santos Museo de Zoología, Pontificia Universidad Católica del Ecuador, Avenida 12 de Octubre 1076 y Roca, Apartado Postal 17 - 01 - 2184, Quito, Ecuador & Translational Biomedical Sciences, Ohio University, 02 Grosvenor Hall, Athens, OH, USA Author Salazar-Nicholls, María-José Museo de Zoología, Pontificia Universidad Católica del Ecuador, Avenida 12 de Octubre 1076 y Roca, Apartado Postal 17 - 01 - 2184, Quito, Ecuador & Department of Biology, Boston University, 5 Cummington Mall, Boston, MA 02215, USA Author Green, David M. Redpath Museum, McGill University, 859 Sherbrooke Street West, Montreal, QC, H 3 A 2 K 6, Canada Author Ron, Santiago R. Museo de Zoología, Pontificia Universidad Católica del Ecuador, Avenida 12 de Octubre 1076 y Roca, Apartado Postal 17 - 01 - 2184, Quito, Ecuador text Zoological Journal of the Linnean Society 2024 2024-02-20 202 3 1 26 https://doi.org/10.1093/zoolinnean/zlad197 journal article 306574 10.1093/zoolinnean/zlad197 046a1646-6c0e-4090-b2fb-1c9a49970aa5 0024-4082 14423165 7-01-2184

Table 6. Loadings, eigenvalues, and percentage of explained variance for four first PCs from a PCA. The analysis is based on 19 climatic variables (BIO1–BIO19) from 98 collection localities of three toad populations’ specimens used for the phylogenetic analysis (see Material and methods section)

Variables	PC I	PC II	PC III	PC IV
BIO1—Annual Mean Temperature	0.989	0.103	0.086	-0.045
BIO2—Mean Diurnal Range [Mean (period max-min)]	-0.056	-0.229	-0.233	0.865
BIO3—Isothermality	0.13	0.306	0.879	-0.077
BIO4—Temperature Seasonality (Coefficient of Variation)	-0.049	-0.228	-0.908	0.182
BIO5—Max Temperature of Warmest Period	0.97	0.004	-0.149	0.181
BIO6—Min Temperature of Coldest Period	0.881	0.196	0.342	-0.259
BIO7—Temperature Annual Range	-0.121	-0.294	-0.709	0.623
BIO8—Mean Temperature of Wettest Quarter	0.988	-0.003	-0.068	-0.071
BIO9—Mean Temperature of Driest Quarter	0.949	0.215	0.192	-0.033
BIO10—Mean Temperature of Warmest Quarter	0.993	0.046	-0.085	-0.019
BIO11—Mean Temperature of Coldest Quarter	0.944	0.13	0.281	-0.092
BIO12—Annual Precipitation	0.129	0.954	0.237	-0.104
BIO13—Precipitation of Wettest Period	0.072	0.866	-0.117	-0.316
BIO14—Precipitation of Driest Period	0.09	0.889	0.347	0.039
BIO15—Precipitation Seasonality (Coefficient of Variation)	0.032	-0.795	-0.471	-0.088
BIO16—Precipitation of Wettest Quarter	0.139	0.892	-0.028	-0.308
BIO17—Precipitation of Driest Quarter	0.076	0.894	0.355	0.026
BIO18—Precipitation of Warmest Quarter	0.077	0.714	0.018	-0.383
BIO19 -Precipitation of Coldest Quarter	0.174	0.832	0.405	0.018
Eigenvalues	9.118	5.394	1.942	1.19
% of variance explained	34.62	33.04	16.49	8.71
Cumulative %	34.62	67.66	84.15	92.86